Layne Dylla
Assistant Professor of Emergency MedicineDownloadHi-Res Photo
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Emergency Medicine
Primary
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Emergency Medicine
Primary
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Are You a Patient?
View this doctor's clinical profile on the Yale Medicine website for information about the services we offer and making an appointment.
View Doctor ProfileAppointments
Emergency Medicine
Primary
Contact Info
Are You a Patient?
View this doctor's clinical profile on the Yale Medicine website for information about the services we offer and making an appointment.
View Doctor ProfileAbout
Titles
Assistant Professor of Emergency Medicine
Biography
Dr. Dylla has a passion for medicine and using research to advance her treatment of many patients. She pursued this passion for medicine and research as dual MD/PhD student at the University of Colorado in the Medical Scientist Training program where she studied the oncogenic role of microRNAs in Ewing Sarcoma. Dr. Dylla subsequently completed her Emergency Medicine Residency training and a dual Experimental Therapeutic and Emergency Medicine Research fellowships at the University of Rochester Medical Center. Since fellowship, Dr. Dylla has been the recipient of several career development and pilot study research grants. These have included an NIH Building Interdisciplinary Research Careers in Women’s Health K12 and an American Heart Association Career Development award. Her work continues to focus on improving patient care and developing new acute stroke treatments in the prehospital and ED settings.
Appointments
Emergency Medicine
Assistant ProfessorPrimary
Other Departments & Organizations
- Emergency Medicine
- Yale Medicine
Education & Training
- MS
- University of Rochester , Clinical Science Investigation (2020)
- Research Fellow
- University of Rochester Medical Center (2020)
- Experimental Therapeutics Fellow
- University of Rochester Medical Center (2019)
- Residency
- University of Rochester Medical Center (2018)
- MD/PhD
- University of Colorado School of Medicine (2015)
Research
Overview
Medical Research Interests
Ischemic Stroke
ORCID
0000-0002-5664-779X
Research at a Glance
Publications Timeline
A big-picture view of Layne Dylla's research output by year.
Research Interests
Research topics Layne Dylla is interested in exploring.
33Publications
206Citations
Ischemic Stroke
Publications
2024
Sex Differences in Tryptophan Metabolism via the Kynurenine Pathway in Acute Ischemic Stroke
Dylla L, Higgins H, Poisson S, Vu T, Reisz J, Herson P, Monte A. Sex Differences in Tryptophan Metabolism via the Kynurenine Pathway in Acute Ischemic Stroke. Clinical Therapeutics 2024 PMID: 39603869, DOI: 10.1016/j.clinthera.2024.10.015.Peer-Reviewed Original ResearchCitationsConceptsAcute ischemic strokeQuinolinic acid levelsLevels of tryptophanNIHSS scoreTryptophan metabolismMetabolite levelsKynurenine pathwayLinear regression modelsIncreased lifetime risk of strokeLifetime risk of strokeMass spectrometry-based metabolomicsPotential sex differencesDownstream kynurenine metabolitesAssociated with poor outcomesIschemic strokeKynurenine pathway metabolitesDecreased tryptophan concentrationsIncreased lifetime riskRegression modelsMultivariate linear regression modelSex differencesExperience worse outcomesRisk of strokeAcid levelsKynurenine metabolitesNanoparticle-Binding Immunoglobulins Predict Variable Complement Responses in Healthy and Diseased Cohorts
Li Y, Saba L, Scheinman R, Banda N, Holers M, Monte A, Dylla L, Moghimi S, Simberg D. Nanoparticle-Binding Immunoglobulins Predict Variable Complement Responses in Healthy and Diseased Cohorts. ACS Nano 2024, 18: 28649-28658. PMID: 39395006, DOI: 10.1021/acsnano.4c05087.Peer-Reviewed Original ResearchAltmetricMeSH Keywords and ConceptsConceptsPegylated liposomal doxorubicinComplement activationPredictive valuePlasma concentrationsC-reactive protein levelsAnti-PEG IgGTriggers proinflammatory responsesAnti-PEG IgMEfficacy of nanomedicinesPoor predictive valueAnti-PEG antibodiesLiposomal doxorubicinIron oxide nanowormsHealthy donorsHigher complement activationAcute infectionInflammatory disease conditionsChronic inflammationProinflammatory responseSystemic administrationComplement cascadeComplement factorsComplement responseIgMProtein levelsSex Differences in the Blood Metabolome During Acute Response to Ischemic Stroke
