2015
Frequent somatic reversion of KRT1 mutations in ichthyosis with confetti
Choate KA, Lu Y, Zhou J, Elias PM, Zaidi S, Paller AS, Farhi A, Nelson-Williams C, Crumrine D, Milstone LM, Lifton RP. Frequent somatic reversion of KRT1 mutations in ichthyosis with confetti. Journal Of Clinical Investigation 2015, 125: 1703-1707. PMID: 25774499, PMCID: PMC4396494, DOI: 10.1172/jci64415.Peer-Reviewed Original ResearchAdultAge of OnsetAmino Acid SequenceCell Line, TumorCell NucleusChildChild, PreschoolChromosomes, Human, Pair 12CytoskeletonFrameshift MutationHumansIchthyosisIntermediate FilamentsKeratin-1KeratinocytesLoss of HeterozygosityMaleMolecular Sequence DataMosaicismPhenotypePolymorphism, Single NucleotideProtein TransportTransfectionCrystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27
Bunick CG, Presland RB, Lawrence OT, Pearton DJ, Milstone LM, Steitz TA. Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27. Journal Of Investigative Dermatology 2015, 135: 1801-1809. PMID: 25760235, PMCID: PMC4466033, DOI: 10.1038/jid.2015.102.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAnnexin A2Biomarkers, TumorCells, CulturedCrystallizationEpidermal CellsEpidermisExoribonucleasesFilaggrin ProteinsHSP27 Heat-Shock ProteinsHumansIntermediate Filament ProteinsKeratinocytesProtein BindingProtein TransportS100 ProteinsSensitivity and SpecificitySpectrometry, FluorescenceConceptsÅ resolution crystal structureProtein-protein interactionsHuman profilaggrinCalcium-binding domainKeratinocyte terminal differentiationMolecular functionsProtein interactionsTerminal domainShock protein 27Cell envelopeIdentification of targetsN-terminusMolecular approachesTerminal differentiationNormal epidermal barrierHydrophobic pocketSpecific functionsAnnexin IIStable dimerMolecular interfaceProtein 27Proteolytic productsProfilaggrinProteinCrystal structure
2012
Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays
Lara MF, González-González E, Speaker TJ, Hickerson RP, Leake D, Milstone LM, Contag CH, Kaspar RL. Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays. Human Gene Therapy 2012, 23: 816-823. PMID: 22480249, PMCID: PMC3413896, DOI: 10.1089/hum.2011.211.Peer-Reviewed Original ResearchConceptsTransgenic mouse modelInhibition of CD44CD44-specific antibodiesCD44 mRNA levelsDissolvable microneedle arraysGene expressionMouse modelXenograft modelSkin disordersCD44 gene expressionHuman skin modelTarget cellsMRNA levelsHuman skin equivalentsKeratinocyte membraneTreatmentInhibitionStratum corneumTarget gene expressionSiRNASkin equivalentsEpidermal equivalentsMicroneedle arraysSkin modelExpression
2011
Visualization of plasmid delivery to keratinocytes in mouse and human epidermis
González-González E, Kim YC, Speaker TJ, Hickerson RP, Spitler R, Birchall JC, Lara MF, Hu RH, Liang Y, Kirkiles-Smith N, Prausnitz MR, Milstone LM, Contag CH, Kaspar RL. Visualization of plasmid delivery to keratinocytes in mouse and human epidermis. Scientific Reports 2011, 1: 158. PMID: 22355673, PMCID: PMC3240989, DOI: 10.1038/srep00158.Peer-Reviewed Original ResearchConceptsIdeal target organHuman skin xenograftsAccessibility of skinSkin delivery technologiesNon-invasive intravital imagingIntradermal injectionSkin xenograftsClinical utilityTarget organsPatient-friendly deliveryMouse skinBioluminescence imagingSkin model systemIntravital imagingNoninvasive assayTransfection of cellsIntravital fluorescenceHuman epidermisIndividual keratinocytesKeratinocytesSkinNucleic acid-based therapeuticsMiceReporter plasmidDelivery technologies
2010
Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials
Hickerson RP, Leachman SA, Pho LN, Gonzalez-Gonzalez E, Smith FJ, McLean WH, Contag CH, Leake D, Milstone LM, Kaspar RL. Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials. Journal Of Investigative Dermatology 2010, 131: 1029-1036. PMID: 21191405, DOI: 10.1038/jid.2010.372.Peer-Reviewed Original ResearchConceptsClinical trialsPachyonychia congenitaEnd pointPhase 1b clinical trialClinical end pointsRare skin disorderMolecular end pointsTypes of trialsImmune surveillancePatient-derived keratinocytesSkin disordersSustained inhibitionTreatment resultsRNAi-based therapeuticsRNA interferenceMRNA levelsTrialsTotal RNAHuman skinKeratinocyte culturesMRNAAmplifiable mRNATherapeuticsInhibitorsInhibition
2007
Single-Nucleotide-Specific siRNA Targeting in a Dominant-Negative Skin Model
