2019
The tumour microenvironment and immune milieu of cholangiocarcinoma
Fabris L, Perugorria M, Mertens J, Björkström NK, Cramer T, Lleo A, Solinas A, Sänger H, Lukacs‐Kornek V, Moncsek A, Siebenhüner A, Strazzabosco M. The tumour microenvironment and immune milieu of cholangiocarcinoma. Liver International 2019, 39: 63-78. PMID: 30907492, PMCID: PMC10878127, DOI: 10.1111/liv.14098.Peer-Reviewed Original ResearchConceptsTumor microenvironmentAbundant desmoplastic reactionCancer-associated fibroblastsHigh mortality rateTumor-promoting functionsEpithelial cancer cellsImmune milieuImmune cellsPancreatic cancerEffective therapyDesmoplastic reactionMortality rateCholangiocarcinomaInvasive abilityTherapeutic gainStromal cellsTumor cellsEndothelial cellsEpithelial counterpartsCancer cellsIntrahepatic variantCellular populationsMicroenvironmentCellsRecent studies
2013
Platelet‐derived growth factor‐D and Rho GTPases regulate recruitment of cancer‐associated fibroblasts in cholangiocarcinoma
Cadamuro M, Nardo G, Indraccolo S, Dall'Olmo L, Sambado L, Moserle L, Franceschet I, Colledan M, Massani M, Stecca T, Bassi N, Morton S, Spirli C, Fiorotto R, Fabris L, Strazzabosco M. Platelet‐derived growth factor‐D and Rho GTPases regulate recruitment of cancer‐associated fibroblasts in cholangiocarcinoma. Hepatology 2013, 58: 1042-1053. PMID: 23505219, PMCID: PMC3732815, DOI: 10.1002/hep.26384.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBenzamidesBile Duct NeoplasmsBile Ducts, IntrahepaticCell Line, TumorCell MovementCell ProliferationCells, CulturedCholangiocarcinomaEpithelial-Mesenchymal TransitionFibroblastsHeterograftsHumansImatinib MesylateIn Vitro TechniquesLymphokinesMaleMiceMice, SCIDPiperazinesPlatelet-Derived Growth FactorPyrimidinesRho GTP-Binding ProteinsSignal TransductionConceptsCancer-associated fibroblastsPlatelet-derived growth factorEpithelial-mesenchymal transitionCCA cellsSecretion of PDGFRole of PDGFGrowth factorAbundant stromal reactionAlpha-smooth muscle actinPDGF-D expressionNovel therapeutic approachesPotential therapeutic targetSmooth muscle actinCCA cell linesPDGF-D signalingFibroblast migrationC-Jun N-terminal kinaseEMT biomarkersImmunodeficient miceStromal reactionTherapeutic approachesStroma interactionsTherapeutic targetCholangiocarcinomaMesenchymal markersIsolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions
MASSANI M, STECCA T, FABRIS L, CARATOZZOLO E, RUFFOLO C, FURLANETTO A, MORTON S, CADAMURO M, STRAZZABOSCO M, BASSI N. Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions. Oncology Reports 2013, 30: 1143-1148. PMID: 23807641, DOI: 10.3892/or.2013.2568.Peer-Reviewed Original ResearchMeSH KeywordsBile Duct NeoplasmsBile Ducts, IntrahepaticBiomarkers, TumorCell CommunicationCholangiocarcinomaCoculture TechniquesEpithelial-Mesenchymal TransitionFibroblastsFlow CytometryFluorescent Antibody TechniqueHumansImmunoenzyme TechniquesImmunomagnetic SeparationNeoplasm GradingStromal CellsTumor Cells, CulturedConceptsHuman biliary epithelial cellsTumor-stroma interactionsCancer-associated fibroblastsStromal cellsOrganotypic co-culture modelPrimary culturesTumor cell originMesenchymal cell markersBiliary epithelial cellsCCA cell linesRat cholangiocarcinomaCo-culture modelDevastating malignancySurgical resectionBile ductPresent studySurgical specimensDesmoplastic reactionCholangiocarcinomaCell originHuman cholangiocarcinomaCell markersFluorescent immunocytochemistryEpithelial cellsCK7