Single-cell analysis reveals a subpopulation of adipose progenitor cells that impairs glucose homeostasis
Wang H, Du Y, Huang S, Sun X, Ye Y, Sun H, Chu X, Shan X, Yuan Y, Shen L, Bi Y. Single-cell analysis reveals a subpopulation of adipose progenitor cells that impairs glucose homeostasis. Nature Communications 2024, 15: 4827. PMID: 38844451, PMCID: PMC11156882, DOI: 10.1038/s41467-024-48914-w.Peer-Reviewed Original ResearchConceptsAdipose progenitor cellsT2D patientsProgenitor cellsDiphtheria toxin A expressionHeterogeneous stromal cellsGlycemic disturbancesAdipose tissueInfluence of obesityGlucose homeostasisVisceral adipose tissueHuman visceral adipose tissueImpaired glucose homeostasisType 2 diabetesHunter-killer peptidesRegulating glucose homeostasisSingle-cell analysisAPC functionStromal cellsA expressionMetabolic homeostasisAdipocyte lipolysisT2D developmentPatientsT2DBioactive proteinsSUMO-specific protease 1 regulates germinal center B cell response through deSUMOylation of PAX5
Qi J, Yan L, Sun J, Huang C, Su B, Cheng J, Shen L. SUMO-specific protease 1 regulates germinal center B cell response through deSUMOylation of PAX5. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2314619121. PMID: 38776375, PMCID: PMC11145296, DOI: 10.1073/pnas.2314619121.Peer-Reviewed Original ResearchConceptsPaired box protein 5GC B cellsSUMO-specific protease 1Activation-induced cytidine deaminaseProtein SUMOylationClass switch recombinationProtein stabilityB cellsProtease 1B cell responsesProtein 5Cytidine deaminaseSENP1Up-regulatedGC B cell responsesSomatic hypermutationSUMOylationDeSUMOylationGerminal centersHigher affinityProduction of class-switched antibodiesGerminal center B cell responsesGC reactionMemory B cellsClass-switched antibodiesSingle-cell transcriptomics reveal distinct immune-infiltrating phenotypes and macrophage–tumor interaction axes among different lineages of pituitary neuroendocrine tumors
Lin S, Dai Y, Han C, Han T, Zhao L, Wu R, Liu J, Zhang B, Huang N, Liu Y, Lai S, Shi J, Wang Y, Lou M, Xie J, Cheng Y, Tang H, Yao H, Fang H, Zhang Y, Wu X, Shen L, Ye Y, Xue L, Wu Z. Single-cell transcriptomics reveal distinct immune-infiltrating phenotypes and macrophage–tumor interaction axes among different lineages of pituitary neuroendocrine tumors. Genome Medicine 2024, 16: 60. PMID: 38658971, PMCID: PMC11040908, DOI: 10.1186/s13073-024-01325-4.Peer-Reviewed Original ResearchConceptsTumor immune microenvironmentTumor-associated macrophagesTumor cell apoptosisTumor cellsImmune cellsNeuroendocrine tumorsQuantitative immunofluorescenceComposition of immune cellsInfiltration of immune cellsMultiplexed quantitative immunofluorescenceLevel of immune infiltrationScRNA-seqPituitary neuroendocrine tumorsCX3CR1+ macrophagesDiversity of tumorsCell apoptosisSignificance of macrophagesRNA sequencing samplesScRNA-seq dataCX3CR1+Subcutaneous xenograft experimentsImmune microenvironmentSingle-cell RNA sequencingImmunological environmentImmune infiltration