2006
Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding
Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB. Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding. The Journal Of Immunology 2006, 177: 3930-3938. PMID: 16951356, DOI: 10.4049/jimmunol.177.6.3930.Peer-Reviewed Original ResearchConceptsMHC class IClass IT cellsT cell responsesEffective immune responseT cell recognitionT cell hybridomasT cell membraneImmune responseCoreceptor functionCell responsesCell hybridomasInfected cellsCell recognitionIdentified mutationsCD8alphaCoreceptorCD8betaCDR3 loopsCellsEnhanced bindingCD8ImmunotherapyCD8alphabetaCD8β
2000
Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
Kim S, Devine L, Angevine M, DeMars R, Kavathas P. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. The Journal Of Immunology 2000, 165: 7285-7292. PMID: 11120863, DOI: 10.4049/jimmunol.165.12.7285.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsCells, CulturedChlamydia trachomatisCytotoxicity Tests, ImmunologicEpitopes, T-LymphocyteFemaleHematopoietic Stem CellsHLA-A2 AntigenHLA-B AntigensHumansImmunomagnetic SeparationLymphocyte CountLymphogranuloma VenereumMalePorinsProtein BindingT-Lymphocytes, CytotoxicConceptsHLA class ITetramer-binding T cellsCTL responsesT cellsPeripheral bloodClass IPeptide-specific T cellsHLA-A2 tetramersPeptide-pulsed targetsGenital tract infectionElicit CTL responsesMajor outer membrane proteinTetramer analysisTract infectionsPeptide stimulationImmunization trialsCytolytic activityChlamydia trachomatisUninfected individualsVivo inductionInfected individualsEpithelial cellsBloodBulk culturesStimulationHuman CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer
Devine L, Kieffer L, Aitken V, Kavathas P. Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer. The Journal Of Immunology 2000, 164: 833-838. PMID: 10623829, DOI: 10.4049/jimmunol.164.2.833.Peer-Reviewed Original ResearchConceptsChimeric proteinIg domainsCell-cell adhesion assaysBeta-beta dimersCD8 alphaForms of CD8CD8 betaTyrosine kinase p56lckCOS-7 cellsMHC class IDisulfide-linked homodimerCD8 alpha alpha homodimerCysteine residuesKinase p56lckCD8 alpha alphaAlpha alpha homodimerLack of expressionAlpha polypeptide chainPolypeptide chainMature T cellsCell surfaceClass IBeta-IgBeta betaAbility of CD8
1999
Molecular analysis of protein interactions mediating the function of the cell surface protein CD8
Devine L, Kavathas P. Molecular analysis of protein interactions mediating the function of the cell surface protein CD8. Immunologic Research 1999, 19: 201-210. PMID: 10493174, DOI: 10.1007/bf02786488.Peer-Reviewed Original ResearchConceptsProtein interactionsForms of CD8Such protein interactionsTyrosine kinase p56lckDisulfide-linked homodimerCell surface glycoproteinMolecular detailsKinase p56lckMutational analysisMolecular analysisFunctional differencesCoreceptor CD8ProteinHistocompatibility complex class IAffinity measurementsMajor histocompatibility complex class IComplex class ICellsP56lckHomodimerHeterodimersCrystal structureClass IGlycoproteinInteractionOrientation of the Ig domains of CD8 alpha beta relative to MHC class I.
Devine L, Sun J, Barr M, Kavathas P. Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. The Journal Of Immunology 1999, 162: 846-51. PMID: 9916707, DOI: 10.4049/jimmunol.162.2.846.Peer-Reviewed Original Research