Dylla L, Higgins H, Stephenson D, Reisz J, Vu T, Poisson S, Herson P, Monte A. Sex Differences in the Blood Metabolome During Acute Response to Ischemic Stroke. Journal Of Women's Health 2024, 33: 1378-1384. PMID: 38946610, PMCID: PMC11564668, DOI: 10.1089/jwh.2023.1133.Peer-Reviewed Original ResearchCitationsAltmetricConceptsIschemic strokeLinear regression modelsMass spectrometry-based metabolomicsLifetime risk of strokeAssociated with neurological outcomeDifferentially expressed metabolitesRegression modelsAcute ischemic strokeRisk of strokeComprehensive stroke centerBlood metabolomeNeurological outcomeAcute treatmentMetabolomic profilesLifetime riskNeurological functionIndividual metabolitesTherapeutic potentialStroke centersAcute responseResults:</i></b>Study objectiveMetabolomicsStrokeNIHSSIdentification of specific recommendations for prehospital stroke care associated with shorter door-to-CT times – An analysis of Get with the Guidelines-Stroke registry and prehospital data
Dylla L, Higgins H, Wham C, Leppert M, Ravare B, Jeppson K, Bina H, Monte A, Poisson S. Identification of specific recommendations for prehospital stroke care associated with shorter door-to-CT times – An analysis of Get with the Guidelines-Stroke registry and prehospital data. Frontiers In Stroke 2024, 3: 1355889. DOI: 10.3389/fstro.2024.1355889.Peer-Reviewed Original ResearchAltmetricConceptsDoor-to-CT timeAmerican Heart AssociationGuidelines-Stroke registryAmerican Heart Association recommendationsPrehospital careStroke patientsEmergency medical services documentationIschemic stroke patientsDoor-to-computed tomographyCare of stroke patientsPrehospital medical recordsPrehospital Stroke ScaleCare of strokeMultivariate linear regression modelPrehospital providersPrehospital dataProvider educationStroke signsPotential confoundersLinear regression modelsTreatment of acute strokeComprehensive stroke centerDescriptive statisticsSuspected strokeMedical recordsOptimized Enzyme-Linked Immunosorbent Assay for Anti-PEG Antibody Detection in Healthy Donors and Patients Treated with PEGylated Liposomal Doxorubicin
Li Y, Ettah U, Jacques S, Gaikwad H, Monte A, Dylla L, Guntupalli S, Moghimi S, Simberg D. Optimized Enzyme-Linked Immunosorbent Assay for Anti-PEG Antibody Detection in Healthy Donors and Patients Treated with PEGylated Liposomal Doxorubicin. Molecular Pharmaceutics 2024, 21: 3053-3060. PMID: 38743264, DOI: 10.1021/acs.molpharmaceut.4c00278.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsEnzyme-linked immunosorbent assayLiposomal doxorubicinHealthy donorsPatients treated with pegylated liposomal doxorubicinEnzyme-linked immunosorbent assay signalCancer patientsAnti-PEG IgGPegylated liposomal doxorubicinImmunosorbent assayOptimized ELISAEnzyme-linked immunosorbent assay protocolsAnti-PEG antibodiesChemotherapy cyclesBetween-assay variabilityPEGylated liposomesCutoff valueAccelerated clearanceTreatment cyclesDrug delivery systemsAntibody titersComplement activationInfused drugAnti-PEGOptimized assayPatientsValidation of dot blot immunoassay for measurement of complement opsonization of nanoparticles
Li Y, Monte A, Dylla L, Moghimi S, Simberg D. Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles. Journal Of Immunological Methods 2024, 528: 113668. PMID: 38574804, PMCID: PMC11023749, DOI: 10.1016/j.jim.2024.113668.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsDeposition of C3Complement activation markersSurface C3 depositionMembrane attack complex C5b-9Activation markersDot blot immunoassayC3 depositionAnaphylatoxins C3aImmune responseNanomedicine researchC5b-9Complement attackComplement activationBlot immunoassayFluid phase markerMarkersPhase markersImmunoassaySurface resultsPathway specificityAssembly of C3 convertaseComplementNanoparticlesAbstract 106: Circulating Interleukin-6 Levels Early During Active Stroke Estimate Time of Stroke Onset: Potential New Clinical Biomarker Tool From a Mobile Stroke Unit
