2021
Defective Flow-Migration Coupling Causes Arteriovenous Malformations in Hereditary Hemorrhagic Telangiectasia
Park H, Furtado J, Poulet M, Chung M, Yun S, Lee S, Sessa WC, Franco CA, Schwartz MA, Eichmann A. Defective Flow-Migration Coupling Causes Arteriovenous Malformations in Hereditary Hemorrhagic Telangiectasia. Circulation 2021, 144: 805-822. PMID: 34182767, PMCID: PMC8429266, DOI: 10.1161/circulationaha.120.053047.Peer-Reviewed Original ResearchConceptsActivin receptor-like kinase 1Hereditary hemorrhagic telangiectasiaHemorrhagic telangiectasiaVascular malformationsArteriovenous malformationsBlood flowGrowth factor receptor 2Endothelial growth factor receptor 2Vascular endothelial growth factor receptor 2Factor receptor 2Receptor-like kinase 1New potential targetsYAP/TAZ nuclear translocationDeficient miceTransmembrane serine-threonine kinase receptorsDevastating disorderAlk1 deletionReceptor 2Pharmacologic inhibitionCre linesPostnatal retinaMalformationsSerine-threonine kinase receptorsEndothelial cell migrationNuclear translocation
2015
Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex
Coon BG, Baeyens N, Han J, Budatha M, Ross TD, Fang JS, Yun S, Thomas JL, Schwartz MA. Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex. Journal Of Cell Biology 2015, 208: 975-986. PMID: 25800053, PMCID: PMC4384728, DOI: 10.1083/jcb.201408103.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDCadherinsCell MovementCells, CulturedEndothelium, VascularHEK293 CellsHuman Umbilical Vein Endothelial CellsHumansMechanotransduction, CellularMiceMice, Inbred C57BLNeovascularization, PhysiologicPlaque, AtheroscleroticPlatelet Endothelial Cell Adhesion Molecule-1Protein Structure, TertiaryRNA InterferenceRNA, Small InterferingStress, MechanicalStress, PhysiologicalVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth Factor Receptor-3ZO-1 controls endothelial adherens junctions, cell–cell tension, angiogenesis, and barrier formation
Tornavaca O, Chia M, Dufton N, Almagro LO, Conway DE, Randi AM, Schwartz MA, Matter K, Balda MS. ZO-1 controls endothelial adherens junctions, cell–cell tension, angiogenesis, and barrier formation. Journal Of Cell Biology 2015, 208: 821-838. PMID: 25753039, PMCID: PMC4362456, DOI: 10.1083/jcb.201404140.Peer-Reviewed Original ResearchMeSH KeywordsActomyosinAdherens JunctionsAnimalsAntigens, CDCadherinsCapillary PermeabilityCell Adhesion MoleculesCell MovementCells, CulturedClaudin-5Cytoskeletal ProteinsCytoskeletonEndothelial CellsHumansMechanotransduction, CellularMice, Inbred C57BLMyosinsNeovascularization, PhysiologicProtein TransportReceptors, Cell SurfaceTight JunctionsZonula Occludens-1 ProteinConceptsCell-cell tensionAdherens junctionsActive myosin IIZO-1VE-cadherinBarrier formationEndothelial adherens junctionsJunctional recruitmentPrimary endothelial cellsCadherin complexActomyosin organizationCentral regulatorStress fibersInhibition of ROCKMyosin IIProtein ZO-1Tight junction protein ZO-1Cell migrationIntercellular junctionsP114RhoGEFMechanotransducersTight junctionsEndothelial junctionsEndothelial cellsTight junction disruption
2014
Up-regulation of Thrombospondin-2 in Akt1-null Mice Contributes to Compromised Tissue Repair Due to Abnormalities in Fibroblast Function*
Bancroft T, Bouaouina M, Roberts S, Lee M, Calderwood DA, Schwartz M, Simons M, Sessa WC, Kyriakides TR. Up-regulation of Thrombospondin-2 in Akt1-null Mice Contributes to Compromised Tissue Repair Due to Abnormalities in Fibroblast Function*. Journal Of Biological Chemistry 2014, 290: 409-422. PMID: 25389299, PMCID: PMC4281743, DOI: 10.1074/jbc.m114.618421.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementFibroblastsGene Expression RegulationGenetic Complementation TestIntegrin beta1MiceMice, KnockoutNeuropeptidesNitric Oxide Synthase Type IIIPrimary Cell CultureProto-Oncogene Proteins c-aktRac1 GTP-Binding ProteinRNA, Small InterferingSignal TransductionSkinThrombospondinsWound HealingWounds, NonpenetratingMechanotransduction of shear stress occurs through changes in VE-cadherin and PECAM-1 tension: Implications for cell migration
Conway DE, Schwartz MA. Mechanotransduction of shear stress occurs through changes in VE-cadherin and PECAM-1 tension: Implications for cell migration. Cell Adhesion & Migration 2014, 9: 335-339. PMID: 25482618, PMCID: PMC4955370, DOI: 10.4161/19336918.2014.968498.Peer-Reviewed Original Research
