2011
High Throughput Sequencing Following Cross-Linked Immune Precipitation (HITS-CLIP) of Argonaute (AGO) Identifies Mir-9 As a Regulator of MMP2 in the Marrow Microenvironment (ME)
Balakrishnan I, Yang X, Torok-Storb B, Hesselberth J, Pillai M. High Throughput Sequencing Following Cross-Linked Immune Precipitation (HITS-CLIP) of Argonaute (AGO) Identifies Mir-9 As a Regulator of MMP2 in the Marrow Microenvironment (ME). Blood 2011, 118: 2392. DOI: 10.1182/blood.v118.21.2392.2392.Peer-Reviewed Original ResearchGenome-wide approachesHITS-CLIPMiRNA-mRNA interactomeMiR-9Functional nichesNext-generation sequencingHuman umbilical vein endothelial cellsGene expressionMarrow microenvironmentNew genome-wide approachDirect bindingLuciferase assayMiRNA-based regulationPost-transcriptional regulationCross-species conservationSpecific functional nichesHigh-throughput sequencingMiRNA-mRNA interactionsSmall noncoding RNAsRegulation of hematopoiesisPutative binding sitesStromal cellsCobblestone areaSequence-specific fashionCross-linked RNA
2009
Mir-886-3p Contributes to the Regulation of the hematopoietic microenvironment by down-Regulating SDF-1α (CXCL12).
Pillai M, Yang X, Iwata M, Bemis L, Torok-Storb B. Mir-886-3p Contributes to the Regulation of the hematopoietic microenvironment by down-Regulating SDF-1α (CXCL12). Blood 2009, 114: 561. DOI: 10.1182/blood.v114.22.561.561.Peer-Reviewed Original ResearchStem cell nicheCell nicheVivo cell-cell interactionsGene expressionExpression profilesQuantitative RT-PCRHematopoietic stem cell nicheHS27a cellsLong-term cultureStromal cellsCell-cell interactionsMiR‑886‑3pCell linesPrimary marrow stromal cellsStromal cell lineMiRNA expression profilesImmortalized cell linesSDF-1αGene productsMarrow stromal cellsRT-PCRUndifferentiated stateTranscript levelsMiRNA transfectionHematopoietic stem
2005
Inducible Levels of Gelatinase B/Matrix Metalloproteinase-9 Gene Expression in Monocytes Are Associated with Marrow Cellularity in Myelodysplastic Syndrome (MDS).
Iwata M, Pillai M, Deeg H, Opdenakker G, Torok-Storb B. Inducible Levels of Gelatinase B/Matrix Metalloproteinase-9 Gene Expression in Monocytes Are Associated with Marrow Cellularity in Myelodysplastic Syndrome (MDS). Blood 2005, 106: 1391. DOI: 10.1182/blood.v106.11.1391.1391.Peer-Reviewed Original ResearchMMP-9 gene expressionGene expressionInducible gene expressionStromal cellsMatrix metalloproteinase-9 gene expressionProtein expressionCritical regulatory moleculesMMP-9 proteinMatrix metalloproteinase-9MMP-9 protein secretionMyeloid cellsHuman matrix metalloproteinase 9Mature myeloid cellsStromal cell functionRegulatory moleculesErythroid lineageStromal signalsHematopoietic regulationProtein secretionReal-time PCRMMP-9 geneInducible levelsHS-5 stromal cellsCell functionGenes