Spatial and temporal control of Rho GTPase functions
Moissoglu K, Schwartz MA. Spatial and temporal control of Rho GTPase functions. Cellular Logistics 2014, 4: e943618. PMID: 25610718, PMCID: PMC4279778, DOI: 10.4161/21592780.2014.943618.Peer-Reviewed Original ResearchCellular compartmentsRho GTPase biologyRho GTPases functionRho GTPase functionMultiple cellular compartmentsDifferent cellular compartmentsTemporal controlGTPase inactivationMembrane targetingGTPases functionGTPase functionCellular functionsRho GTPaseCell physiologyBiochemical regulationEffector activationMultiple regulatorsMultiple effectorsWealth of knowledgeSame cellsRegulationCompartmentsNano-scale dynamicsGTPaseMajor questionsRegulation of Rac1 translocation and activation by membrane domains and their boundaries
Moissoglu K, Kiessling V, Wan C, Hoffman BD, Norambuena A, Tamm LK, Schwartz MA. Regulation of Rac1 translocation and activation by membrane domains and their boundaries. Journal Of Cell Science 2014, 127: 2565-2576. PMID: 24695858, PMCID: PMC4038948, DOI: 10.1242/jcs.149088.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneFluorescence Resonance Energy TransferGTPase-Activating ProteinsHEK293 CellsHumansMembrane MicrodomainsMiceNIH 3T3 CellsProtein BindingProtein TransportRac1 GTP-Binding ProteinRho-Specific Guanine Nucleotide Dissociation InhibitorsSignal TransductionUnilamellar LiposomesConceptsFluorescence resonance energy transferMembrane domainsRac1 translocationGDP dissociation inhibitor proteinLiquid-ordered membrane domainsGTPase-activating proteinsNon-raft regionsNon-raft domainsBinding of Rac1Activation of Rac1Single-molecule analysisGTP loadingRho GTPasesLipid raftsRac1 localizationRho GTPaseInhibitor proteinResult of inactivationRac1Resonance energy transferFunctional studiesNovel mechanismLipid bilayersTranslocationRafts