2010
High Throughput Sequencing Following Cross-Linked Immune Precipitation (HITS-CLIP) of Argonaute (AGO) Identifies Mir-193a as a Regulator of Jagged1 In Marrow Stromal Cells.
Balakrishnan I, Yang X, Torok-Storb B, Hesselberth J, Pillai M. High Throughput Sequencing Following Cross-Linked Immune Precipitation (HITS-CLIP) of Argonaute (AGO) Identifies Mir-193a as a Regulator of Jagged1 In Marrow Stromal Cells. Blood 2010, 116: 3847. DOI: 10.1182/blood.v116.21.3847.3847.Peer-Reviewed Original ResearchHigh-throughput sequencingStem cell nicheStromal cell lineArgonaute proteinsHITS-CLIPMiR-193aThroughput sequencingMRNA interactionsCell nicheCell linesSmall non-coding RNAsMarrow microenvironmentRNA-protein complexesMiRNA-based regulationMarrow stromal cell lineCross-link RNAPost-transcriptional regulationHematopoietic stem cell nicheHS27a cellsMajority of mRNAsMiRNA-mRNA interactionsUCSC Genome BrowserDirect interactionNon-coding RNAsLong-term culture
2009
Mir-886-3p Contributes to the Regulation of the hematopoietic microenvironment by down-Regulating SDF-1α (CXCL12).
Pillai M, Yang X, Iwata M, Bemis L, Torok-Storb B. Mir-886-3p Contributes to the Regulation of the hematopoietic microenvironment by down-Regulating SDF-1α (CXCL12). Blood 2009, 114: 561. DOI: 10.1182/blood.v114.22.561.561.Peer-Reviewed Original ResearchStem cell nicheCell nicheVivo cell-cell interactionsGene expressionExpression profilesQuantitative RT-PCRHematopoietic stem cell nicheHS27a cellsLong-term cultureStromal cellsCell-cell interactionsMiR‑886‑3pCell linesPrimary marrow stromal cellsStromal cell lineMiRNA expression profilesImmortalized cell linesSDF-1αGene productsMarrow stromal cellsRT-PCRUndifferentiated stateTranscript levelsMiRNA transfectionHematopoietic stem