Hickerson RP, Smith FJ, Reeves RE, Contag CH, Leake D, Leachman SA, Milstone LM, McLean WH, Kaspar RL. Single-Nucleotide-Specific siRNA Targeting in a Dominant-Negative Skin Model. Journal Of Investigative Dermatology 2007, 128: 594-605. PMID: 17914454, DOI: 10.1038/sj.jid.5701060.Peer-Reviewed Original ResearchConceptsWild-type gene expressionBicistronic reporter constructFilament formationDominant-negative genetic disordersNumerous genetic disordersGenetic disordersKeratin filament formationRNA interferenceMutant formsReporter constructsGene expressionMutant mRNAFluorescent reportersMutant allelesCell culture modelSiRNAsSimultaneous expressionK6aK mutationPachyonychia congenitaExpressionCulture modelHuman keratinocytesSiRNASelective inhibition
2004
Long-Term Murine Keratinocyte Cultures Become Tetraploid, Yet Maintain the Ability to Stratify
Sanchez V, Zhou J, LaCivita D, Milstone L. Long-Term Murine Keratinocyte Cultures Become Tetraploid, Yet Maintain the Ability to Stratify. Journal Of Investigative Dermatology 2004, 123: 403-404. PMID: 15245442, DOI: 10.1111/j.0022-202x.2004.23218.x.Peer-Reviewed Original Research
2001
Photodegraded Nifedipine Stimulates Uptake and Retention of Iron in Human Epidermal Keratinocytes
Gruen A, Zhou J, Milstone L, Morton K. Photodegraded Nifedipine Stimulates Uptake and Retention of Iron in Human Epidermal Keratinocytes. Journal Of Investigative Dermatology 2001, 116: 774-777. PMID: 11348469, DOI: 10.1046/j.1523-1747.2001.01318.x.Peer-Reviewed Original ResearchConceptsHuman epidermal keratinocytesEpidermal keratinocytesD washout periodNifedipine treatmentDaily treatmentWashout periodNifedipineRetention of ironEpidermal desquamationIron uptakeKeratinocytesKeratinocyte monolayersSerum proteinsRelevant concentrationsToxic amountsEpidermal culturesTreatmentErythroid cells
1999
Expression and localization of thymidine phosphorylase/platelet‐derived endothelial cell growth factor in skin and cutaneous tumors
Asgari M, Haggerty J, McNiff J, Milstone L, Schwartz P. Expression and localization of thymidine phosphorylase/platelet‐derived endothelial cell growth factor in skin and cutaneous tumors. Journal Of Cutaneous Pathology 1999, 26: 287-294. PMID: 10472757, DOI: 10.1111/j.1600-0560.1999.tb01846.x.Peer-Reviewed Original ResearchConceptsPlatelet-derived endothelial cell growth factorThymidine phosphorylase/platelet-derived endothelial cell growth factorPD-ECGF expressionEndothelial cell growth factorCutaneous tumorsPD-ECGFCell carcinomaCell growth factorGrowth factorSquamous cell carcinomaMerkel cell carcinomaBasal cell carcinomaVariety of tumorsCell of originNuclei of keratinocytesFollicular infundibulumTumor degreeDifferentiation of keratinocytesEccrine poromaImmunohistochemical analysisPositive stainingIntradermal nevusNormal skinTumorsIntense stainingGrowth and differentiation regulate CD44 expression on human keratinocytes
Zhou J, Haggerty J, Milstone L. Growth and differentiation regulate CD44 expression on human keratinocytes. In Vitro Cellular & Developmental Biology - Animal 1999, 35: 228-235. PMID: 10478803, DOI: 10.1007/s11626-999-0031-7.Peer-Reviewed Original ResearchConceptsCD44 expressionBasal keratinocytesChondroitin sulfate proteoglycan formKeratan sulfate proteoglycanConfluent culturesPreconfluent monolayersDifferentiated cellsHyaluronan receptorWestern immunoblotCD44 proteinHuman keratinocytesCD44 familyKeratinocytesPreconfluent culturesSulfate proteoglycanDifferentiated keratinocytesCD44Core proteinSplice variants
1995
Keratinocytes differentiate in response to inhibitors of deoxyribonucleotide synthesis
Schwartz P, Barnett S, Milstone L. Keratinocytes differentiate in response to inhibitors of deoxyribonucleotide synthesis. Journal Of Dermatological Science 1995, 9: 129-135. PMID: 7772575, DOI: 10.1016/0923-1811(94)00370-t.Peer-Reviewed Original Research
1994
Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion
Milstone L, Hough-Monroe L, Kugelman L, Bender J, Haggerty J. Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion. Journal Of Cell Science 1994, 107: 3183-3190. PMID: 7699015, DOI: 10.1242/jcs.107.11.3183.Peer-Reviewed Original ResearchResponse of stratified cultures of human keratinocytes to disruption of proteoglycan synthesis by p‐nitrophenyl‐β‐D‐xylopyranoside
Haggerty J, Bretton R, Milstone L. Response of stratified cultures of human keratinocytes to disruption of proteoglycan synthesis by p‐nitrophenyl‐β‐D‐xylopyranoside. Journal Of Cellular Physiology 1994, 158: 39-46. PMID: 8263027, DOI: 10.1002/jcp.1041580106.Peer-Reviewed Original Research
1992
The Core Protein of Epican, a Heparan Sulfate Proteoglycan on Keratinocytes, Is an Alternative Form of CD44
