2017
Opposing Actions of AKT (Protein Kinase B) Isoforms in Vascular Smooth Muscle Injury and Therapeutic Response
Jin Y, Xie Y, Ostriker AC, Zhang X, Liu R, Lee MY, Leslie KL, Tang W, Du J, Lee SH, Wang Y, Sessa WC, Hwa J, Yu J, Martin KA. Opposing Actions of AKT (Protein Kinase B) Isoforms in Vascular Smooth Muscle Injury and Therapeutic Response. Arteriosclerosis Thrombosis And Vascular Biology 2017, 37: 2311-2321. PMID: 29025710, PMCID: PMC5699966, DOI: 10.1161/atvbaha.117.310053.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCell Cycle ProteinsCell DifferentiationCell MovementCell ProliferationCells, CulturedDisease Models, AnimalForkhead Transcription FactorsGene Expression RegulationGenetic Predisposition to DiseaseHumansMice, KnockoutMuscle, Smooth, VascularMyocytes, Smooth MuscleNeointimaNuclear ProteinsPhenotypePromoter Regions, GeneticProto-Oncogene Proteins c-aktRNA InterferenceRNA, MessengerSignal TransductionSirolimusTime FactorsTrans-ActivatorsTranscription FactorsTransfectionVascular System InjuriesConceptsIntimal hyperplasiaTherapeutic inhibitionVascular smooth muscle injurySmooth muscle-specific deletionSmooth muscle cell proliferationSystemic vascular diseaseSevere intimal hyperplasiaSmooth muscle injuryNew treatment strategiesWild-type miceAkt isoformsMuscle cell proliferationMuscle-specific deletionMechanism of actionVascular smooth muscle cell differentiationCoronary revascularizationSmooth muscle cell differentiationDiabetes mellitusDiabetic patientsControl miceRapamycin therapyVascular diseaseMuscle injuryTherapeutic responseSevere thrombosis
1997
Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes
Clifford E, Martin K, Dalal P, Thomas R, Dubyak G. Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes. American Journal Of Physiology 1997, 273: c973-c987. PMID: 9316419, DOI: 10.1152/ajpcell.1997.273.3.c973.Peer-Reviewed Original ResearchMeSH Keywords5'-NucleotidaseAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphatasesAdenosine TriphosphateAntigens, CDApyraseBucladesineCell DifferentiationCytosolDNA PrimersGene Expression Regulation, NeoplasticHL-60 CellsHumansLeukemiaLeukocytesModels, BiologicalPolymerase Chain ReactionReceptors, Purinergic P2RNA, MessengerTetradecanoylphorbol AcetateTime FactorsTranscription, GeneticTumor Cells, CulturedUridine TriphosphateConceptsKG-1 cellsMyeloid progenitorsStage-specific expressionHL-60 promyelocytesExtracellular ATPMyeloid cell differentiationHL-60P2-purinergic receptor subtypesSignal transductionMyeloid leukocytesHL-60 cellsTranscription-polymerase chain reaction analysisATP receptor subtypesExogenous ATPaseCell differentiationRecent identificationDevelopmental stagesApyrase activityTHP-1 monocytesChain reaction analysisPotato apyraseP2Y receptor subtypesPurinergic receptor subtypesDifferentiationAutocrine mechanismAngiotensin II activates the beta 1 isoform of phospholipase C in vascular smooth muscle cells
Schelling J, Nkemere N, Konieczkowski M, Martin K, Dubyak G. Angiotensin II activates the beta 1 isoform of phospholipase C in vascular smooth muscle cells. American Journal Of Physiology 1997, 272: c1558-c1566. PMID: 9176147, DOI: 10.1152/ajpcell.1997.272.5.c1558.Peer-Reviewed Original ResearchConceptsAortic vascular smooth muscle cellsVascular smooth muscle cellsHuman aortic vascular smooth muscle cellsPLC-beta 1Ang IISmooth muscle cellsPLC beta isoformsPLC-beta 1 antibodyRat aortic vascular smooth muscle cellsPermeabilized vascular smooth muscle cellsMuscle cellsGrowth factorPathophysiology of hypertensionAngiotensin II receptorsIP formationReverse transcriptase-polymerase chain reactionPLC-gamma 1 antibodyTranscriptase-polymerase chain reactionPlatelet-derived growth factorCytosolic free Ca2Gamma 1 antibodiesAng II signalsAngiotensin IIAlpha antibodyII receptorsDown-Regulation of P2U-Purinergic Nucleotide Receptor Messenger RNA Expression During In Vitro Differentiation of Human Myeloid Leukocytes by Phorbol Esters or Inflammatory Activators
Martin K, Kertesy S, Dubyak G. Down-Regulation of P2U-Purinergic Nucleotide Receptor Messenger RNA Expression During In Vitro Differentiation of Human Myeloid Leukocytes by Phorbol Esters or Inflammatory Activators. Molecular Pharmacology 1997, 51: 97-108. PMID: 9016351, DOI: 10.1124/mol.51.1.97.Peer-Reviewed Original ResearchConceptsTHP-1 monocytesHL-60 cellsMRNA levelsMyeloid leukocytesReceptor messenger RNA expressionMonocyte/macrophage phenotypeMessenger RNA expressionInositol phospholipid hydrolysisNeutrophil phenotypeInflammatory activationHL-60 granulocytesUndifferentiated HL-60 cellsInflammatory activatorsInflammatory macrophagesAcute exposureMacrophage phenotypeNucleotide receptorsHuman epithelial cellsTissue-specific mechanismsEpithelial cellsRNA expressionLeukocytesDown regulationDibutyryl cAMPHuman keratinocytes