Ammonia Channeling in Plasmodium falciparum GMP Synthetase: Investigation by NMR Spectroscopy and Biochemical Assays
Bhat JY, Venkatachala R, Singh K, Gupta K, Sarma SP, Balaram H. Ammonia Channeling in Plasmodium falciparum GMP Synthetase: Investigation by NMR Spectroscopy and Biochemical Assays. Biochemistry 2011, 50: 3346-3356. PMID: 21413787, DOI: 10.1021/bi1017057.Peer-Reviewed Original ResearchConceptsGMP synthetasePlasmodium falciparum GMP synthetaseBiochemical assaysPurine biosynthetic pathwayAmmonia channelingGlutamine amidotransferasesGlutaminase domainMolecular tunnelBiosynthetic pathwayCatalytic residuesGlutaminase siteSeparate active sitesExternal mediumThioester intermediateExternal ammoniaCys-102NMR spectroscopyConcentration of glutamineSynthetaseUncompetitive inhibitorGlutamineActive siteSynthetase siteProton NMR spectroscopyAssays