2005
Multiple melanocortin receptors are expressed in bone cells
Zhong Q, Sridhar S, Ruan L, Ding KH, Xie D, Insogna K, Kang B, Xu J, Bollag RJ, Isales CM. Multiple melanocortin receptors are expressed in bone cells. Bone 2005, 36: 820-831. PMID: 15804492, DOI: 10.1016/j.bone.2005.01.020.Peer-Reviewed Original ResearchMeSH KeywordsAdrenocorticotropic HormoneAnimalsBase SequenceBlotting, NorthernBlotting, SouthernCyclic AMPDNA PrimersHumansImmunohistochemistryMiceOligonucleotide Array Sequence AnalysisOsteoblastsPro-OpiomelanocortinProtein BindingRatsReceptors, CorticotropinReceptors, MelanocortinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerThymidineConceptsMelanocortin receptorsBone turnoverOsteoclastic cellsReceptor familySteroid hormone secretionBone cellsMelanocortin receptor familyFamily of receptorsHormone secretionBeta-endorphinSpecific effectsPOMC fragmentsPOMC mRNACell subpopulationsAlpha betaGamma-MSHReceptorsPeptide hormonesPOMCG proteinsVivo dataBoneHormoneOsteoblastic cellsVariety of genes
1996
Tumor necrosis factor‐α induces transcription of the colony‐stimulating factor‐1 gene in murine osteoblasts
Kaplan D, Eielson C, Horowitz M, Insogna K, Weir E. Tumor necrosis factor‐α induces transcription of the colony‐stimulating factor‐1 gene in murine osteoblasts. Journal Of Cellular Physiology 1996, 168: 199-208. PMID: 8647916, DOI: 10.1002/(sici)1097-4652(199607)168:1<199::aid-jcp24>3.0.co;2-1.Peer-Reviewed Original ResearchConceptsColony-stimulating factor-1CSF-1 expressionMC3T3-E1 cellsCSF-1 mRNACSF-1 gene transcriptionMRNA stability studiesFactor 1 geneNew protein synthesisSecond messenger pathwaysPrimary mouse osteoblastsColony-stimulating factor-1 (CSF-1) geneTranscriptional mechanismsGene transcriptionResponsive cell linesInduces transcriptionNorthern analysisProgenitor proliferationTNF-alpha treatmentProtein synthesisCAMP pathwayTranscript expressionMessenger pathwaysMurine osteoblastsCycloheximide treatmentCellular mechanisms
1994
Nocturnal hyperparathyroidism: a frequent feature of X-linked hypophosphatemia
Carpenter TO, Mitnick MA, Ellison A, Smith C, Insogna KL. Nocturnal hyperparathyroidism: a frequent feature of X-linked hypophosphatemia. The Journal Of Clinical Endocrinology & Metabolism 1994, 78: 1378-1383. PMID: 8200940, DOI: 10.1210/jcem.78.6.8200940.Peer-Reviewed Original ResearchConceptsHypophosphatemic ricketsFrequency of hyperparathyroidismMean iPTH valueNephrogenous cAMP excretionPathogenesis of nephrocalcinosisInitiation of therapyVitamin D preparationsGroup of patientsOnset of treatmentExaggerated secretionParathyroid statusIPTH valuesTertiary hyperparathyroidismParathyroid functionUntreated patientsOccasional complicationCAMP excretionHyperparathyroidismPhosphopenic ricketsD preparationsPatientsControl individualsRicketsIntact hormoneNocturnal rise
