2016
New Functional Tools for Antithrombogenic Activity Assessment of Live Surface Glycocalyx
Dimitrievska S, Gui L, Weyers A, Lin T, Cai C, Wu W, Tuggle CT, Sundaram S, Balestrini JL, Slattery D, Tchouta L, Kyriakides TR, Tarbell JM, Linhardt RJ, Niklason LE. New Functional Tools for Antithrombogenic Activity Assessment of Live Surface Glycocalyx. Arteriosclerosis Thrombosis And Vascular Biology 2016, 36: 1847-1853. PMID: 27386939, PMCID: PMC5283952, DOI: 10.1161/atvbaha.116.308023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntithrombinsAorta, ThoracicBiological AssayBlood CoagulationCells, CulturedChromatography, LiquidFactor XaGlycocalyxHeparinHeparitin SulfateHuman Umbilical Vein Endothelial CellsMaleMass SpectrometryMicroscopy, Electron, TransmissionRats, Sprague-DawleyReproducibility of ResultsThrombinTime FactorsConceptsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVein endothelial cellsNative aortaEndothelial cellsHuman umbilical vein endothelial cell monolayersCultured human umbilical vein endothelial cellsFactor XaVascular healthEndothelial cell monolayersVascular diseaseRat aortaDamaged vasculatureAnticoagulant capacityVascular communityAortaTherapeutic developmentActivity assessmentCell monolayersVascular graftsLiquid chromatography-mass spectrometry analysisNovel assayGlycocalyxAssaysNative rat aortaImplantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraftBioengineered human acellular vessels for dialysis access in patients with end-stage renal disease: two phase 2 single-arm trials
Lawson JH, Glickman MH, Ilzecki M, Jakimowicz T, Jaroszynski A, Peden EK, Pilgrim AJ, Prichard HL, Guziewicz M, Przywara S, Szmidt J, Turek J, Witkiewicz W, Zapotoczny N, Zubilewicz T, Niklason LE. Bioengineered human acellular vessels for dialysis access in patients with end-stage renal disease: two phase 2 single-arm trials. The Lancet 2016, 387: 2026-2034. PMID: 27203778, PMCID: PMC4915925, DOI: 10.1016/s0140-6736(16)00557-2.Peer-Reviewed Original ResearchConceptsHuman acellular vesselEnd-stage renal diseaseAcellular vesselsPrimary patencySecondary patencyRenal diseaseDialysis accessHaemodialysis accessPhase 2 single-arm trialSingle-arm phase 2 trialPolytetrafluoroethylene arteriovenous graftsPhase 2 trialDialysis access graftsSingle-arm trialArms of patientsPrimary endpointChronic haemodialysisAccess graftsUS National InstitutesArteriovenous graftsVenous anastomosisCensoring eventIntimal hyperplasiaPatencyPatients
2012
Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells
Zhou J, Niklason LE. Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells. Integrative Biology 2012, 4: 1487-1497. PMID: 23114826, PMCID: PMC3628532, DOI: 10.1039/c2ib00171c.Peer-Reviewed Original ResearchMeSH KeywordsBeta CateninBiomechanical PhenomenaBlood VesselsCell DifferentiationCells, CulturedEquipment DesignHumansMesenchymal Stem CellsMicrofluidic Analytical TechniquesSignal TransductionSmad ProteinsStress, MechanicalSystems BiologyTissue EngineeringTransforming Growth Factor betaWnt Signaling PathwayConceptsVascular tissue engineeringMicrofluidic channelTissue engineeringDynamic mechanical stimulationHydrodynamic actuationDeformable membraneDevice fatigueStem cell mechanobiologyCyclic strainComplex mechanical stimulationMechanical environmentCell mechanobiologyMesenchymal stem cellsPerformance degradationCyclic stimulationStem cellsBiomechanical conditionsCyclic circumferential strainMicrochip platformSignal transduction cascadeMechanical stimulationVascular cell lineagesVersatile platformEngineeringCanonical Wnt/β-catenin
2011
Readily Available Tissue-Engineered Vascular Grafts
Dahl SL, Kypson AP, Lawson JH, Blum JL, Strader JT, Li Y, Manson RJ, Tente WE, DiBernardo L, Hensley MT, Carter R, Williams TP, Prichard HL, Dey MS, Begelman KG, Niklason LE. Readily Available Tissue-Engineered Vascular Grafts. Science Translational Medicine 2011, 3: 68ra9. PMID: 21289273, DOI: 10.1126/scitranslmed.3001426.Peer-Reviewed Original ResearchConceptsTissue-engineered vascular graftsVascular graftsMechanical propertiesSynthetic graftsAutologous tissueCoronary artery bypassCanine smooth muscle cellsPolyglycolic acid scaffoldsTissue engineeringHuman vascular graftsSmooth muscle cellsSynthetic vascular graftsArtery bypassSuch patientsArteriovenous accessArterial bypassHuman blood vesselsCardiovascular diseaseExcellent patencyBaboon modelIntimal hyperplasiaDog modelCellular materialPatientsGraft