Kugelman L, Ganguly S, Haggerty J, Weissman S, Milstone L. The Core Protein of Epican, a Heparan Sulfate Proteoglycan on Keratinocytes, Is an Alternative Form of CD44. Journal Of Investigative Dermatology 1992, 99: 887-891. PMID: 1281868, DOI: 10.1111/1523-1747.ep12614896.Peer-Reviewed Original ResearchConceptsHeparan sulfate proteoglycanCore proteinForm of CD44Human keratinocyte cDNA libraryDeduced protein sequenceAmino acid domainProximal extracellular domainKeratinocyte cDNA librarySulfate proteoglycanAcid domainCDNA libraryProtein sequencesGlycosylation sitesProteolysis siteExtracellular domainAdditional domainsAmino acidsProteinEpicanLeukocyte formCD44ProteoglycansDomainMonoclonal antibodiesKeratinocytesIdentification and Characterization of a Cell Surface Proteoglycan on Keratinocytes
Haggerty J, Bretton R, Milstone L. Identification and Characterization of a Cell Surface Proteoglycan on Keratinocytes. Journal Of Investigative Dermatology 1992, 99: 374-380. PMID: 1401993, DOI: 10.1111/1523-1747.ep12616087.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, SurfaceBlotting, WesternCell MembraneHumansKeratinocytesMaleProteoglycansConceptsCore proteinCell-cell contactIntercellular spacesCore protein moleculesCell surface proteoglycansCell extractsSurface proteoglycansEnzymatic deglycosylationProteinProtein moleculesSmall formsProteoglycansEpicanWestern immunoblotCD44 familyWestern blotSkin cellsHeparan sulfateHuman keratinocytesAnalysis of PTHRP binding and signal transduction mechanisms in benign and malignant squamous cells
Orloff J, Ganz M, Ribaudo A, Burtis W, Reiss M, Milstone L, Stewart A. Analysis of PTHRP binding and signal transduction mechanisms in benign and malignant squamous cells. American Journal Of Physiology 1992, 262: e599-e607. PMID: 1590371, DOI: 10.1152/ajpendo.1992.262.5.e599.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related proteinHuman epidermal keratinocytesIntracellular calciumImmunoreactive parathyroid hormone-related proteinLow-affinity binding sitesSqCC cellsDose-dependent transient increaseHuman parathyroid hormone-related proteinMalignant squamous cellsHormone-related proteinIntracellular calcium responseCyclic monophosphate responsePotential autocrine roleSquamous carcinoma cellsHigh-affinity receptorHalf maximal inhibitory concentration (IC50) valuesPTHrP concentrationsSquamous cellsPTH receptorIsoproterenol responseAutocrine roleCalcium responsePTHrP bindingMonophosphate responseInhibitory concentration valuesMethotrexate induces differentiation of human keratinocytes.
Schwartz P, Barnett S, Atillasoy E, Milstone L. Methotrexate induces differentiation of human keratinocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 594-598. PMID: 1731329, PMCID: PMC48285, DOI: 10.1073/pnas.89.2.594.Peer-Reviewed Original ResearchConceptsEffect of methotrexateAbility of methotrexateProliferation of keratinocytesProportion of cellsMarkers of differentiationHuman epidermal keratinocytesMethotrexateTherapeutic actionEpidermal keratinocytesMarked increaseHuman keratinocytesKeratinocytesEnvelope proteinSDS/PAGE profilesInvolucrin synthesisNormal cellsTerminal differentiationTissue homeostasisThymine deoxyribonucleotidesDifferentiationCellsPsoriasisCell morphologyReproducible changesDose
1991
Quantitation of cross-linked protein: An alternative to counting cornified envelopes as an index of keratinocyte differentiation
Hough-Monroe L, Milstone L. Quantitation of cross-linked protein: An alternative to counting cornified envelopes as an index of keratinocyte differentiation. Analytical Biochemistry 1991, 199: 25-28. PMID: 1807158, DOI: 10.1016/0003-2697(91)90264-t.Peer-Reviewed Original ResearchGlycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*
WU T, SOIFER N, BURTIS W, MILSTONE L, STEWART A. Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*. The Journal Of Clinical Endocrinology & Metabolism 1991, 73: 1002-1007. PMID: 1939513, DOI: 10.1210/jcem-73-5-1002.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEpidermisGlycosylationHumansInfant, NewbornKeratinocytesMaleParathyroid Hormone-Related ProteinProtein Processing, Post-TranslationalProteinsRecombinant ProteinsImmunoaffinity purification of parathyroid hormone‐related protein from bovine milk and human keratinocyte‐conditioned medium
Stewart A, Wu T, Insogna K, Milstone L, Burtis W. Immunoaffinity purification of parathyroid hormone‐related protein from bovine milk and human keratinocyte‐conditioned medium. Journal Of Bone And Mineral Research 1991, 6: 305-311. PMID: 2035357, DOI: 10.1002/jbmr.5650060313.Peer-Reviewed Original Research