2012
Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays
Lara MF, González-González E, Speaker TJ, Hickerson RP, Leake D, Milstone LM, Contag CH, Kaspar RL. Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays. Human Gene Therapy 2012, 23: 816-823. PMID: 22480249, PMCID: PMC3413896, DOI: 10.1089/hum.2011.211.Peer-Reviewed Original ResearchConceptsTransgenic mouse modelInhibition of CD44CD44-specific antibodiesCD44 mRNA levelsDissolvable microneedle arraysGene expressionMouse modelXenograft modelSkin disordersCD44 gene expressionHuman skin modelTarget cellsMRNA levelsHuman skin equivalentsKeratinocyte membraneTreatmentInhibitionStratum corneumTarget gene expressionSiRNASkin equivalentsEpidermal equivalentsMicroneedle arraysSkin modelExpression
2007
Single-Nucleotide-Specific siRNA Targeting in a Dominant-Negative Skin Model
Hickerson RP, Smith FJ, Reeves RE, Contag CH, Leake D, Leachman SA, Milstone LM, McLean WH, Kaspar RL. Single-Nucleotide-Specific siRNA Targeting in a Dominant-Negative Skin Model. Journal Of Investigative Dermatology 2007, 128: 594-605. PMID: 17914454, DOI: 10.1038/sj.jid.5701060.Peer-Reviewed Original ResearchConceptsWild-type gene expressionBicistronic reporter constructFilament formationDominant-negative genetic disordersNumerous genetic disordersGenetic disordersKeratin filament formationRNA interferenceMutant formsReporter constructsGene expressionMutant mRNAFluorescent reportersMutant allelesCell culture modelSiRNAsSimultaneous expressionK6aK mutationPachyonychia congenitaExpressionCulture modelHuman keratinocytesSiRNASelective inhibition
2006
Stratum-Specific Expression of Human Transferrin Receptor Increases Iron in Mouse Epidermis
Milstone LM, Adams BD, Zhou J, Sanchez V, Shofner J. Stratum-Specific Expression of Human Transferrin Receptor Increases Iron in Mouse Epidermis. Journal Of Investigative Dermatology 2006, 126: 648-652. PMID: 16424878, PMCID: PMC2243218, DOI: 10.1038/sj.jid.5700127.Peer-Reviewed Original ResearchConceptsTransgenic miceStratum-specific expressionTransferrin receptorKeratin 14 promoterTransferrin receptor expressionBody iron lossesReceptor expressionHistological changesSite-dependent differencesLittermate controlsNormal epidermisReceptorsMiceMouse epidermisEpidermal desquamationFerritin expressionHuman transferrin receptorInvolucrin promoterKeratinocytesTransgenic animalsEpidermisAgeExtra ironExpressionAnimals
1977
δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin
Milstone L, Piatigorsky J. δ-Crystallin gene expression in embryonic chick lens epithelia cultured in the presence of insulin. Experimental Cell Research 1977, 105: 9-14. PMID: 837998, DOI: 10.1016/0014-4827(77)90147-1.Peer-Reviewed Original ResearchConceptsEmbryonic lens epitheliumΔ-crystallin synthesisMessenger RNAΔ-crystallinGene expressionLens epitheliumPresence of insulinCultured lens epitheliumFetal calf serumChick lens epitheliumLens epithelial cellsCell elongationTranslational efficiencyCalf serumPolyacrylamide gel electrophoresisTotal protein synthesisProtein synthesisMolecular hybridizationGel electrophoresisSodium dodecyl sulfate-gradient polyacrylamide gel electrophoresisEpithelial cellsSynthesis increasesCellsExpressionHours of culture