2013
Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
Ramakrishnan A, Torok-Storb B, Pillai M. Primary Marrow-Derived Stromal Cells: Isolation and Manipulation. Methods In Molecular Biology 2013, 1035: 75-101. PMID: 23959984, PMCID: PMC3748384, DOI: 10.1007/978-1-62703-508-8_8.Peer-Reviewed Original ResearchConceptsMarrow stromal cellsStem cellsMSC populationsPrimary marrow stromal cellsMultipotent stem cellsImmortalized cell linesStromal cellsPrimary marrowMesenchymal lineagesCellular heterogeneityMesenchymal stem cellsHematopoietic cellsCell linesRare cellsCellsBone marrow mononuclear cellsMarrow mononuclear cellsBone marrow harvestLineagesBone marrowMarrow biopsyMononuclear cellsClinical transplantationMarrow harvestPopulation
2012
Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion
Yang X, Balakrishnan I, Torok-Storb B, Pillai M. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion. Advances In Hematology 2012, 2012: 142530. PMID: 22400029, PMCID: PMC3287024, DOI: 10.1155/2012/142530.Peer-Reviewed Original ResearchTotal body irradiationLethal total body irradiationWild-type C57/BL6 miceC57/BL6 miceEffects of MSCsPromising cellular therapyLethally Irradiated MiceSyngeneic MSCsCell infusionBody irradiationSingle infusionBL6 miceImmune histochemistryInfused MSCsTissue injuryLethal irradiationMarrow stromal cellsIrradiated miceInfusionCellular therapyStromal cellsRapid clearanceMarrow failureRT-PCRMice
2011
High Throughput Sequencing Following Cross-Linked Immune Precipitation (HITS-CLIP) of Argonaute (AGO) Identifies Mir-9 As a Regulator of MMP2 in the Marrow Microenvironment (ME)
Balakrishnan I, Yang X, Torok-Storb B, Hesselberth J, Pillai M. High Throughput Sequencing Following Cross-Linked Immune Precipitation (HITS-CLIP) of Argonaute (AGO) Identifies Mir-9 As a Regulator of MMP2 in the Marrow Microenvironment (ME). Blood 2011, 118: 2392. DOI: 10.1182/blood.v118.21.2392.2392.Peer-Reviewed Original ResearchGenome-wide approachesHITS-CLIPMiRNA-mRNA interactomeMiR-9Functional nichesNext-generation sequencingHuman umbilical vein endothelial cellsGene expressionMarrow microenvironmentNew genome-wide approachDirect bindingLuciferase assayMiRNA-based regulationPost-transcriptional regulationCross-species conservationSpecific functional nichesHigh-throughput sequencingMiRNA-mRNA interactionsSmall noncoding RNAsRegulation of hematopoiesisPutative binding sitesStromal cellsCobblestone areaSequence-specific fashionCross-linked RNA
2010
MiR-886-3p Down Regulates CXCL12 (SDF1) Expression in Human Marrow Stromal Cells
Pillai M, Yang X, Balakrishnan I, Bemis L, Torok-Storb B. MiR-886-3p Down Regulates CXCL12 (SDF1) Expression in Human Marrow Stromal Cells. PLOS ONE 2010, 5: e14304. PMID: 21179442, PMCID: PMC3001477, DOI: 10.1371/journal.pone.0014304.Peer-Reviewed Original Research
2009
Mir-886-3p Contributes to the Regulation of the hematopoietic microenvironment by down-Regulating SDF-1α (CXCL12).
Pillai M, Yang X, Iwata M, Bemis L, Torok-Storb B. Mir-886-3p Contributes to the Regulation of the hematopoietic microenvironment by down-Regulating SDF-1α (CXCL12). Blood 2009, 114: 561. DOI: 10.1182/blood.v114.22.561.561.Peer-Reviewed Original ResearchStem cell nicheCell nicheVivo cell-cell interactionsGene expressionExpression profilesQuantitative RT-PCRHematopoietic stem cell nicheHS27a cellsLong-term cultureStromal cellsCell-cell interactionsMiR‑886‑3pCell linesPrimary marrow stromal cellsStromal cell lineMiRNA expression profilesImmortalized cell linesSDF-1αGene productsMarrow stromal cellsRT-PCRUndifferentiated stateTranscript levelsMiRNA transfectionHematopoietic stem
2006
Reduced expression of inducible gelatinase B/matrix metalloproteinase-9 in monocytes from patients with myelodysplastic syndrome: correlation of inducible levels with the percentage of cytogenetically marked cells and with marrow cellularity
Iwata M, Pillai M, Ramakrishnan A, Hackman R, Deeg H, Opdenakker G, Torok-Storb B. Reduced expression of inducible gelatinase B/matrix metalloproteinase-9 in monocytes from patients with myelodysplastic syndrome: correlation of inducible levels with the percentage of cytogenetically marked cells and with marrow cellularity. Blood 2006, 109: 85-92. PMID: 16954500, PMCID: PMC1785081, DOI: 10.1182/blood-2006-05-020289.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBone MarrowCells, CulturedChemokinesCulture Media, ConditionedCytokinesEnzyme InductionFemaleFibroblastsHumansIn Situ Hybridization, FluorescenceLipopolysaccharide ReceptorsMaleMatrix Metalloproteinase 9Middle AgedMonocytesMyelodysplastic SyndromesRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStromal CellsConceptsMMP-9Myelodysplastic syndromeMarrow cellularityMonocyte populationsMatrix metalloproteinasesGelatinase B/matrix metalloproteinase-9Stromal cellsMCP-1/CCL2IL-8/CXCL8SDF-1/CXCL12Matrix metalloproteinase-9MMP-9 expressionMarker-positive cellsInducible levelsMDS patientsIL-6Healthy donorsMetalloproteinase-9Marrow stromal cellsG-CSFPatientsGM-CSFMonocytesStromal factorsM-CSF
2005
Inducible Levels of Gelatinase B/Matrix Metalloproteinase-9 Gene Expression in Monocytes Are Associated with Marrow Cellularity in Myelodysplastic Syndrome (MDS).
Iwata M, Pillai M, Deeg H, Opdenakker G, Torok-Storb B. Inducible Levels of Gelatinase B/Matrix Metalloproteinase-9 Gene Expression in Monocytes Are Associated with Marrow Cellularity in Myelodysplastic Syndrome (MDS). Blood 2005, 106: 1391. DOI: 10.1182/blood.v106.11.1391.1391.Peer-Reviewed Original ResearchMMP-9 gene expressionGene expressionInducible gene expressionStromal cellsMatrix metalloproteinase-9 gene expressionProtein expressionCritical regulatory moleculesMMP-9 proteinMatrix metalloproteinase-9MMP-9 protein secretionMyeloid cellsHuman matrix metalloproteinase 9Mature myeloid cellsStromal cell functionRegulatory moleculesErythroid lineageStromal signalsHematopoietic regulationProtein secretionReal-time PCRMMP-9 geneInducible levelsHS-5 stromal cellsCell functionGenes