1997
Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes
Clifford E, Martin K, Dalal P, Thomas R, Dubyak G. Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes. American Journal Of Physiology 1997, 273: c973-c987. PMID: 9316419, DOI: 10.1152/ajpcell.1997.273.3.c973.Peer-Reviewed Original ResearchMeSH Keywords5'-NucleotidaseAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphatasesAdenosine TriphosphateAntigens, CDApyraseBucladesineCell DifferentiationCytosolDNA PrimersGene Expression Regulation, NeoplasticHL-60 CellsHumansLeukemiaLeukocytesModels, BiologicalPolymerase Chain ReactionReceptors, Purinergic P2RNA, MessengerTetradecanoylphorbol AcetateTime FactorsTranscription, GeneticTumor Cells, CulturedUridine TriphosphateConceptsKG-1 cellsMyeloid progenitorsStage-specific expressionHL-60 promyelocytesExtracellular ATPMyeloid cell differentiationHL-60P2-purinergic receptor subtypesSignal transductionMyeloid leukocytesHL-60 cellsTranscription-polymerase chain reaction analysisATP receptor subtypesExogenous ATPaseCell differentiationRecent identificationDevelopmental stagesApyrase activityTHP-1 monocytesChain reaction analysisPotato apyraseP2Y receptor subtypesPurinergic receptor subtypesDifferentiationAutocrine mechanism
1996
Recruitment of purinergically stimulated Cl- channels from granule membrane to plasma membrane
Merlin D, Guo X, Martin K, Laboisse C, Landis D, Dubyak G, Hopfer U. Recruitment of purinergically stimulated Cl- channels from granule membrane to plasma membrane. American Journal Of Physiology 1996, 271: c612-c619. PMID: 8770002, DOI: 10.1152/ajpcell.1996.271.2.c612.Peer-Reviewed Original ResearchConceptsHT29-ClGranule fusionCl- channelsFungal metabolite wortmanninCAMP-activated Cl- channelMucin granulesCell linesRegulated exocytosisWortmannin concentrationsPlasma membraneActin depolymerizationWortmannin inhibitionCell line HT-29Granule membranesExtracellular ATPEpithelial cellsFusion inhibitorsHuman adenocarcinoma cell line HT-29WortmanninSecretion releaseCl- conductancePhalloidinMembraneDifferential inhibitionHT-29