2014
Small transmembrane protein inhibitors of the platelet‐derived growth factor β receptor (LB215)
Petti L, Talbert‐Slagle K, Chacon K, Hochstrasser M, DiMaio D. Small transmembrane protein inhibitors of the platelet‐derived growth factor β receptor (LB215). The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.lb215.Peer-Reviewed Original ResearchTransmembrane domainTransmembrane proteinPlatelet-derived growth factor β receptorProtein inhibitorGrowth factor receptor signalingSingle conservative amino acid substitutionSmall transmembrane proteinConservative amino acid substitutionsGrowth factor β receptorParticular tyrosine residueReceptor tyrosine kinasesAmino acid substitutionsSequence similarityGrowth factor receptorTraptamersReceptor dimerizationEffects of PDGFSmall proteinsTyrosine residuesExtracellular domainTyrosine kinaseAcid substitutionsReceptor signalingRetroviral libraryPDGFβR
2013
A Single Amino Acid Substitution Converts a Transmembrane Protein Activator of the Platelet-derived Growth Factor β Receptor into an Inhibitor*
Petti LM, Talbert-Slagle K, Hochstrasser ML, DiMaio D. A Single Amino Acid Substitution Converts a Transmembrane Protein Activator of the Platelet-derived Growth Factor β Receptor into an Inhibitor*. Journal Of Biological Chemistry 2013, 288: 27273-27286. PMID: 23908351, PMCID: PMC3779723, DOI: 10.1074/jbc.m113.470054.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsBovine papillomavirus 1CattleCell LineCell Transformation, ViralEnzyme ActivatorsFibroblastsHumansMaleMiceMutation, MissenseOncogene Proteins v-sisOncogene Proteins, ViralPhosphorylationProtein Kinase InhibitorsReceptor, Platelet-Derived Growth Factor betaSignal TransductionConceptsTransmembrane domainTransmembrane proteinPlatelet-derived growth factor β receptorLimited sequence similarityGrowth factor β receptorParticular tyrosine residueLigand-independent mannerSequence similarityGenetic evidenceGrowth factor receptorE5 proteinOncogenic transformationWild typeProtein activatorViral homologueTyrosine residuesE5 bindsProtein inhibitorPDGFβRV-sisPDGFβ receptorBovine papillomavirusFactor receptorNonidentical sitesCell proliferation
2009
Artificial Transmembrane Oncoproteins Smaller than the Bovine Papillomavirus E5 Protein Redefine Sequence Requirements for Activation of the Platelet-Derived Growth Factor β Receptor
Talbert-Slagle K, Marlatt S, Barrera FN, Khurana E, Oates J, Gerstein M, Engelman DM, Dixon AM, DiMaio D. Artificial Transmembrane Oncoproteins Smaller than the Bovine Papillomavirus E5 Protein Redefine Sequence Requirements for Activation of the Platelet-Derived Growth Factor β Receptor. Journal Of Virology 2009, 83: 9773-9785. PMID: 19605488, PMCID: PMC2748040, DOI: 10.1128/jvi.00946-09.Peer-Reviewed Original ResearchConceptsPDGF beta receptorTransmembrane domainBPV E5Transmembrane proteinBovine papillomavirus E5 proteinPlatelet-derived growth factor beta receptorArtificial transmembrane proteinsLigand-independent receptor activationPrimary amino acid sequenceSimilar cellular proteinsSmall artificial proteinsGrowth factor β receptorHomodimeric transmembrane proteinAmino acid sequenceGrowth factor beta receptorConsecutive amino acidsC-terminal cysteineSame cellular targetsNew genetic techniquesBeta receptorsCellular proteinsAcid sequenceE5 proteinGenetic techniquesSequence requirements
2008
The bovine papillomavirus E5 protein and the PDGF β receptor: It takes two to tango
Talbert-Slagle K, DiMaio D. The bovine papillomavirus E5 protein and the PDGF β receptor: It takes two to tango. Virology 2008, 384: 345-351. PMID: 18990418, PMCID: PMC2661243, DOI: 10.1016/j.virol.2008.09.033.Peer-Reviewed Original ResearchConceptsBovine papillomavirus E5 proteinE5 proteinTransmembrane domainTransmembrane proteinTarget proteinsPlatelet-derived growth factor beta receptorArtificial transmembrane proteinsMembrane-spanning segmentsHuman erythropoietin receptorBPV E5 proteinGrowth factor beta receptorCellular receptor tyrosine kinasesLigand-independent activationReceptor tyrosine kinasesGenetic screenPDGF β-receptorNovel proteinTransmembrane sequenceCellular proteinsMitogenic signalingHydrophobic proteinsReceptor dimerizationLarger target proteinsErythropoietin receptorSmall proteins