2011
FACS purification of immunolabeled cell types from adult rat brain
Guez-Barber D, Fanous S, Harvey BK, Zhang Y, Lehrmann E, Becker KG, Picciotto MR, Hope BT. FACS purification of immunolabeled cell types from adult rat brain. Journal Of Neuroscience Methods 2011, 203: 10-18. PMID: 21911005, PMCID: PMC3221768, DOI: 10.1016/j.jneumeth.2011.08.045.Peer-Reviewed Original ResearchConceptsFluorescence-activated cell sortingCell typesPromoter-driven reporter geneBrain tissueExtracellular proteinsFACS procedureReporter geneFACS purificationRat brainReal-time PCRMolecular analysisSorted cellsCell sortingAdult rat brainTime PCRIntact cell bodiesTransgenic miceMolecular alterationsNeuN antibodyQuantitative assayCell bodiesAvailable antibodiesBrainTissueGenes
1998
Pharmacological characterization of nicotinic receptor-stimulated GABA release from mouse brain synaptosomes.
Lu Y, Grady S, Marks M, Picciotto M, Changeux J, Collins A. Pharmacological characterization of nicotinic receptor-stimulated GABA release from mouse brain synaptosomes. Journal Of Pharmacology And Experimental Therapeutics 1998, 287: 648-57. PMID: 9808692.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidGABA releaseMouse brain synaptosomesBrain synaptosomesNicotinic agonistsNicotine bindingBeta2 null mutant miceNull mutant miceRecent electrophysiological studiesRodent brain tissueConcentration-dependent mannerAgonist inhibitionAlpha-BungarotoxinNeurochemical approachesPharmacological characterizationNM tetrodotoxinElectrophysiological studiesSame agonistBrain regionsBrain tissueMutant miceAgonistsEmax valuesBeta2 subunitMaximal release