2013
Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension across VE-Cadherin and PECAM-1
Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA. Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension across VE-Cadherin and PECAM-1. Current Biology 2013, 23: 1024-1030. PMID: 23684974, PMCID: PMC3676707, DOI: 10.1016/j.cub.2013.04.049.Peer-Reviewed Original ResearchConceptsFluid shear stressVE-cadherinCell-cell junctionsPECAM-1Junctional tensionCytoskeletal remodelingVascular morphogenesisGene expressionComplex consistingCells triggersFlow-dependent vascular remodelingIon channelsFRET measurementsEndothelial cells triggersMechanical tensionNormal vascular functionTension sensorDetectable tensionEC responseStatic cultureJunctional receptorsRemodelingCytoskeletonMorphogenesisVascular remodelingDeconstructing Dimensionality
Schwartz MA, Chen CS. Deconstructing Dimensionality. Science 2013, 339: 402-404. PMID: 23349278, DOI: 10.1126/science.1233814.Peer-Reviewed Original ResearchConceptsStem cell nicheVivo cellular behaviorTwo-dimensional cultureCell nicheVivo functionGene expressionExtracellular matrix gelCellular behaviorTissue culture plasticCell behaviorDifferentiated functionsCell processesCulture plasticThree-dimensional environmentMatrix gelCellsTissue engineersMorphogenesisNicheBiologistsMajor advancesExpressionFunctionOnly minimal changesMaintenance
2011
Src and Caveolin-1 Reciprocally Regulate Metastasis via a Common Downstream Signaling Pathway in Bladder Cancer
Thomas S, Overdevest JB, Nitz MD, Williams PD, Owens CR, Sanchez-Carbayo M, Frierson HF, Schwartz MA, Theodorescu D. Src and Caveolin-1 Reciprocally Regulate Metastasis via a Common Downstream Signaling Pathway in Bladder Cancer. Cancer Research 2011, 71: 832-841. PMID: 21148751, PMCID: PMC4306590, DOI: 10.1158/0008-5472.can-10-0730.Peer-Reviewed Original ResearchConceptsActin stress fibersCav-1Active SrcStress fibersPhosphorylation of p190RhoGAPSrc protein levelsDownstream Signaling PathwaysCaveolin-1 expressionActivity of RhoACav-1 overexpressionTail vein inoculationRegulates MetastasisBladder cancerCommon downstreamHuman bladder cancer cellsPhosphorylated formSignaling pathwaysSrc levelsCell migrationBiochemical studiesLung metastasesMetastasis developmentSrc expressionBladder cancer cellsSrc
2010
Cell adhesion: integrating cytoskeletal dynamics and cellular tension
Parsons JT, Horwitz AR, Schwartz MA. Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nature Reviews Molecular Cell Biology 2010, 11: 633-643. PMID: 20729930, PMCID: PMC2992881, DOI: 10.1038/nrm2957.Peer-Reviewed Original ResearchConceptsRho GTPasesGuanine nucleotide exchange factorsNucleotide exchange factorsGTPase-activating proteinsMyosin II activityActin-myosin contractionCytoskeletal dynamicsActin cytoskeletonCellular tensionExchange factorRho proteinsProtein TyrAdhesion dynamicsMorphogenetic processesCell rearAdhesion sizeCell frontIntegrin clusteringActin polymerizationRho activationMyosin IIDownstream signalingFeedback loopCell migrationComplex feedback loopsMyosin II directly binds and inhibits Dbl family guanine nucleotide exchange factors: a possible link to Rho family GTPases
Lee CS, Choi CK, Shin EY, Schwartz MA, Kim EG. Myosin II directly binds and inhibits Dbl family guanine nucleotide exchange factors: a possible link to Rho family GTPases. Journal Of Cell Biology 2010, 190: 663-674. PMID: 20713598, PMCID: PMC2928003, DOI: 10.1083/jcb.201003057.Peer-Reviewed Original ResearchMeSH KeywordsActomyosinAnimalsBinding SitesCdc42 GTP-Binding ProteinCell AdhesionCell MovementEnzyme ActivationGuanine Nucleotide Exchange FactorsHumansJurkat CellsMiceMyosin Type IINIH 3T3 CellsPlatelet-Derived Growth FactorProtein BindingRac1 GTP-Binding ProteinRatsRecombinant Fusion ProteinsRho GTP-Binding ProteinsRho Guanine Nucleotide Exchange FactorsRNA, Small InterferingConceptsFocal complex formationDbl family guanineMyosin IIExchange factorFamily guanineATPase activityNonmuscle myosin IIComplex formationGEF activitySpatiotemporal regulationRho familyCdc42 GTPasesAdhesion dynamicsRho GTPasesCdc42 activationLamellipodial protrusionCell protrusionsActomyosin contractionGEFNIH3T3 fibroblastsFunctional linkCell migrationGTPasesCatalytic siteHomology modulesMeasuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature 2010, 466: 263-266. PMID: 20613844, PMCID: PMC2901888, DOI: 10.1038/nature09198.Peer-Reviewed Original ResearchConceptsFocal adhesionsFocal adhesion dynamicsMembrane cytoskeletal proteinsAdhesion dynamicsCell adhesion moleculeRegulatory mechanismsSpecific proteinsActin filamentsCell adhesionVinculinProteinMechanical tensionMechanical forcesRegulationPhysical forcesMolecular forcesAdhesionCellsVivoMechanotransductionPhysiologyNew biosensorFilamentsAbilityMigration