1992
Parathyroid hormone‐related protein stimulates prostaglandin E2 release from human osteoblast‐like cells: Modulating effect of peptide length
Mitnick M, Isales C, Paliwal I, Insogna K. Parathyroid hormone‐related protein stimulates prostaglandin E2 release from human osteoblast‐like cells: Modulating effect of peptide length. Journal Of Bone And Mineral Research 1992, 7: 887-896. PMID: 1332431, DOI: 10.1002/jbmr.5650070805.Peer-Reviewed Original ResearchConceptsCyclic AMP accumulationPGE2 releaseAMP accumulationCultured fetal rat long bonesFetal rat long bonesSaos-2 cellsBone resorptive actionProstaglandin E2 secretionBone-active cytokineHormone-related proteinPrimary human bone cell culturesRelease of PGE2Prostaglandin E2 releaseEffects of PTHrPRat long bonesForm of PTHrPHuman osteoblast-like cellsMalignancy syndromeE2 secretionHumoral hypercalcemiaBasal secretionPGE2 secretionE2 releaseOsteoblast-like cellsPTHrPSerum Parathyroid Hormone Level Is Elevated in Some Patients With Disorders of Keratinization
Milstone LM, Ellison AF, Insogna KL. Serum Parathyroid Hormone Level Is Elevated in Some Patients With Disorders of Keratinization. JAMA Dermatology 1992, 128: 926-930. PMID: 1320849, DOI: 10.1001/archderm.1992.01680170058005.Peer-Reviewed Original ResearchConceptsDisorders of keratinizationPityriasis rubra pilarisPTH levelsSecondary hyperparathyroidismPTH valuesSerum parathyroid hormone levelsNormal PTH valuesParathyroid hormone valuesSerum PTH valuesParathyroid hormone levelsSerum PTH levelsElevated PTH levelsHigh calcium dietIchthyosis linearis circumflexaCongenital ichthyosiform erythrodermaSecond patientBullous congenital ichthyosiform erythrodermaExact prevalenceCalcium metabolismHormone valuesHormone levelsPatientsActive PTHPhysiologic significanceDifferent disorders
1990
Treatment of humoral hypercalcemia of malignancy in rats with inhibitors of carbonic anhydrase
Brown G, Morris C, Mitnick M, Insogna K. Treatment of humoral hypercalcemia of malignancy in rats with inhibitors of carbonic anhydrase. Journal Of Bone And Mineral Research 1990, 5: 1037-1041. PMID: 1964358, DOI: 10.1002/jbmr.5650051007.Peer-Reviewed Original ResearchConceptsSerum calciumBone resorptionHumoral hypercalcemiaCarbonic anhydrase inhibitorsNephrogenous cyclic AMP excretionCyclic AMP excretionMean serum calciumOsteoclastic bone resorptionDrug-treated animalsLeydig cell tumorMechanism of actionUrine phosphorusUrine calciumSerum phosphorusCell tumorsAcetazolamide treatmentHypercalcemiaSignificant fallFisher ratsObserved hypercalcemiaResorptionDirect inhibitionSignificant riseMalignancyRats
1989
Native and a synthetic analogue of the malignancy-associated parathyroid hormone-like protein have in vitro transforming growth factor-like properties.
