2010
Dynamin GTPase regulation is altered by PH domain mutations found in centronuclear myopathy patients
Kenniston JA, Lemmon MA. Dynamin GTPase regulation is altered by PH domain mutations found in centronuclear myopathy patients. The EMBO Journal 2010, 29: 3054-3067. PMID: 20700106, PMCID: PMC2944063, DOI: 10.1038/emboj.2010.187.Peer-Reviewed Original ResearchConceptsDynamin GTPase activityPH domain mutationsGTPase activityCNM mutationsConformational changesLarge GTPase dynaminGTP hydrolysis cycleC-terminal α-helixPleckstrin homology domainLow-resolution structureDomain mutationsReceptor-mediated endocytosisGTPase dynaminGTPase regulationPH domainScission functionCellular processesGTPase activationDynaminDomain rearrangementsVesicle invaginationGTPase rateCentronuclear myopathyHydrolysis cycleΑ-helix
2009
Auto‐inhibition of dynamin GTPase activity is regulated by PH domain interactions
Kenniston J, Lemmon M. Auto‐inhibition of dynamin GTPase activity is regulated by PH domain interactions. The FASEB Journal 2009, 23: 697.3-697.3. DOI: 10.1096/fasebj.23.1_supplement.697.3.Peer-Reviewed Original ResearchGTPase activityDynamin pleckstrin homology domainDynamin GTPase activityPleckstrin homology domainGTP hydrolysis ratePH domain interactionJane Coffin Childs Memorial FundReceptor-mediated endocytosisGTPase domainHomology domainDynamin GTPaseInteraction motifsGTP hydrolysisAbsence of lipidsConformational interplaySelective mutagenesisDomain interactionsGTPase rateVesicle interactionsDynaminScission eventsDistinct groupsGTPaseMutagenesisEndocytosis
1999
Dominant-negative inhibition of receptor-mediated endocytosis by a dynamin-1 mutant with a defective pleckstrin homology domain
Lee A, Frank D, Marks M, Lemmon M. Dominant-negative inhibition of receptor-mediated endocytosis by a dynamin-1 mutant with a defective pleckstrin homology domain. Current Biology 1999, 9: 261-265. PMID: 10074457, DOI: 10.1016/s0960-9822(99)80115-8.Peer-Reviewed Original ResearchConceptsPleckstrin homology domainPH domainReceptor-mediated endocytosisDynamin 1Homology domainEndocytic vesiclesPH domain bindsDynamin PH domainDominant negative inhibitorHigher-order oligomersDominant-negative inhibitionDynamin functionDomain bindsDynamin oligomersGTP bindingGTP hydrolysisGTPase activityPlasma membraneDynaminEndocytosisVesiclesPhosphoinositideBindsDomainMembrane
1998
Phosphatidylinositol-4,5-bisphosphate is required for endocytic coated vesicle formation
Jost M, Simpson F, Kavran J, Lemmon M, Schmid S. Phosphatidylinositol-4,5-bisphosphate is required for endocytic coated vesicle formation. Current Biology 1998, 8: 1399-1404. PMID: 9889104, DOI: 10.1016/s0960-9822(98)00022-0.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex 2Adaptor Proteins, Vesicular TransportBiotinCell MembraneClathrinEndocytosisEndosomesHumansIsoenzymesMutagenesisNeomycinNerve Tissue ProteinsPhosphatidylinositol 4,5-DiphosphatePhospholipase C deltaPhosphoproteinsProtein BindingTransferrinTumor Cells, CulturedType C PhospholipasesConceptsCoated vesicle formationEndocytic coated vesicle formationVesicle formationPleckstrin homology domainClathrin-coated vesiclesInvolvement of phosphatidylinositolReceptor-mediated endocytosisBind phosphatidylinositolGTPase dynaminAP2 complexProtein playersEndocytic motifEndocytic machineryHomology domainPH domainCoat assemblyInositol polyphosphateHigh-specificity probesGTPase activityInositol lipidsPhosphatidylinositolFirst direct evidenceDirect evidenceClathrinEndocytosis
1996
Identification of the Binding Site for Acidic Phospholipids on the PH Domain of Dynamin: Implications for Stimulation of GTPase Activity
Zheng J, Cahill S, Lemmon M, Fushman D, Schlessinger J, Cowburn D. Identification of the Binding Site for Acidic Phospholipids on the PH Domain of Dynamin: Implications for Stimulation of GTPase Activity. Journal Of Molecular Biology 1996, 255: 14-21. PMID: 8568861, DOI: 10.1006/jmbi.1996.0002.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesBlood ProteinsDynaminsGTP PhosphohydrolasesHumansKineticsMagnetic Resonance SpectroscopyModels, MolecularPhosphatidic AcidsPhosphatidylinositol 4,5-DiphosphatePhosphatidylinositol PhosphatesPhosphoproteinsProtein ConformationSequence Homology, Amino AcidSpectrometry, FluorescenceConceptsDynamin PH domainPH domainMembrane associationGTPase activityGuanine nucleotide exchange factorsNucleotide exchange factorsPleckstrin homology domainAcidic phospholipidsBinding of phospholipidsHomology domainExchange factorHuman dynaminGTP hydrolysisDynaminLipid head groupsLigand interactionsGTPaseBinding sitesPhosphatidylinositolSpecific sitesProteinPhospholipidsRelative affinityBindingDomain