2008
Endogenous RhoG is dispensable for integrin-mediated cell spreading but contributes to Rac-independent migration
Meller J, Vidali L, Schwartz MA. Endogenous RhoG is dispensable for integrin-mediated cell spreading but contributes to Rac-independent migration. Journal Of Cell Science 2008, 121: 1981-1989. PMID: 18505794, PMCID: PMC2759683, DOI: 10.1242/jcs.025130.Peer-Reviewed Original Research
2006
Integrin signalling in directed cell migration
Moissoglu K, Schwartz MA. Integrin signalling in directed cell migration. Biology Of The Cell 2006, 98: 547-555. PMID: 16907663, DOI: 10.1042/bc20060025.Peer-Reviewed Original ResearchIntegrating Adhesion, Protrusion, and Contraction during Cell Migration
Schwartz MA, Horwitz AR. Integrating Adhesion, Protrusion, and Contraction during Cell Migration. Cell 2006, 125: 1223-1225. PMID: 16814706, DOI: 10.1016/j.cell.2006.06.015.Peer-Reviewed Original Research
2005
Integrin-dependent actomyosin contraction regulates epithelial cell scattering
de Rooij J, Kerstens A, Danuser G, Schwartz MA, Waterman-Storer CM. Integrin-dependent actomyosin contraction regulates epithelial cell scattering. Journal Of Cell Biology 2005, 171: 153-164. PMID: 16216928, PMCID: PMC2171213, DOI: 10.1083/jcb.200506152.Peer-Reviewed Original ResearchConceptsCell-cell junctionsEpithelial cell scatteringCell-cell adhesionCell scatteringHepatocyte growth factorE-cadherin functionMadin-Darby canine kidneyMyosin regulatory light chainExtracellular matrix proteinsTime-lapse imagingPossible cross talkCarcinoma cell invasionTraction forceRegulatory light chainIntegrin adhesionEpithelial-mesenchymal transitionActomyosin contractionMatrix proteinsCell invasionHigh traction forceMimic key aspectsCross talkSubstrate complianceGrowth factorCanine kidneyRho Proteins in Cell Migration and Metastasis
Titus B, Schwartz MA, Theodorescu D. Rho Proteins in Cell Migration and Metastasis. Critical Reviews In Eukaryotic Gene Expression 2005, 15: 103-114. DOI: 10.1615/critreveukargeneexpr.v15.i2.20.Peer-Reviewed Original ResearchRho Proteins in Cell Migration and Metastasis
Titus B, Schwartz M, Theodorescu D. Rho Proteins in Cell Migration and Metastasis. Critical Reviews In Eukaryotic Gene Expression 2005, 15: 103-114. PMID: 16022631, DOI: 10.1615/critreveukaryotgeneexpr.v15.i2.20.Peer-Reviewed Original Research
2004
VE-cadherin Links tRNA Synthetase Cytokine to Anti-angiogenic Function*
Tzima E, Reader JS, Irani-Tehrani M, Ewalt KL, Schwartz MA, Schimmel P. VE-cadherin Links tRNA Synthetase Cytokine to Anti-angiogenic Function*. Journal Of Biological Chemistry 2004, 280: 2405-2408. PMID: 15579907, DOI: 10.1074/jbc.c400431200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acyl-tRNA SynthetasesAngiogenesis InhibitorsAnimalsAntigens, CDAortaBlotting, WesternCadherinsCattleCell MovementCells, CulturedCytokinesEndothelium, VascularEnzyme ActivationExtracellular Signal-Regulated MAP KinasesGap JunctionsGreen Fluorescent ProteinsImmunoprecipitationMicroscopy, ConfocalMicroscopy, FluorescenceNeovascularization, PathologicProtein BindingRecombinant ProteinsSignal TransductionTryptophan-tRNA LigaseVascular Endothelial Growth Factor AConceptsT2-TrpRSRho Mediates the Shear-Enhancement of Endothelial Cell Migration and Traction Force Generation
Shiu YT, Li S, Marganski WA, Usami S, Schwartz MA, Wang YL, Dembo M, Chien S. Rho Mediates the Shear-Enhancement of Endothelial Cell Migration and Traction Force Generation. Biophysical Journal 2004, 86: 2558-2565. PMID: 15041692, PMCID: PMC1304103, DOI: 10.1016/s0006-3495(04)74311-8.Peer-Reviewed Original Research
2003
Cell Migration: Integrating Signals from Front to Back
Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. Cell Migration: Integrating Signals from Front to Back. Science 2003, 302: 1704-1709. PMID: 14657486, DOI: 10.1126/science.1092053.Peer-Reviewed Original Research