Kowalski R, Ledreux A, Violette J, Neumann R, Grotta J, Ornelas D, Thompson J, Monte A, Dylla L, Coughlan C, Yu X, Graner M, Jones W. Abstract 106: Circulating Interleukin-6 Levels Early During Active Stroke Estimate Time of Stroke Onset: Potential New Clinical Biomarker Tool From a Mobile Stroke Unit. Stroke 2024, 55: a106-a106. DOI: 10.1161/str.55.suppl_1.106.Peer-Reviewed Original ResearchConceptsInterleukin-6 levelsMobile stroke unitInterleukin-6Symptom onsetLesion volumeAcute strokeHealthy controlsMobile stroke unit patientsDevelopment of immunomodulatory therapiesMedian interleukin-6Circulating interleukin-6 levelsMeasurement of interleukin-6Associated with lesion volumeMedian initial NIHSS scoreDWI lesion volumeCirculating interleukin-6Plasma interleukin-6Stroke patientsOnset timeIL-6 responseTime of stroke onsetImmunomodulatory therapyMedian ageThrombolysis windowNIHSS score
2023
Provider Perceptions of Oxygenation Strategies for Critically Ill Trauma Patients With and Without Moderate-to-Severe Traumatic Brain Injury
Dylla L, Douin D, Cwik J, Steinwand A, Rice J, Jackson C, Anderson E, Higgins H, Monte A, Ginde A. Provider Perceptions of Oxygenation Strategies for Critically Ill Trauma Patients With and Without Moderate-to-Severe Traumatic Brain Injury. Military Medicine 2023, 188: 166-175. PMID: 37948260, PMCID: PMC11022338, DOI: 10.1093/milmed/usad076.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsCritically ill trauma patientsCritical care providersTraumatic brain injuryCare providersTrauma patientsSupplemental oxygenNon-TBI patientsCross-sectional surveyUniversity of Colorado HospitalCase-based scenariosProviders' perceptionsProvider practicesCritically ill TBI patientsProviders' viewsOxygen useProvider typeAdministration of supplemental oxygenTargeted educationAssociated with increased mortalityBrain injuryOxygen saturation thresholdsModerate-to-severeSupplemental oxygen useFisher's exact testCritically ill patientsResponse to: Acute ischemic stroke and cardioemboli: Does sex matter?
Higgins H, Chen L, Ravare B, Jeppson K, Bina H, Herson P, Monte A, Poisson S, Dylla L. Response to: Acute ischemic stroke and cardioemboli: Does sex matter? The American Journal Of Emergency Medicine 2023, 74: 170-171. PMID: 37867013, PMCID: PMC10792528, DOI: 10.1016/j.ajem.2023.10.014.Peer-Reviewed Original ResearchMeSH KeywordsSex differences in acute ischemic stroke presentation are a matter of infarct location
Higgins H, Chen L, Ravare B, Jeppson K, Bina H, Herson P, Monte A, Poisson S, Dylla L. Sex differences in acute ischemic stroke presentation are a matter of infarct location. The American Journal Of Emergency Medicine 2023, 74: 95-99. PMID: 37802001, PMCID: PMC10843056, DOI: 10.1016/j.ajem.2023.09.046.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsEmergency medical servicesMultivariate logistic regressionStroke symptomsFrequency of patient presentationLogistic regressionStroke screening toolElectronic health recordsRecognition of strokeAcute ischemic strokeInfarct locationClassic symptomsEmergency department treatmentDistribution of strokeIschemic strokeIschemic stroke presentingHealth recordsRetrospective cohort studyMedical servicesUnilateral weaknessAnterior circulation infarctionCohort studyScreening toolStroke presentationEtiology of strokePosterior circulation infarction
Academic Achievements & Community Involvement
activity Society for Academic Emergency Medicine - Research Committee
Professional OrganizationsCommittee MemberDetailsChair the Research Learning Series objective of the SAEM Research Comittee.2019 - Presentactivity Society for Academic Emergency Medicine - Grants Committee
Professional OrganizationsCommittee MemberDetails2024 - Presentactivity American College of Emergency Physicians - Research Committee
Professional OrganizationsCommittee MemberDetails2017 - Presentactivity Society for Academic Emergency Medicine, Annual Meeting
Peer Review Groups and Grant Study SectionsAbstract ReviewerDetails2019 - Presentactivity American College of Emergency Physicians, National Meeting
Peer Review Groups and Grant Study SectionsAbstract ReviewerDetails2019 - Present
Clinical Care
Overview
Clinical Specialties
Emergency Medicine
Board Certifications
Emergency Medicine
- Certification Organization
- AB of Emergency Medicine
- Original Certification Date
- 2019
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Emergency Medicine
464 Congress Ave
New Haven, CT 06519
United States