2024
Engineered vascular grafts lend unique insight to pathophysiology of aortic aneurysms
Naegeli K, Niklason L. Engineered vascular grafts lend unique insight to pathophysiology of aortic aneurysms. Cell Stem Cell 2024, 31: 1099-1100. PMID: 39094540, DOI: 10.1016/j.stem.2024.07.002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortic AneurysmBlood Vessel ProsthesisHumansInduced Pluripotent Stem CellsLoeys-Dietz SyndromeMyocytes, Smooth MuscleTissue Engineering
2016
Engineered Tissue–Stent Biocomposites as Tracheal Replacements
Zhao L, Sundaram S, Le AV, Huang AH, Zhang J, Hatachi G, Beloiartsev A, Caty MG, Yi T, Leiby K, Gard A, Kural MH, Gui L, Rocco KA, Sivarapatna A, Calle E, Greaney A, Urbani L, Maghsoudlou P, Burns A, DeCoppi P, Niklason LE. Engineered Tissue–Stent Biocomposites as Tracheal Replacements. Tissue Engineering Part A 2016, 22: 1086-1097. PMID: 27520928, PMCID: PMC5312617, DOI: 10.1089/ten.tea.2016.0132.Peer-Reviewed Original ResearchComparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effectImplantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraftBiaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries
Huang AH, Balestrini JL, Udelsman BV, Zhou KC, Zhao L, Ferruzzi J, Starcher BC, Levene MJ, Humphrey JD, Niklason LE. Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries. Tissue Engineering Part C Methods 2016, 22: 524-533. PMID: 27108525, PMCID: PMC4921901, DOI: 10.1089/ten.tec.2015.0309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriesBioreactorsCollagenElastic TissueExtracellular MatrixHumansRegenerationStress, MechanicalTissue EngineeringConceptsTissue-engineered blood vesselsBiaxial loadingMechanical propertiesMechanical strengthFiber orientationMultiaxial loadingLoading conditionsMechanical integrityBiaxial stretchingCollagen undulationArtificial skinNovel bioreactorMechanical failureMatrix orientationBiaxial stretchLoadingAxial stretchCollagen fiber orientationSuture strengthNative arteriesTissue equivalentsStrengthPropertiesCircumferential stretchMatrix content
2014
A Call to Craft
Raredon MS, Niklason LE. A Call to Craft. Science Translational Medicine 2014, 6: 218fs1. PMID: 24401937, PMCID: PMC4122270, DOI: 10.1126/scitranslmed.3008229.Commentaries, Editorials and LettersConceptsTissue engineers
2013
Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix
Ghaedi M, Calle EA, Mendez JJ, Gard AL, Balestrini J, Booth A, Bove PF, Gui L, White ES, Niklason LE. Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix. Journal Of Clinical Investigation 2013, 123: 4950-4962. PMID: 24135142, PMCID: PMC3809786, DOI: 10.1172/jci68793.Peer-Reviewed Original ResearchMeSH KeywordsAlveolar Epithelial CellsAnimalsBiomarkersCell AdhesionCell DifferentiationCell ProliferationExtracellular MatrixHumansInduced Pluripotent Stem CellsMiceMucin-1Pulmonary AlveoliPulmonary Surfactant-Associated Protein BPulmonary Surfactant-Associated Protein CRatsTissue EngineeringTissue ScaffoldsConceptsAlveolar epithelial type IILung tissueInduced pluripotent stem cellsHuman induced pluripotent stem cellsWnt/β-catenin inhibitorAcellular lung matrixHuman adult lungRespiratory epithelial cellsΒ-catenin inhibitorType I cellsLung tissue engineeringLung extracellular matrixLung matrixAlveolar epitheliumPulmonary epitheliumAlveolar cellsAdult lungCell therapySurfactant protein CMucin 1Protein CEpithelial cellsProgenitor cellsI cellsSurfactant protein B
2012
Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells
