1992
Parathyroid hormone-related protein. Evidence for secretion of a novel mid-region fragment by three different cell types.
Soifer NE, Dee KE, Insogna KL, Burtis WJ, Matovcik LM, Wu TL, Milstone LM, Broadus AE, Philbrick WM, Stewart AF. Parathyroid hormone-related protein. Evidence for secretion of a novel mid-region fragment by three different cell types. Journal Of Biological Chemistry 1992, 267: 18236-18243. PMID: 1517251, DOI: 10.1016/s0021-9258(19)37178-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarcinoma, Renal CellCell LineElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueHumansImmunohistochemistryKidney NeoplasmsKineticsMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProtein BiosynthesisProteinsTransfectionConceptsMid-region fragmentCell typesSecretory formBasic amino acid motifHormone-related proteinAmino acid motifsImportant biological functionsAmino acid sequenceExtensive post-translational processingPost-translational processingDifferent cell typesHuman renal carcinoma cellsInterspecies conservationSodium dodecyl sulfate-polyacrylamide gel electrophoresis analysisRenal carcinoma cellsAcid motifGel electrophoresis analysisAcid sequenceBiological functionsParathyroid hormone-related proteinRegion-specific immunoassaysRat insulinoma cellsCell extractsCleavage siteAmino acidsHuman dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen.
Smith L, Wertelecki W, Milstone L, Petty E, Seashore M, Braverman I, Jenkins T, Byers P. Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen. American Journal Of Human Genetics 1992, 51: 235-44. PMID: 1642226, PMCID: PMC1682688.Peer-Reviewed Original ResearchConceptsAmino-terminal propeptideFragile skinType IConnective tissue disordersEhlers-Danlos syndromeLax jointsType VIIAffected animalsSkinChildrenCultured fibroblastsNormal cellsEhlers-Danlos syndrome type VIIPro alphaDisordersPropeptideAlphaDermatosparaxisProteolytic processingSepsisN-proteinaseCellsSyndromeCollagen fibrils
1991
Quantitation of cross-linked protein: An alternative to counting cornified envelopes as an index of keratinocyte differentiation
Hough-Monroe L, Milstone L. Quantitation of cross-linked protein: An alternative to counting cornified envelopes as an index of keratinocyte differentiation. Analytical Biochemistry 1991, 199: 25-28. PMID: 1807158, DOI: 10.1016/0003-2697(91)90264-t.Peer-Reviewed Original ResearchCell DifferentiationCross-Linking ReagentsCulture TechniquesElectrophoresis, Polyacrylamide GelHumansImmunoblottingKeratinocytesMaleMembrane ProteinsSkinGlycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*
WU T, SOIFER N, BURTIS W, MILSTONE L, STEWART A. Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*. The Journal Of Clinical Endocrinology & Metabolism 1991, 73: 1002-1007. PMID: 1939513, DOI: 10.1210/jcem-73-5-1002.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEpidermisGlycosylationHumansInfant, NewbornKeratinocytesMaleParathyroid Hormone-Related ProteinProtein Processing, Post-TranslationalProteinsRecombinant Proteins
1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CountChick EmbryoCrystallinsCulture TechniquesDNAElectrophoresis, Polyacrylamide GelEpithelial CellsEpitheliumEye ProteinsGenesInsulinLens, CrystallineNucleic Acid HybridizationRNA, MessengerValineConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture