2024
Neuropeptide and serotonin co-transmission sets the activity pattern in the C. elegans egg-laying circuit
Butt A, Van Damme S, Santiago E, Olson A, Beets I, Koelle M. Neuropeptide and serotonin co-transmission sets the activity pattern in the C. elegans egg-laying circuit. Current Biology 2024, 34: 4704-4714.e5. PMID: 39395419, DOI: 10.1016/j.cub.2024.07.064.Peer-Reviewed Original ResearchHermaphrodite-specific neuronsEgg-laying circuitNlp-3Egg layingTiming of egg layingPromote egg layingCaenorhabditis elegansInactive phasePeptide signalsPersistent internal stateNeural circuit functionMolecular experimentsSerotonin receptorsSerotonin signalingSerotoninMuscle cellsNeurotransmitter serotoninMultiple signalsNeuropeptideNeuropeptide receptorsCo-transmissionEggsNeural circuitsLayingActive phase
2023
Multiple Subthreshold GPCR Signals Combined by the G-Proteins Gαq and Gαs Activate the Caenorhabditis elegans Egg-Laying Muscles
Olson A, Butt A, Christie N, Shelar A, Koelle M. Multiple Subthreshold GPCR Signals Combined by the G-Proteins Gαq and Gαs Activate the Caenorhabditis elegans Egg-Laying Muscles. Journal Of Neuroscience 2023, 43: 3789-3806. PMID: 37055179, PMCID: PMC10219013, DOI: 10.1523/jneurosci.2301-22.2023.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsMultiple G protein-coupled receptorsMuscle cellsMuscle activitySerotonin G protein-coupled receptorsDesigner G protein-coupled receptorsIntact animalsG proteinsEndogenous G protein-coupled receptorsIndividual G protein-coupled receptorsGPCR signalsCalcium activityEgg-laying musclesSerotoninIndividual neuronsDouble knockoutNeuronsBehavioral outcomesMuscleMost cellsG protein GαqCellsSubthreshold signalEgg-laying systemSER-1
2019
Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying
Brewer JC, Olson AC, Collins KM, Koelle MR. Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying. PLOS Genetics 2019, 15: e1007896. PMID: 30677018, PMCID: PMC6363226, DOI: 10.1371/journal.pgen.1007896.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying defectsNLP-3C. elegansEgg-laying musclesEgg-laying circuitDirect postsynaptic targetsEgg-laying behaviorSerotonergic Hermaphrodite Specific NeuronsMuscle cellsSmall molecule neurotransmittersNull mutantsHSN neuronsDouble mutantSingle mutantsMutant animalsSerotonergic neuronsWild-type animalsSevere defectsMutantsElegansNeuropeptide substance PMammalian brainEggsSpecific neurons