1997
In vivo disassembly of free polyubiquitin chains by yeast Ubp14 modulates rates of protein degradation by the proteasome
Amerik A, Swaminathan S, Krantz B, Wilkinson K, Hochstrasser M. In vivo disassembly of free polyubiquitin chains by yeast Ubp14 modulates rates of protein degradation by the proteasome. The EMBO Journal 1997, 16: 4826-4838. PMID: 9305625, PMCID: PMC1170118, DOI: 10.1093/emboj/16.16.4826.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesCarbon-Nitrogen LyasesEndopeptidasesFungal ProteinsGene Expression Regulation, FungalGenes, FungalHumansImmunoblottingLyasesMolecular Sequence DataMutagenesis, Site-DirectedPeptide HydrolasesPhenotypeProteasome Endopeptidase ComplexProtein BindingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSubstrate SpecificityUbiquitinsConceptsUnanchored ubiquitin chainsUbiquitin chainsProtein degradationFree ubiquitin chainsUbiquitin-dependent proteolysisWild-type cellsActive site mutantsFree polyubiquitin chainsEukaryotic proteinsFunctional homologComplementation analysisPolyubiquitin chainsSteady-state levelsDeubiquitinating enzymeUbp14Site mutantsIsopeptidase TCellular proteasesYeast cellsProteasomeInhibition of degradationStriking accumulationProteolysisProteinCells
1990
In vivo degradation of a transcriptional regulator: The yeast α2 repressor
Hochstrasser M, Varshavsky A. In vivo degradation of a transcriptional regulator: The yeast α2 repressor. Cell 1990, 61: 697-708. PMID: 2111732, DOI: 10.1016/0092-8674(90)90481-s.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBeta-GalactosidaseFungal ProteinsGene Expression Regulation, FungalHalf-LifeMacromolecular SubstancesMolecular Sequence DataMutationProtein EngineeringProtein Processing, Post-TranslationalRecombinant Fusion ProteinsRepressor ProteinsSaccharomyces cerevisiaeTranscription FactorsConceptsYeast S. cerevisiaeTranscriptional regulatorsHeteromeric proteinsAlpha 2S. cerevisiaeDegradation signalRegulatory proteinsOligomeric proteinsSame proteinStructural domainsProteinMultiple functionsSubunitsRepressorDistinct mechanismsVivo concentrationsAdditional defectsCerevisiaeMutantsNovel typeDegradationRegulatorPathwayMetabolic instabilityVivo degradation