2013
NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson’s disease
Korutla L, Furlong H, Mackler S. NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson’s disease. Neuroscience 2013, 257: 86-95. PMID: 24231739, DOI: 10.1016/j.neuroscience.2013.11.001.Peer-Reviewed Original ResearchMeSH KeywordsAged, 80 and overAnimalsBrainCell Line, TransformedCentral Nervous SystemCysteine Proteinase InhibitorsCytoplasmDown-RegulationGlutathione TransferaseHumansImmunoprecipitationLeupeptinsMaleMiceNerve Tissue ProteinsNeuronsParkinson DiseaseProteasome Endopeptidase ComplexRepressor ProteinsUbiquitin-Protein LigasesConceptsNucleus accumbens-1Neuronal cell deathParkinson's diseaseDisease patientsParkinson's disease patientsProteasomal activityPOZ/BTB proteinCell deathNeuronal cellsDiseaseParkin levelsProtein levelsCell susceptibilityParkin proteinProteasome protein levelsPatientsProteasome activityUbiquitin-dependent proteasome degradationCell viabilityParkin degradationDeathParkinKey proteinsProteasome degradationToxicity
2012
Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis
Scofield M, Korutla L, Jackson T, Kalivas P, Mackler S. Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis. Neuroscience 2012, 227: 44-54. PMID: 23022214, PMCID: PMC3505276, DOI: 10.1016/j.neuroscience.2012.09.043.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAspartic AcidCell DeathCholine O-AcetyltransferaseCytoplasmDNA-Binding ProteinsEmbryo, MammalianGene Expression RegulationGlial Fibrillary Acidic ProteinGlutamic AcidImmunoprecipitationNeoplasm ProteinsNeuronsPhosphopyruvate HydrataseProtein BindingRatsRepressor ProteinsSpinal CordTransfectionUbiquitinationConceptsNucleus accumbens-1Amyotrophic lateral sclerosisPOZ/BTB domainUbiquitin-proteasome systemTDP-43Protein 43Lateral sclerosisBTB domainPrimary spinal cord culturesDevelopment of ALSTAR DNA-binding protein 43POZ/BTB proteinDNA-binding protein 43Extracellular glutamate levelsTDP-43 expressionFull-length TDP-43Spinal cord culturesGST pulldown assaysPost-translational modificationsUbiquitinated protein aggregatesCholinergic neuronsGlutamate toxicityCord culturesGlutamate levelsSpinal cord
2007
NAC1 Regulates the Recruitment of the Proteasome Complex into Dendritic Spines
Shen H, Korutla L, Champtiaux N, Toda S, LaLumiere R, Vallone J, Klugmann M, Blendy J, Mackler S, Kalivas P. NAC1 Regulates the Recruitment of the Proteasome Complex into Dendritic Spines. Journal Of Neuroscience 2007, 27: 8903-8913. PMID: 17699672, PMCID: PMC6672176, DOI: 10.1523/jneurosci.1571-07.2007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBicucullineCell Cycle ProteinsCells, CulturedCerebral CortexCullin ProteinsCysteine Proteinase InhibitorsDendritic SpinesEmbryo, MammalianGABA AntagonistsGreen Fluorescent ProteinsIntracellular Signaling Peptides and ProteinsLeupeptinsMiceMice, KnockoutNerve Tissue ProteinsNeuronsProteasome Endopeptidase ComplexProtein TransportRatsRepressor ProteinsRNA-Binding ProteinsTransfectionUbiquitinConceptsUbiquitin-proteasome systemDendritic spinesPostsynaptic densityProteolysis machineryProteasome complexTranscriptional proteinsProteasomeGene deletionSynaptic proteinsProteinCortical neuronsSynaptic remodelingSynaptic activitySynaptic plasticityBicucullinePotential missing linkRecruitmentSpineNeuronsNaC1Progressive accumulationMov34CullinMachineryProteolysis
2005
The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures
Korutla L, Wang P, Mackler S. The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures. Journal Of Neurochemistry 2005, 94: 786-793. PMID: 16033423, DOI: 10.1111/j.1471-4159.2005.03206.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternButyratesCells, CulturedEnzyme InhibitorsGene Expression RegulationHippocampusHistone DeacetylasesHumansHydroxamic AcidsImmunoprecipitationMiceNerve Tissue ProteinsNeuroblastomaNeuronsPlasmidsProtein BindingProtein IsoformsRatsRepressor ProteinsTranscription, GeneticTransfectionTwo-Hybrid System TechniquesConceptsPOZ/BTB proteinProtein-protein interactionsBTB proteinsHistone deacetylasesPOZ domainTwo-hybridGlutathione S-transferase pulldownRecruit histone deacetylasesDifferent histone deacetylasesTranscriptional repressionGST pulldownSNAC1Histone deacetylase inhibitionCorepressorDeacetylasesRepressionDeacetylase inhibitionProteinPulldownHDAC-3NaC1MSin3ANucleus accumbensCNS regionsCoimmunoprecipitation
2003
NAC1, a POZ/BTB protein present in the adult mammalian brain, triggers apoptosis after adenovirus-mediated overexpression in PC-12 cells
Korutla L, Neustadter J, Fournier K, Mackler S. NAC1, a POZ/BTB protein present in the adult mammalian brain, triggers apoptosis after adenovirus-mediated overexpression in PC-12 cells. Neuroscience Research 2003, 46: 33-39. PMID: 12725910, DOI: 10.1016/s0168-0102(03)00024-5.Peer-Reviewed Original ResearchConceptsPC-12 cellsCell deathPOZ/BTB proteinPro-apoptotic protein BaxPOZ/BTBAnti-apoptotic protein Bcl-2Mammalian brainAdenoviral-mediated gene transferProtein Bcl-2BTB proteinsTranscription factorsCellular developmentGenomic DNAAdult mammalian brainNAC1 expressionProtein BaxCell survivalGene transferUpstream mediatorDown regulationNAC1 overexpressionBcl-2Examples actProteinOverexpression