2007
Prolonged wakefulness induces experience-dependent synaptic plasticity in mouse hypocretin/orexin neurons
Rao Y, Liu ZW, Borok E, Rabenstein RL, Shanabrough M, Lu M, Picciotto MR, Horvath TL, Gao XB. Prolonged wakefulness induces experience-dependent synaptic plasticity in mouse hypocretin/orexin neurons. Journal Of Clinical Investigation 2007, 117: 4022-4033. PMID: 18060037, PMCID: PMC2104495, DOI: 10.1172/jci32829.Peer-Reviewed Original ResearchConceptsHypocretin/orexin neuronsLong-term potentiationOrexin neuronsGlutamatergic synapsesSynaptic plasticitySleep lossExperience-dependent synaptic plasticityDopamine D1 receptorsChronic sleep lossSleep-wake regulationModafinil treatmentLateral hypothalamusD1 receptorsSimilar potentiationBrain slicesNeuronal activityNeuronal circuitryDopamine systemNervous systemSynaptic strengthNeuronsPathological conditionsGentle handlingMiceWakefulness
2006
Galanin attenuates cyclic AMP regulatory element‐binding protein (CREB) phosphorylation induced by chronic morphine and naloxone challenge in Cath.a cells and primary striatal cultures
Hawes JJ, Narasimhaiah R, Picciotto MR. Galanin attenuates cyclic AMP regulatory element‐binding protein (CREB) phosphorylation induced by chronic morphine and naloxone challenge in Cath.a cells and primary striatal cultures. Journal Of Neurochemistry 2006, 96: 1160-1168. PMID: 16417577, DOI: 10.1111/j.1471-4159.2005.03613.x.Peer-Reviewed Original ResearchConceptsOpiate withdrawalAdditional potential therapeutic targetsChronic opiate administrationPotential therapeutic targetPrimary striatal culturesExtracellular signal-regulated kinase 1/2 phosphorylationElement-Binding Protein PhosphorylationCAMP regulatory element-binding proteinKinase 1/2 phosphorylationGalanin treatmentMorphine administrationChronic morphineGalanin receptorsNeuropeptide galaninOpiate administrationNaloxone challengeStriatal neuronsLocus coeruleusSomatic signsNucleus accumbensStriatal culturesRegulatory element-binding proteinTherapeutic targetElement-binding proteinMolecular alterations
2005
GalR1, but not GalR2 or GalR3, levels are regulated by galanin signaling in the locus coeruleus through a cyclic AMP‐dependent mechanism
Hawes JJ, Brunzell DH, Wynick D, Zachariou V, Picciotto MR. GalR1, but not GalR2 or GalR3, levels are regulated by galanin signaling in the locus coeruleus through a cyclic AMP‐dependent mechanism. Journal Of Neurochemistry 2005, 93: 1168-1176. PMID: 15934937, PMCID: PMC1352153, DOI: 10.1111/j.1471-4159.2005.03105.x.Peer-Reviewed Original ResearchConceptsCAMP-dependent mannerKnockout micePhysiological functionsCREB phosphorylationProtein levelsGALR1 expressionCell linesGalanin knockout miceMRNA levelsCAMP levelsMouse brainCyclic AMP-dependent mechanismGalR3Important rolePhosphorylationGalR1GalR2ProteinNegative feedbackMiceExpressionLevelsNucleusCATH
2001
Galanin receptor 1 gene expression is regulated by cyclic AMP through a CREB‐dependent mechanism
Zachariou V, Georgescu D, Kansal L, Merriam P, Picciotto M. Galanin receptor 1 gene expression is regulated by cyclic AMP through a CREB‐dependent mechanism. Journal Of Neurochemistry 2001, 76: 191-200. PMID: 11145992, DOI: 10.1046/j.1471-4159.2001.00018.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineCloning, MolecularCodon, InitiatorColforsinCyclic AMPCyclic AMP Response Element-Binding ProteinGene Expression RegulationMiceMolecular Sequence DataMutagenesis, Site-DirectedNerve Growth FactorNeuronsPromoter Regions, GeneticReceptors, GalaninReceptors, NeuropeptideRegulatory Sequences, Nucleic AcidSequence Homology, Nucleic AcidTetradecanoylphorbol AcetateTransfectionConceptsLocus coeruleusOpiate withdrawalGalanin binding sitesPeripheral nervous systemCREB-dependent mechanismG protein-coupled receptorsLevels of cAMPProtein-coupled receptorsOpiate analgesiaNeuropeptide galaninGalanin receptorsGalR1 mRNANervous systemPlace preferenceTranscription factor CREBForskolin effectGALR1 expressionGalR1 geneCAMP levelsCyclic AMPCell linesBasal activityCRE-like siteTransient transfection assaysReceptors
1988
Purification and characterization of PCPP‐260: A Purkinje cell‐enriched cyclic amp‐regulated membrane phosphoprotein of Mr 260,000
Weeks G, Picciotto M, Nairn A, Walaas S, Greengard P. Purification and characterization of PCPP‐260: A Purkinje cell‐enriched cyclic amp‐regulated membrane phosphoprotein of Mr 260,000. Synapse 1988, 2: 89-96. PMID: 2844000, DOI: 10.1002/syn.890020112.Peer-Reviewed Original ResearchConceptsCAMP-dependent protein kinaseMembrane proteinsProtein kinaseN-lauryl sarcosineIntegral membrane proteinsMajor tryptic phosphopeptidesPhosphoamino acid analysisTotal membrane proteinSodium dodecyl sulfate-polyacrylamide gel electrophoresisMembrane phosphoproteinDodecyl sulfate-polyacrylamide gel electrophoresisTryptic phosphopeptidesSulfate-polyacrylamide gel electrophoresisPossible functional roleProminent proteinsAlpha-methyl mannosideParticulate fractionMammalian cerebellumFunctional roleProteinPeptide mappingConcanavalin A-agaroseGel electrophoresisAcid analysisA-agarose