Featured Publications
Pathogenic Autoreactive T and B Cells Cross-React with Mimotopes Expressed by a Common Human Gut Commensal to Trigger Autoimmunity
Ruff WE, Dehner C, Kim WJ, Pagovich O, Aguiar CL, Yu AT, Roth AS, Vieira SM, Kriegel C, Adeniyi O, Mulla MJ, Abrahams VM, Kwok WW, Nussinov R, Erkan D, Goodman AL, Kriegel MA. Pathogenic Autoreactive T and B Cells Cross-React with Mimotopes Expressed by a Common Human Gut Commensal to Trigger Autoimmunity. Cell Host & Microbe 2019, 26: 100-113.e8. PMID: 31227334, PMCID: PMC8194364, DOI: 10.1016/j.chom.2019.05.003.Peer-Reviewed Original ResearchConceptsAntiphospholipid syndromePathogenic monoclonal antibodyHuman autoimmune diseasesGut commensalsB-cell autoepitopesHuman gut commensalGPI IgGAPS patientsIgG titersOral gavageMemory TSusceptible miceAntigenic loadAutoimmune diseasesAutoimmune pathologyTrigger autoimmunityHuman autoimmunityGlycoprotein IGPI autoantibodiesAutoimmunityMonoclonal antibodiesCell clonesCross reactMimotopesAutoantibodies
2021
1504 The influence of dietary resistant starch content on the gut microbiota of patients with systemic lupus erythematosus and antiphospholipid syndrome
Kulyk I, Pereira M, Redanz S, Ruff W, Greiling T, Dehner C, Pagovich O, Ruiz D, Aguiar C, Erkan D, Kriegel M. 1504 The influence of dietary resistant starch content on the gut microbiota of patients with systemic lupus erythematosus and antiphospholipid syndrome. Lupus Science & Medicine 2021, 8: a63-a64. DOI: 10.1136/lupus-2021-lupus21century.87.Peer-Reviewed Original ResearchSystemic lupus erythematosusAntiphospholipid syndromeLupus erythematosusGut microbiotaErythematosusPatientsSyndromeDevelopment of a New International Antiphospholipid Syndrome Classification Criteria Phase I/II Report: Generation and Reduction of Candidate Criteria
Barbhaiya M, Zuily S, Ahmadzadeh Y, Amigo M, Avcin T, Bertolaccini M, Branch D, Jesus G, Devreese K, Frances C, Garcia D, Guillemin F, Levine S, Levy R, Lockshin M, Ortel T, Seshan S, Tektonidou M, Wahl D, Willis R, Naden R, Costenbader K, Erkan D, Agmon‐Levin N, Aguilar C, Alba P, Alpan O, Ambrozic A, Amoura Z, Andrade D, Andrade L, Appenzeller S, Esen B, Atsumi T, Berkun Y, Cabral A, Canaud G, Cervera R, Chen P, Chighizola C, Cimaz R, Cohen H, Costedoat‐Chalumeau N, Crowther M, Cuadrado M, de Groot P, de Moerloose P, Derksen R, Diz‐Kucukkaya R, Dörner T, Fortin P, Giannakopoulos B, Gómez‐Puerta J, Gonzalez E, Inanc M, Kenet G, Khamashta M, Kriegel M, Krilis S, Laskin C, Massicotte P, McCarty G, Meroni P, Mikdashi J, Myones B, Pengo V, Petri M, Roubey R, Sammaritano L, Sanna G, Sciascia S, Signorelli F, Soybilgic A, Tincani A, Woller S, Yelnik C. Development of a New International Antiphospholipid Syndrome Classification Criteria Phase I/II Report: Generation and Reduction of Candidate Criteria. Arthritis Care & Research 2021, 73: 1490-1501. PMID: 33253499, PMCID: PMC8966711, DOI: 10.1002/acr.24520.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsAntiphospholipid syndromeAPS classificationPhase IAPS classification criteriaCandidate criteriaPhase IIClassification criteriaItem reduction techniquesAmerican CollegeNominal group techniqueDelphi exerciseMultidisciplinary initiativeLaboratory domainHigher likelihoodRheumatologyCase collectionSystematic literature reviewPhysician scientistsPresent studyEuropean AllianceAggregate scoreLiterature reviewConsensus-based approach
2017
Autoantibody cross-reactivity with a microbial protein from a prevalent human gut commensal in antiphospholipid syndrome
