2016
Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices
Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomaterialia 2016, 46: 91-100. PMID: 27693690, PMCID: PMC5451113, DOI: 10.1016/j.actbio.2016.09.043.Peer-Reviewed Original ResearchConceptsExtracellular matrixLung extracellular matrixMatrix proteinsECM-associated proteinsCell-matrix interactionsProtein extraction methodsWhole organ regenerationRegenerative medicineOrganotypic cell culturesQuantitative proteomicsAcellular extracellular matrixECM proteinsOrgan regenerationCell adhesionProteomicsProtein analysisQuantitative biochemical dataProteinPotent substrateXenogeneic extracellular matrixTargeted proteomicsCell nuclear antigenBiochemical dataImportant glycoproteinCell culturesEngineered Tissue–Stent Biocomposites as Tracheal Replacements
Zhao L, Sundaram S, Le AV, Huang AH, Zhang J, Hatachi G, Beloiartsev A, Caty MG, Yi T, Leiby K, Gard A, Kural MH, Gui L, Rocco KA, Sivarapatna A, Calle E, Greaney A, Urbani L, Maghsoudlou P, Burns A, DeCoppi P, Niklason LE. Engineered Tissue–Stent Biocomposites as Tracheal Replacements. Tissue Engineering Part A 2016, 22: 1086-1097. PMID: 27520928, PMCID: PMC5312617, DOI: 10.1089/ten.tea.2016.0132.Peer-Reviewed Original ResearchComparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effectImplantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraft
2013
Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix
Ghaedi M, Calle EA, Mendez JJ, Gard AL, Balestrini J, Booth A, Bove PF, Gui L, White ES, Niklason LE. Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix. Journal Of Clinical Investigation 2013, 123: 4950-4962. PMID: 24135142, PMCID: PMC3809786, DOI: 10.1172/jci68793.Peer-Reviewed Original ResearchMeSH KeywordsAlveolar Epithelial CellsAnimalsBiomarkersCell AdhesionCell DifferentiationCell ProliferationExtracellular MatrixHumansInduced Pluripotent Stem CellsMiceMucin-1Pulmonary AlveoliPulmonary Surfactant-Associated Protein BPulmonary Surfactant-Associated Protein CRatsTissue EngineeringTissue ScaffoldsConceptsAlveolar epithelial type IILung tissueInduced pluripotent stem cellsHuman induced pluripotent stem cellsWnt/β-catenin inhibitorAcellular lung matrixHuman adult lungRespiratory epithelial cellsΒ-catenin inhibitorType I cellsLung tissue engineeringLung extracellular matrixLung matrixAlveolar epitheliumPulmonary epitheliumAlveolar cellsAdult lungCell therapySurfactant protein CMucin 1Protein CEpithelial cellsProgenitor cellsI cellsSurfactant protein B
2011
Readily Available Tissue-Engineered Vascular Grafts
Dahl SL, Kypson AP, Lawson JH, Blum JL, Strader JT, Li Y, Manson RJ, Tente WE, DiBernardo L, Hensley MT, Carter R, Williams TP, Prichard HL, Dey MS, Begelman KG, Niklason LE. Readily Available Tissue-Engineered Vascular Grafts. Science Translational Medicine 2011, 3: 68ra9. PMID: 21289273, DOI: 10.1126/scitranslmed.3001426.Peer-Reviewed Original ResearchConceptsTissue-engineered vascular graftsVascular graftsMechanical propertiesSynthetic graftsAutologous tissueCoronary artery bypassCanine smooth muscle cellsPolyglycolic acid scaffoldsTissue engineeringHuman vascular graftsSmooth muscle cellsSynthetic vascular graftsArtery bypassSuch patientsArteriovenous accessArterial bypassHuman blood vesselsCardiovascular diseaseExcellent patencyBaboon modelIntimal hyperplasiaDog modelCellular materialPatientsGraft
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchange