2016
Claudin-3 and claudin-19 partially restore native phenotype to ARPE-19 cells via effects on tight junctions and gene expression
Peng S, Wang SB, Singh D, Zhao PY, Davis K, Chen B, Adelman RA, Rizzolo LJ. Claudin-3 and claudin-19 partially restore native phenotype to ARPE-19 cells via effects on tight junctions and gene expression. Experimental Eye Research 2016, 151: 179-189. PMID: 27593915, DOI: 10.1016/j.exer.2016.08.021.Peer-Reviewed Original ResearchCells, CulturedClaudin-3ClaudinsGene Expression RegulationHumansImmunoblottingMicroscopy, ConfocalPhenotypeRetinal DiseasesRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight Junctions
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportBlood-Retinal BarrierCell LineCells, CulturedClaudin-3Embryonic Stem CellsFetusHumansRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight JunctionsTissue EngineeringTranscriptomeConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina
2011
Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium
Peng S, Rao VS, Adelman RA, Rizzolo LJ. Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2011, 52: 1392-1403. PMID: 21071746, PMCID: PMC3101667, DOI: 10.1167/iovs.10-5984.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsCells, CulturedClaudinsElectric ImpedanceFluorescent Antibody Technique, IndirectGene SilencingGestational AgeHumansImmunoblottingMembrane ProteinsOccludinPermeabilityPolyethylene GlycolsRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingTight JunctionsConceptsRetinal pigment epitheliumTransepithelial electrical resistanceHuman retinal pigment epitheliumHuman fetal RPECultured human fetal retinal pigment epitheliumPigment epitheliumClaudin-19Retinal sideHuman fetal retinal pigment epitheliumSpread of edemaFetal retinal pigment epitheliumFetal human retinal pigment epitheliumEffect of serumTight junctionsRPE permeabilityQuantitative RT-PCRChoroidal capillariesSubpopulation of cellsClaudin-1RPE barrierClaudin-3RPE tight junctionsRT-PCREpitheliumSerum
2009
Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions
Peng S, Adelman RA, Rizzolo LJ. Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2009, 51: 3216-3225. PMID: 20042644, PMCID: PMC2891474, DOI: 10.1167/iovs.09-4162.Peer-Reviewed Original ResearchAngiogenesis InhibitorsAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedBevacizumabBlood-Retinal BarrierCapillary PermeabilityCells, CulturedClaudinsElectric ImpedanceEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoblottingPolyethylene GlycolsPotassiumRanibizumabRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumTight JunctionsVascular Endothelial Growth Factor A
2007
Analysis of the RPE transcriptome reveals dynamic changes during the development of the outer blood-retinal barrier.
Rizzolo LJ, Chen X, Weitzman M, Sun R, Zhang H. Analysis of the RPE transcriptome reveals dynamic changes during the development of the outer blood-retinal barrier. Molecular Vision 2007, 13: 1259-73. PMID: 17679949.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBlood-Retinal BarrierCell Cycle ProteinsChick EmbryoCluster AnalysisCollagenExtracellular MatrixGene Expression ProfilingIntercellular JunctionsLamininMembrane ProteinsMicrotubule-Associated ProteinsMyosinsOligonucleotide Array Sequence AnalysisPhagocytosisPigment Epithelium of EyeProtein SubunitsProteinsReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTranscription, GeneticConceptsPattern of expressionExtensive remodelingAffymetrix microarray chipsCell-cell junctionsSignal transduction pathwaysExtracellular matrix interactionsAdherens junction proteinsTranscellular ion transportCell surface receptorsGenomic approachesEntire genomeGene OntologyTransduction pathwaysSTEM softwareZO-3Biological pathwaysFunctional clusteringMatrix interactionsExtracellular matrixSurface receptorsJunctional complexesMicroarray chipContinuous remodelingFalse discovery rateAffymetrix software
2006
Effects of Culture Conditions on Heterogeneity and the Apical Junctional Complex of the ARPE-19 Cell Line
Luo Y, Zhuo Y, Fukuhara M, Rizzolo LJ. Effects of Culture Conditions on Heterogeneity and the Apical Junctional Complex of the ARPE-19 Cell Line. Investigative Ophthalmology & Visual Science 2006, 47: 3644-3655. PMID: 16877439, DOI: 10.1167/iovs.06-0166.Peer-Reviewed Original ResearchCell Culture TechniquesCell Membrane PermeabilityCytoskeletal ProteinsElectric ConductivityElectric ImpedanceElectrophysiologyHorseradish PeroxidaseHumansImmunoblottingImmunohistochemistryMembrane ProteinsMicroscopy, FluorescenceOccludinPigment Epithelium of EyeReverse Transcriptase Polymerase Chain ReactionTight JunctionsExpression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions
Luo Y, Fukuhara M, Weitzman M, Rizzolo LJ. Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions. Brain Research 2006, 1110: 55-63. PMID: 16859655, DOI: 10.1016/j.brainres.2006.06.059.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsBlotting, WesternCell Adhesion MoleculesCells, CulturedChick EmbryoCulture Media, ConditionedGene Expression Regulation, DevelopmentalMyosinsPigment Epithelium of EyeRetinaReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight Junctions
2004
The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development
Rahner C, Fukuhara M, Peng S, Kojima S, Rizzolo LJ. The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development. Journal Of Cell Science 2004, 117: 3307-3318. PMID: 15226402, DOI: 10.1242/jcs.01181.Peer-Reviewed Original ResearchAnimalsCell SeparationCells, CulturedChick EmbryoCulture Media, ConditionedDNA PrimersElectric ImpedanceElectrophysiologyFibroblastsFreeze FracturingGene Expression Regulation, DevelopmentalMicroscopy, FluorescenceNerve Tissue ProteinsPigment Epithelium of EyePolymerase Chain ReactionRetinaReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight JunctionsTime Factors
2001
Defects in the MITFmi / miApical Surface are Associated with a Failure of Outer Segment Elongation
Bumsted K, Rizzolo L, Barnstable C. Defects in the MITFmi / miApical Surface are Associated with a Failure of Outer Segment Elongation. Experimental Eye Research 2001, 73: 383-392. PMID: 11520113, DOI: 10.1006/exer.2001.1048.Peer-Reviewed Original Research
2000
Regulation of glucose transporters during development of the retinal pigment epithelium
Ban Y, Rizzolo L. Regulation of glucose transporters during development of the retinal pigment epithelium. Brain Research 2000, 121: 89-95. PMID: 10837896, DOI: 10.1016/s0165-3806(00)00028-6.Peer-Reviewed Original ResearchAnimalsBlood-Brain BarrierBlotting, NorthernChick EmbryoGene Expression Regulation, DevelopmentalGlucose Transporter Type 1Glucose Transporter Type 2Glucose Transporter Type 3Monosaccharide Transport ProteinsNerve Tissue ProteinsPigment Epithelium of EyeReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTight Junctions