Insogna KL, Stewart AF, Morris CA, Hough LM, Milstone LM, Centrella M. Native and a synthetic analogue of the malignancy-associated parathyroid hormone-like protein have in vitro transforming growth factor-like properties. Journal Of Clinical Investigation 1989, 83: 1057-1060. PMID: 2537846, PMCID: PMC303783, DOI: 10.1172/jci113947.Peer-Reviewed Original ResearchConceptsGrowth factor-like propertiesParathyroid hormone-like proteinHormone-like proteinNRK-49F cellsParaneoplastic syndromeHumoral hypercalcemiaLatter hormoneHuman tumorsPhysiological actionsPTHHuman dermal fibroblastsSignificant increaseSoft agarDisplay actionsDermal fibroblastsSynthetic peptidesSynthetic analoguesHypercalcemiaMalignancySyndromeTumorsUnique primary structureSynthesis of a Gene Encoding Parathyroid Hormone-Like Protein-(1–141): Purification and Biological Characterization of the Expressed Protein*
THORIKAY M, KRAMER S, REYNOLDS F, SORVILLO J, DOESCHER L, WU T, MORRIS C, BURTIS W, INSOGNA K, VALENZUELA D, STEWART A. Synthesis of a Gene Encoding Parathyroid Hormone-Like Protein-(1–141): Purification and Biological Characterization of the Expressed Protein*. Endocrinology 1989, 124: 111-118. PMID: 2535801, DOI: 10.1210/endo-124-1-111.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBiological AssayBone and BonesCalciumCloning, MolecularCyclic AMPDNADogsEscherichia coliGenes, SyntheticKidneyMolecular Sequence DataMolecular WeightNeoplasm ProteinsOsteosarcomaParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsPlasmidsRatsRecombinant ProteinsTumor Cells, Cultured
1988
Humoral Hypercalcemia of Malignancy in Canine Lymphosarcoma*
Weir EC, Norrdin RW, Matus RE, Brooks MB, Broadus AE, Mitnick M, Johnston SD, Insogna KL. Humoral Hypercalcemia of Malignancy in Canine Lymphosarcoma*. Endocrinology 1988, 122: 602-608. PMID: 2828006, DOI: 10.1210/endo-122-2-602.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityHypercalcemic dogsCanine lymphosarcomaFractional phosphorus excretionEvidence of tumorQuantitative bone histomorphometryBone-resorbing factorsImmunoreactive PTH levelsPathogenesis of hypercalcemiaIliac crest biopsiesTumor tissue extractsCyclase-stimulating activityBone histomorphometric findingsCalcium excretionPTH levelsHumoral hypercalcemiaDihydroxyvitamin DBone resorptionHistomorphometric findingsBone histomorphometryPTH receptorBiopsy siteCrest biopsiesTumor extractsCompensatory increase
1987
Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*
WU T, INSOGNA K, HOUGH L, MILSTONE L, STEWART A. Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*. The Journal Of Clinical Endocrinology & Metabolism 1987, 65: 105-109. PMID: 3034948, DOI: 10.1210/jcem-65-1-105.Peer-Reviewed Original ResearchConceptsPTH-like factorHumoral hypercalcemiaPTH analogsPTH-responsive adenylate cyclaseDermal fibroblast linesAdenylate cyclaseTumor-derived factorsAdenylate cyclase responseDermal fibroblastsAdenylate cyclase stimulationHuman dermal fibroblastsM bovine PTHDermal physiologyFibroblast linesHuman dermal fibroblast linesBovine PTHHigh prevalenceTumor extractsCyclase responseSkin-derived fibroblastsCyclase stimulationPTHKeratinocyte-conditioned mediumHuman tumorsApparent potency
1986
Frequency and partial characterization of adenylate cyclase‐stimulating activity in tumors associated with humoral hypercalcemia of malignancy
Stewart A, Insogna K, Burtis W, Aminiafshar A, Wu T, Weir E, Broadus A. Frequency and partial characterization of adenylate cyclase‐stimulating activity in tumors associated with humoral hypercalcemia of malignancy. Journal Of Bone And Mineral Research 1986, 1: 267-276. PMID: 2845726, DOI: 10.1002/jbmr.5650010305.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityCyclase-stimulating activityHumoral hypercalcemiaTumor extractsSyndrome of HHMBone-resorbing activityNonmalignant tissue samplesControl tumorsHumoral factorsControl groupTumorsTissue samplesHypercalcemiaPatientsMalignancyTissue culture mediumHigh-performance liquid chromatographyHHMLarge groupCulture mediumLiquid chromatographyGroupSyndromeActivityA Parathyroid Hormone-Like Protein from Cultured Human Keratinocytes