ruff W, Roth A, Dehner C, Vieira S, Goodman A, Kriegel M. Autoantibody cross-reactivity with a microbial protein from a prevalent human gut commensal in antiphospholipid syndrome. The Journal Of Immunology 2017, 198: 58.4-58.4. DOI: 10.4049/jimmunol.198.supp.58.4.Peer-Reviewed Original ResearchAntiphospholipid syndromeAPS patientsGut commensalsBALB/cJ miceMajor B-cell epitopeGut commensal bacteriaAnti-B2GPI antibodiesB-cell epitopesInfectious triggerAutoantibody productionIgG autoantibodiesAntigenic sourceUnknown etiologyAutoantibody reactivityControl antibodyCell epitopesGut microbiotaCommensal bacteriaMajor autoantigenLow titersAutoantibodiesHuman stoolMonoclonal antibodiesVivo studiesPersistent stimulusAutoantigen‐specific T and B Cell Cross‐reactivity to a Gut Commensal in Antiphospholipid Syndrome
Dehner C, Ruff W, Vieira S, Goodman A, Kriegel M. Autoantigen‐specific T and B Cell Cross‐reactivity to a Gut Commensal in Antiphospholipid Syndrome. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.184.7.Peer-Reviewed Original ResearchBALB/c miceAdaptive immune systemAntiphospholipid syndromeB cellsC miceT cellsCell epitopesImmune systemPathogenic T cell epitopesTetramer-positive T cellsCD4 memory T cellsMemory T cellsTNF-α ILAdaptive immune responsesT cell clonesT cell epitopesDose-responsive mannerHuman commensal bacteriumB-cell epitopesEpitope-specific monoclonal antibodiesIL-21Patient PBMCsLymph nodesGPI antibodiesAntigenic challenge
2015
A common gut commensal as a cross-reactive candidate in antiphospholipid syndrome (HUM3P.250)
Dehner C, Ruff W, VIEIRA S, Goodman A, Kriegel M. A common gut commensal as a cross-reactive candidate in antiphospholipid syndrome (HUM3P.250). The Journal Of Immunology 2015, 194: 121.10-121.10. DOI: 10.4049/jimmunol.194.supp.121.10.Peer-Reviewed Original ResearchAntiphospholipid syndromeGut commensalsCD4 memory T cellsB-cell immunodominant epitopesWestern blottingIL-17 secretionMemory T cellsB cell responsesAdaptive immune responsesCommon gut commensalPatient plasma samplesAPS patientsIL-10IL-2IL-4T cellsImmune responseImmunodominant epitopesGut microbiotaTh1 clonesMajor autoantigenMolecular mimicryCell responsesPlasma samplesAntigenic potentialGut commensal dependence of autoreactivity and Th17 cells in systemic autoimmunity (MUC9P.740)
Manfredo Vieira S, Ruff W, Hiltensperger M, Yu A, Goodman A, Kriegel M. Gut commensal dependence of autoreactivity and Th17 cells in systemic autoimmunity (MUC9P.740). The Journal Of Immunology 2015, 194: 205.4-205.4. DOI: 10.4049/jimmunol.194.supp.205.4.Peer-Reviewed Original ResearchAntiphospholipid syndromeSegmented filamentous bacteriaSystemic autoimmunityGut microbiotaAntiphospholipid antibody productionRegulatory IL-10Gut microbiota compositionAntigen-specific effectsBroad-spectrum antibioticsAnaerobic gut microbiotaTh17 cellsIL-17Infectious triggerPathogenic autoantibodiesPulmonary emboliIL-10IL-21Myocardial infarctionIL-1BSplenocyte proliferationAntibody productionMicrobiota compositionImmune systemAutoimmunityMicrobiota
2014
Diet, microbiota and autoimmune diseases
Vieira S, Pagovich O, Kriegel M. Diet, microbiota and autoimmune diseases. Lupus 2014, 23: 518-526. PMID: 24763536, PMCID: PMC4009622, DOI: 10.1177/0961203313501401.Peer-Reviewed Original ResearchConceptsAutoimmune diseasesGut microbial communityGerm-free mouse modelDevelopment of autoimmunityDiet-derived metabolitesType 1 diabetesSeverity of diseaseLife-prolonging effectAdaptive immune systemAntiphospholipid syndromeAutoimmune modelSystemic lupusMultiple sclerosisGastrointestinal tractMurine modelMouse modelRodent modelsImmunomodulatory potentialCommensal bacteriaImmune systemCaloric restrictionGut microbiomeDietary changesLupusGut commensals