2016
The X-Ray Crystal Structure of the Keratin 1-Keratin 10 Helix 2B Heterodimer Reveals Molecular Surface Properties and Biochemical Insights into Human Skin Disease
Bunick CG, Milstone LM. The X-Ray Crystal Structure of the Keratin 1-Keratin 10 Helix 2B Heterodimer Reveals Molecular Surface Properties and Biochemical Insights into Human Skin Disease. Journal Of Investigative Dermatology 2016, 137: 142-150. PMID: 27595935, PMCID: PMC5514376, DOI: 10.1016/j.jid.2016.08.018.Peer-Reviewed Original Research
2015
Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27
Bunick CG, Presland RB, Lawrence OT, Pearton DJ, Milstone LM, Steitz TA. Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27. Journal Of Investigative Dermatology 2015, 135: 1801-1809. PMID: 25760235, PMCID: PMC4466033, DOI: 10.1038/jid.2015.102.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAnnexin A2Biomarkers, TumorCells, CulturedCrystallizationEpidermal CellsEpidermisExoribonucleasesFilaggrin ProteinsHSP27 Heat-Shock ProteinsHumansIntermediate Filament ProteinsKeratinocytesProtein BindingProtein TransportS100 ProteinsSensitivity and SpecificitySpectrometry, FluorescenceConceptsÅ resolution crystal structureProtein-protein interactionsHuman profilaggrinCalcium-binding domainKeratinocyte terminal differentiationMolecular functionsProtein interactionsTerminal domainShock protein 27Cell envelopeIdentification of targetsN-terminusMolecular approachesTerminal differentiationNormal epidermal barrierHydrophobic pocketSpecific functionsAnnexin IIStable dimerMolecular interfaceProtein 27Proteolytic productsProfilaggrinProteinCrystal structure
2004
Epidermal desquamation
Milstone LM. Epidermal desquamation. Journal Of Dermatological Science 2004, 36: 131-140. PMID: 15541634, DOI: 10.1016/j.jdermsci.2004.05.004.Commentaries, Editorials and Letters
2001
Photodegraded Nifedipine Stimulates Uptake and Retention of Iron in Human Epidermal Keratinocytes
Gruen A, Zhou J, Milstone L, Morton K. Photodegraded Nifedipine Stimulates Uptake and Retention of Iron in Human Epidermal Keratinocytes. Journal Of Investigative Dermatology 2001, 116: 774-777. PMID: 11348469, DOI: 10.1046/j.1523-1747.2001.01318.x.Peer-Reviewed Original ResearchConceptsHuman epidermal keratinocytesEpidermal keratinocytesD washout periodNifedipine treatmentDaily treatmentWashout periodNifedipineRetention of ironEpidermal desquamationIron uptakeKeratinocytesKeratinocyte monolayersSerum proteinsRelevant concentrationsToxic amountsEpidermal culturesTreatmentErythroid cells
1999
Growth and differentiation regulate CD44 expression on human keratinocytes
Zhou J, Haggerty J, Milstone L. Growth and differentiation regulate CD44 expression on human keratinocytes. In Vitro Cellular & Developmental Biology - Animal 1999, 35: 228-235. PMID: 10478803, DOI: 10.1007/s11626-999-0031-7.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationCell DivisionCells, CulturedEpidermal CellsEpidermisHumansHyaluronan ReceptorsKeratinocytesProteoglycansConceptsCD44 expressionBasal keratinocytesChondroitin sulfate proteoglycan formKeratan sulfate proteoglycanConfluent culturesPreconfluent monolayersDifferentiated cellsHyaluronan receptorWestern immunoblotCD44 proteinHuman keratinocytesCD44 familyKeratinocytesPreconfluent culturesSulfate proteoglycanDifferentiated keratinocytesCD44Core proteinSplice variants
1996
CD44 Expression on Epidermal Melanocytes
Herbold K, Zhou J, Haggerty J, Milstone L. CD44 Expression on Epidermal Melanocytes. Journal Of Investigative Dermatology 1996, 106: 1230-1235. PMID: 8752662, DOI: 10.1111/1523-1747.ep12348871.Peer-Reviewed Original Research
