2023
An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity
Ader N, Chen L, Surovtsev I, Chadwick W, Rodriguez E, King M, Lusk C. An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity. Nature Cell Biology 2023, 25: 1465-1477. PMID: 37783794, PMCID: PMC11365527, DOI: 10.1038/s41556-023-01235-4.Peer-Reviewed Original ResearchConceptsSpindle pole body proteinNuclear envelope barrierESCRT-III proteinsNuclear pore complexSpindle pole bodyNucleocytoplasmic compartmentalizationESCRT functionPore complexPole bodyDistinct complementNuclear compartmentNuclear integrityTransport proteinsMolecular mechanismsRemodelling mechanismProteinBody protein
2016
Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction
Zhao Y, Schreiner SM, Koo PK, Colombi P, King MC, Mochrie SG. Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction. Biophysical Journal 2016, 111: 19-24. PMID: 27410730, PMCID: PMC4945324, DOI: 10.1016/j.bpj.2016.05.036.Peer-Reviewed Original ResearchConceptsChromatin biologyLac operator arraysMembrane marker proteinsNuclear positionBroad practical utilityNuclear volumeNuclear peripheryOrganelle shapeChromatin markersTranscriptional regulationSubnuclear positionLacI-GFPDNA repairIndividual lociNuclear compartmentPopulation of cellsNuclear envelopeLocus positionParticular locusMarker proteinsImage analysis pipelineLociCell populationsAnalysis pipelineBiological accuracy