Zhou J, Niklason LE. Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells. Integrative Biology 2012, 4: 1487-1497. PMID: 23114826, PMCID: PMC3628532, DOI: 10.1039/c2ib00171c.Peer-Reviewed Original ResearchMeSH KeywordsBeta CateninBiomechanical PhenomenaBlood VesselsCell DifferentiationCells, CulturedEquipment DesignHumansMesenchymal Stem CellsMicrofluidic Analytical TechniquesSignal TransductionSmad ProteinsStress, MechanicalSystems BiologyTissue EngineeringTransforming Growth Factor betaWnt Signaling PathwayConceptsVascular tissue engineeringMicrofluidic channelTissue engineeringDynamic mechanical stimulationHydrodynamic actuationDeformable membraneDevice fatigueStem cell mechanobiologyCyclic strainComplex mechanical stimulationMechanical environmentCell mechanobiologyMesenchymal stem cellsPerformance degradationCyclic stimulationStem cellsBiomechanical conditionsCyclic circumferential strainMicrochip platformSignal transduction cascadeMechanical stimulationVascular cell lineagesVersatile platformEngineeringCanonical Wnt/β-catenin
2011
Decellularized tissue-engineered blood vessel as an arterial conduit
Quint C, Kondo Y, Manson RJ, Lawson JH, Dardik A, Niklason LE. Decellularized tissue-engineered blood vessel as an arterial conduit. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 9214-9219. PMID: 21571635, PMCID: PMC3107282, DOI: 10.1073/pnas.1019506108.Peer-Reviewed Original ResearchConceptsTissue-engineered vesselsBiomimetic perfusion systemArterial tissue engineeringTissue-engineered blood vesselsTissue engineering techniquesEndothelial progenitor cellsTissue engineeringRobust extracellular matrixVein graftsBiological vascular graftPorcine carotid arteriesVascular graftsTissue regenerationIntimal hyperplasiaAutologous endothelial progenitor cellsGraft wallProgenitor cellsControl vein graftsSmooth muscle cellsGraft lumenGraft occlusionArterial conduitsImproved patencyCarotid arteryPorcine smooth muscle cellsReadily Available Tissue-Engineered Vascular Grafts
Dahl SL, Kypson AP, Lawson JH, Blum JL, Strader JT, Li Y, Manson RJ, Tente WE, DiBernardo L, Hensley MT, Carter R, Williams TP, Prichard HL, Dey MS, Begelman KG, Niklason LE. Readily Available Tissue-Engineered Vascular Grafts. Science Translational Medicine 2011, 3: 68ra9. PMID: 21289273, DOI: 10.1126/scitranslmed.3001426.Peer-Reviewed Original ResearchConceptsTissue-engineered vascular graftsVascular graftsMechanical propertiesSynthetic graftsAutologous tissueCoronary artery bypassCanine smooth muscle cellsPolyglycolic acid scaffoldsTissue engineeringHuman vascular graftsSmooth muscle cellsSynthetic vascular graftsArtery bypassSuch patientsArteriovenous accessArterial bypassHuman blood vesselsCardiovascular diseaseExcellent patencyBaboon modelIntimal hyperplasiaDog modelCellular materialPatientsGraft
2010
Tissue-Engineered Lungs for in Vivo Implantation
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.Peer-Reviewed Original ResearchConceptsLung tissueLung matrixAcellular lung matrixNative lung tissueTissue-engineered lungsLung transplantationPrimary therapyAdult lung tissueAdult ratsPulmonary epitheliumVascular endotheliumFunctional lungLung regenerationVascular compartmentLungSeeded endothelial cellsMechanical characteristicsEndothelial cellsVivo implantationRatsEpitheliumTissueCellular componentsExtracellular matrixGas exchange
2003
Decellularized native and engineered arterial scaffolds for transplantation.
Dahl S, Koh J, Prabhakar V, Niklason L. Decellularized native and engineered arterial scaffolds for transplantation. Cell Transplantation 2003, 12: 659-66. PMID: 14579934.Peer-Reviewed Original Research