1991
Immunohistochemical Localization of Parathyroid Hormone-Related Protein (PTHRP) In Normal Human Skin
Atillasoy E, Burtis W, Milstone L. Immunohistochemical Localization of Parathyroid Hormone-Related Protein (PTHRP) In Normal Human Skin. Journal Of Investigative Dermatology 1991, 96: 277-280. PMID: 1991989, DOI: 10.1111/1523-1747.ep12464480.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related peptideNormal human skinHormone-related peptideHuman keratinocytesHuman skinParathyroid hormoneSquamous differentiationAffinity-purified polyclonal antibodiesNumber of autocrineImmunohistochemical localizationParacrine functionEpithelial cellsViable portionKeratinocytesSkinPolyclonal antibodiesRecent studiesPeptidesAutocrineHormoneAntibodies
1990
Measurement of retinoid effects on epidermal renewal
Milstone L. Measurement of retinoid effects on epidermal renewal. Methods In Enzymology 1990, 190: 76-81. PMID: 2087197, DOI: 10.1016/0076-6879(90)90011-o.Chapters
1989
Dipyridamole Potentiates the Growth-Inhibitory Action of Methotrexate and 5-Fluorouracil in Human Keratinocytes In Vitro
Schwartz P, Milstone L. Dipyridamole Potentiates the Growth-Inhibitory Action of Methotrexate and 5-Fluorouracil in Human Keratinocytes In Vitro. Journal Of Investigative Dermatology 1989, 93: 523-527. PMID: 2476512, DOI: 10.1111/1523-1747.ep12284073.Peer-Reviewed Original Research
1988
Effector Functions of Epidermal Keratinocytes
MILSTONE L. Effector Functions of Epidermal Keratinocytes. Annals Of The New York Academy Of Sciences 1988, 548: 1-3. PMID: 2470284, DOI: 10.1111/j.1749-6632.1988.tb18787.x.ChaptersLocal and Systemic Implications of Thymidine Catabolism by Human Keratinocytesa
SCHWARTZ P, REUVENI H, MILSTONE L. Local and Systemic Implications of Thymidine Catabolism by Human Keratinocytesa. Annals Of The New York Academy Of Sciences 1988, 548: 115-124. PMID: 2470288, DOI: 10.1111/j.1749-6632.1988.tb18798.x.ChaptersTwo distinct tumor-derived, parathyroid hormone-like peptides result from alternative ribonucleic acid splicing.
Mangin M, Ikeda K, Dreyer B, Milstone L, Broadus A. Two distinct tumor-derived, parathyroid hormone-like peptides result from alternative ribonucleic acid splicing. Endocrinology 1988, 2: 1049-55. PMID: 2464747, DOI: 10.1210/mend-2-11-1049.Peer-Reviewed Original Research
1986
A Parathyroid Hormone-Like Protein from Cultured Human Keratinocytes
Merendino JJ, Insogna KL, Milstone LM, Broadus AE, Stewart AF. A Parathyroid Hormone-Like Protein from Cultured Human Keratinocytes. Science 1986, 231: 388-390. PMID: 2417317, DOI: 10.1126/science.2417317.Peer-Reviewed Original ResearchConceptsHumoral hypercalcemiaParathyroid hormoneHuman keratinocytesParathyroid hormone receptor agonistsParathyroid hormone-like proteinSquamous epithelial originExtracts of tumorsHormone receptor agonistsHormone-like proteinHormone-like factorsAdenylate cyclase activityRat osteosarcoma cellsAdenylate cyclase productionNative parathyroid hormoneCultured human keratinocytesNormal human keratinocytesReceptor agonistMalignant tumorsCyclic adenosine monophosphateHypercalcemiaEpithelial originKeratinocyte-conditioned mediumMalignancyCyclase activityCyclase production
1984
Epidermal Cell Confluence and Implications for a Two-Step Mechanism of Wound Closure
Stenn K, Milstone L. Epidermal Cell Confluence and Implications for a Two-Step Mechanism of Wound Closure. Journal Of Investigative Dermatology 1984, 83: 445-447. PMID: 6209344, DOI: 10.1111/1523-1747.ep12273552.Peer-Reviewed Original Research