Merendino JJ, Insogna KL, Milstone LM, Broadus AE, Stewart AF. A Parathyroid Hormone-Like Protein from Cultured Human Keratinocytes. Science 1986, 231: 388-390. PMID: 2417317, DOI: 10.1126/science.2417317.Peer-Reviewed Original ResearchConceptsHumoral hypercalcemiaParathyroid hormoneHuman keratinocytesParathyroid hormone receptor agonistsParathyroid hormone-like proteinSquamous epithelial originExtracts of tumorsHormone receptor agonistsHormone-like proteinHormone-like factorsAdenylate cyclase activityRat osteosarcoma cellsAdenylate cyclase productionNative parathyroid hormoneCultured human keratinocytesNormal human keratinocytesReceptor agonistMalignant tumorsCyclic adenosine monophosphateHypercalcemiaEpithelial originKeratinocyte-conditioned mediumMalignancyCyclase activityCyclase productionNephrogenous Cyclic AMP, Adenylate Cyclase-Stimulating Activity, and the Humoral Hypercalcemia of Malignancy
GODSALL JW, BURTIS WJ, INSOGNA KL, BROADUS AE, STEWART AF. Nephrogenous Cyclic AMP, Adenylate Cyclase-Stimulating Activity, and the Humoral Hypercalcemia of Malignancy. 1986, 42: 705-750. PMID: 3016837, DOI: 10.1016/b978-0-12-571142-5.50021-5.BooksConceptsNephrogenous cyclic AMPCyclase-stimulating activityUcAMP excretionHumoral hypercalcemiaLocal osteolytic hypercalcemiaAdenylate cyclase-stimulating activityBone-resorbing activityCyclic AMPHPT groupNcAMP excretionNormocalcemic patientsPathophysiologic syndromeTumor extractsAnimal modelsHypercalcemiaAnimal tumorsTumorsNcAMPHHMExcretionPatientsMalignancyObserved elevationBasic proteinAMP
1985
Sensitivity of the Parathyroid Hormone–1, 25-Dihydroxyvitamin D Axis to Variations in Calcium Intake in Patients with Primary Hyperparathyroidism
Insogna K, Mitnick M, Stewart A, Burtis W, Mallette L, Broadus A. Sensitivity of the Parathyroid Hormone–1, 25-Dihydroxyvitamin D Axis to Variations in Calcium Intake in Patients with Primary Hyperparathyroidism. New England Journal Of Medicine 1985, 313: 1126-1130. PMID: 2995810, DOI: 10.1056/nejm198510313131805.Peer-Reviewed Original ResearchConceptsPrimary hyperparathyroidismParathyroid functionParathyroid hormone-vitamin D axisAutonomous parathyroid functionNephrogenous cyclic AMPImmunoreactive parathyroid hormoneNormal dietary intakeExtracellular calcium concentrationParathyroid hormone 1Calcium excretionCalcium intakeUnselected patientsSerum levelsParathyroid hormoneDihydroxyvitamin DDietary calciumPlasma levelsDietary intakePhysiologic amountsHyperparathyroidismPatientsHormone 1Calcium concentrationCyclic AMPSignificant reductionElevated Production Rate of 1,25-Dihydroxyvitamin D in Patients with Absorptive Hypercalciuria*
INSOGNA K, BROADUS A, DREYER B, ELLISON A, GERTNER J. Elevated Production Rate of 1,25-Dihydroxyvitamin D in Patients with Absorptive Hypercalciuria*. The Journal Of Clinical Endocrinology & Metabolism 1985, 61: 490-495. PMID: 2991323, DOI: 10.1210/jcem-61-3-490.Peer-Reviewed Original ResearchConceptsAbsorptive hypercalciuriaMicrograms/dayNormal subjectsMetabolic clearancePlasma radioactivityMean steady-state concentrationMCR valuesHypercalciuric patientsCalcium intakeDihydroxyvitamin DSerial measurementsHypercalciuriaPatientsConstant infusionConflicting resultsClearanceSteady-state concentrations
1984
Evidence for Disordered Control of 1,25-Dihydroxyvitamin D Production in Absorptive Hypercalciuria
Broadus A, Insogna K, Lang R, Ellison A, Dreyer B. Evidence for Disordered Control of 1,25-Dihydroxyvitamin D Production in Absorptive Hypercalciuria. New England Journal Of Medicine 1984, 311: 73-80. PMID: 6330548, DOI: 10.1056/nejm198407123110201.Peer-Reviewed Original ResearchConceptsAbsorptive hypercalciuriaCalcium intakeDietary calcium intakeDihydroxyvitamin D productionRenal phosphate handlingDihydroxyvitamin DPhosphate handlingSuppression testHypercalciuriaPatientsD productionShort-term increaseSyndromeIntakePresent studyInitial levelLarge majorityPrevious studiesEvidenceAbnormalitiesWeeks
1983
Impaired phosphorus conservation and 1,25 dihydroxyvitamin D generation during phosphorus deprivation in familial hypophosphatemic rickets.
