2000
Ca2+‐activated Cl− channels can substitute for CFTR in stimulation of pancreatic duct bicarbonate secretion
ZSEMBERY Á, Strazzabosco M, Graf J. Ca2+‐activated Cl− channels can substitute for CFTR in stimulation of pancreatic duct bicarbonate secretion. The FASEB Journal 2000, 14: 2345-2356. PMID: 11053257, DOI: 10.1096/fj.99-0509com.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateBicarbonatesCalciumCesiumChloride ChannelsChloridesCyclic AMPCystic Fibrosis Transmembrane Conductance RegulatorGlyburideGlycerolHumansHydrogen-Ion ConcentrationMembrane PotentialsMutationPancreatic DuctsPatch-Clamp TechniquesPotassium ChannelsTumor Cells, Cultured
1998
Purinergic regulation of acid/base transport in human and rat biliary epithelial cell lines
Zsembery Á, Spirlì C, Granato A, LaRusso N, Okolicsanyi L, Crepaldi G, Strazzabosco M. Purinergic regulation of acid/base transport in human and rat biliary epithelial cell lines. Hepatology 1998, 28: 914-920. PMID: 9755225, DOI: 10.1002/hep.510280403.Peer-Reviewed Original ResearchConceptsCholangiocyte cell lineNHE activityPurinergic agonistsBiliary epithelial cell lineExtracellular ATPCell linesIntracellular cyclic adenosine monophosphate (cAMP) concentrationsBiliary HCO3- secretionRat cholangiocyte cell lineCyclic adenosine monophosphate concentrationsAdenosine receptor agonistsBiliary epithelial cellsMz-ChA-1 cellsAdenosine monophosphate concentrationsSynergistic stimulatory effectApical administrationEpithelial cell lineReceptor agonistP2Y2 receptorPurinergic receptorsBile volumePharmacological profileApical Cl- channelsBasolateral administrationHCO3- secretion
1997
New insights into cholangiocyte physiology
Strazzabosco M. New insights into cholangiocyte physiology. Journal Of Hepatology 1997, 27: 945-952. PMID: 9382988, DOI: 10.1016/s0168-8278(97)80338-8.Peer-Reviewed Original ResearchNa+‐dependent and ‐independent Cl−/HCO exchange mediate cellular HCO transport in cultured human intrahepatic bile duct cells
Strazzabosco M, Joplin R, Zsembery A, Wallace L, Spirli C, Fabris L, Granato A, Rossanese A, Poci C, Neuberger J, Okolicsanyi L, Crepaldi G. Na+‐dependent and ‐independent Cl−/HCO exchange mediate cellular HCO transport in cultured human intrahepatic bile duct cells. Hepatology 1997, 25: 976-985. PMID: 9096607, DOI: 10.1002/hep.510250431.Peer-Reviewed Original ResearchConceptsEpithelial membrane antigenIntracellular acid loadHuman cholangiocytesAcid loadIntrahepatic bile duct cellsFactor VIII-related antigenIntracellular cyclic adenosine monophosphate (cAMP) concentrationsBiliary HCO3- secretionPediatric liver transplantationAdministration of agentsCyclic adenosine monophosphate concentrationsHuman hepatocyte growth factorVIII-related antigenBile duct cellsNormal liver tissueBiliary epithelial cellsCl- channel inhibitorsHepatocyte growth factorAdenosine monophosphate concentrationsIntracellular cAMP concentrationLiver transplantationReduced graftAbsence of HCO3Biliary treeAcid loader
1994
Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes.
Benedetti A, Strazzabosco M, Ng O, Boyer J. Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 792-796. PMID: 8290601, PMCID: PMC43035, DOI: 10.1073/pnas.91.2.792.Peer-Reviewed Original ResearchConceptsApical targetingExchange activityRegulation of activityMembrane localizationCl-/HCO3Dibutyryl cAMPProtein kinase C agonistsRat hepatocytesProtein kinase C agonist phorbolEcto-ATPaseConfocal microscopyCanalicular domainC agonistsProteinVesiclesTargetingCanalicular localizationLocalizationCAMPHepatocyte coupletsHepatocytesActivityRegulationPhorbolColchicine
1991
Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH
Benedetti A, Strazzabosco M, Corasanti J, Haddad P, Graf J, Boyer J. Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH. American Journal Of Physiology 1991, 261: g512-g522. PMID: 1887897, DOI: 10.1152/ajpgi.1991.261.3.g512.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmmoniaAnimalsBariumBicarbonatesCarrier ProteinsCells, CulturedChloride-Bicarbonate AntiportersChloridesHydrogen-Ion ConcentrationIntracellular FluidKineticsLiverMaleRatsRats, Inbred StrainsConceptsAlkaline loadPH-sensitive dye BCECFMM H2DIDSPHi recoveryRegulation of intracellularRat hepatocyte monolayersAcid extrudersMM DIDSRat hepatocytesElectrophysiological techniquesDye BCECFHCO3- exchangerIsolated rat hepatocytesConstant pHoIntracellularMembrane potentialHepatocyte monolayersHepatocytesAcid loading