2021
AP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis
White J, Thompson D, Koch K, Kiriazov B, Beck A, van der Heide D, Grimm B, Kulak M, Weigel R. AP-2alpha-mediated activation of E2F and EZH2 drives melanoma metastasis. Cancer Research 2021, 81: canres.0772.2021. PMID: 34210752, PMCID: PMC8416798, DOI: 10.1158/0008-5472.can-21-0772.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex 2Adaptor Protein Complex alpha SubunitsAnimalsBase SequenceBenzamidesBiomarkersBiphenyl CompoundsCell Line, TumorE2F Transcription FactorsEnhancer of Zeste Homolog 2 ProteinEpigenesis, GeneticHumansMelanocytesMelanomaMiceMice, Inbred NODMice, SCIDMorpholinesNeoplasm MetastasisNeoplasm TransplantationNeoplasms, Second PrimaryPromoter Regions, GeneticPyridonesSingle-Cell AnalysisTranscription Factor AP-2ConceptsAP-2αRepression complexEZH2 inhibitorsAP-2α transcription factorSingle-cell RNA sequencing analysisE2F target genesRNA sequencing analysisSpecific EZH2 inhibitorsAP-2α expressionActivation of E2FAnchorage-independent colony formationMelanocyte stem cellsTumor suppressor activityChromatin marksNucleosome remodelingBioID screenE2F pathwayTranscriptional activationTranscription factorsTarget genesPathway genesAP-2alphaSequencing analysisMelanoma metastasesGenes
2020
AP-2γ Is Required for Maintenance of Multipotent Mammary Stem Cells
Gu VW, Cho E, Thompson DT, Cassady VC, Borcherding N, Koch KE, Wu VT, Lorenzen AW, van der Heide DM, White JR, Kulak MV, Williams T, Zhang W, Weigel RJ. AP-2γ Is Required for Maintenance of Multipotent Mammary Stem Cells. Stem Cell Reports 2020, 16: 106-119. PMID: 33382976, PMCID: PMC7897584, DOI: 10.1016/j.stemcr.2020.12.002.Peer-Reviewed Original ResearchConceptsMammary stem cellsAP-2γMultipotent mammary stem cellsMammary gland ductal morphogenesisAP-2γ transcription factorMammary gland structureMammary gland developmentStem cellsMammary epithelial cellsAdult MaSCsBasal epitheliumDuctal outgrowthMammary developmentLuminal progenitorsConditional knockoutEpithelial cellsLuminal lineageMammary lineageDuctal morphogenesisGland developmentNumber of cellsRegenerative capacityGland structureExpression of genesCells