Insogna KL, Broadus AE, Gertner JM. Impaired phosphorus conservation and 1,25 dihydroxyvitamin D generation during phosphorus deprivation in familial hypophosphatemic rickets. Journal Of Clinical Investigation 1983, 71: 1562-1569. PMID: 6306051, PMCID: PMC370362, DOI: 10.1172/jci110912.Peer-Reviewed Original ResearchConceptsFamilial hypophosphatemic ricketsNormal subjectsHypophosphatemic ricketsImmunoreactive parathyroid hormone levelsD plasma levelsNephrogenous cyclic AMPParathyroid hormone levelsGlomerular filtration rateDietary phosphorus deprivationHealthy male volunteersTubular reabsorptive capacityLow phosphorus dietCyclic AMP measurementsMale patientsRenal responseFiltration ratePlasma levelsHormone levelsAbnormal responseMale volunteersReabsorptive capacityPatientsPhosphorus intakePhosphorus dietPhosphate conservation
1982
Influence of pregnancy on immunoreactive parathyroid hormone levels
Gillette M, Insogna K, Lewis A, Baran D. Influence of pregnancy on immunoreactive parathyroid hormone levels. Calcified Tissue International 1982, 34: 9-12. PMID: 6279256, DOI: 10.1007/bf02411200.Peer-Reviewed Original ResearchConceptsImmunoreactive PTHTubular maximum phosphate reabsorptionImmunoreactive parathyroid hormone levelsAge-matched female controlsHealthy pregnant patientsNephrogenous cAMP excretionParathyroid hormone metabolismHealthy age-matched female controlsParathyroid hormone levelsInfluence of pregnancySignificant increaseBiological effectsIPTH levelsPregnant patientsCreatinine clearanceUrinary calciumCreatinine ratioPTH metabolismSerum calciumSerum phosphorusThird trimesterVitamin DBlood levelsBone resorptionPhosphate reabsorption
1981
Effect of Age on Serum Immunoreactive Parathyroid Hormone and Its Biological Effects*
INSOGNA K, LEWIS A, LIPINSKI B, BRYANT C, BARAN D. Effect of Age on Serum Immunoreactive Parathyroid Hormone and Its Biological Effects*. The Journal Of Clinical Endocrinology & Metabolism 1981, 53: 1072-1075. PMID: 6270181, DOI: 10.1210/jcem-53-5-1072.Peer-Reviewed Original ResearchConceptsIPTH levelsElderly subjectsTubular maximum phosphate reabsorptionImmunoreactive parathyroid hormone levelsSerum immunoreactive parathyroid hormoneImmunoreactive parathyroid hormoneParathyroid hormone levelsBiological effectsCreatinine clearanceEffect of ageNephrogenous cAMPUrinary calciumCreatinine ratioSerum phosphateParathyroid hormoneBone lossCalcium ingestionBone resorptionPhosphate reabsorptionHormone levelsHealthy volunteersSignificant negative correlationSignificant positive correlationIPTHOlder volunteers