2021
Tet2 Controls the Responses of β cells to Inflammation in Autoimmune Diabetes
Rui J, Deng S, Perdigoto AL, Ponath G, Kursawe R, Lawlor N, Sumida T, Levine-Ritterman M, Stitzel ML, Pitt D, Lu J, Herold KC. Tet2 Controls the Responses of β cells to Inflammation in Autoimmune Diabetes. Nature Communications 2021, 12: 5074. PMID: 34417463, PMCID: PMC8379260, DOI: 10.1038/s41467-021-25367-z.Peer-Reviewed Original ResearchConceptsImmune cellsΒ-cellsNOD/SCID recipientsDiabetogenic immune cellsDiabetogenic T cellsBone marrow transplantType 1 diabetesExpression of TET2Human β-cellsIslet infiltratesSCID recipientsMarrow transplantInflammatory pathwaysTransfer of diseaseT cellsInflammatory genesImmune killingPathologic interactionsReduced expressionDiabetesInflammationTET2MiceRecipientsCells
2015
microRNA Expression Profiling: Technologies, Insights, and Prospects
Roden C, Mastriano S, Wang N, Lu J. microRNA Expression Profiling: Technologies, Insights, and Prospects. Advances In Experimental Medicine And Biology 2015, 888: 409-421. PMID: 26663195, DOI: 10.1007/978-3-319-22671-2_21.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell Line, TumorDisease Models, AnimalGene Expression ProfilingGene Expression Regulation, NeoplasticHigh-Throughput Nucleotide SequencingHumansMicroRNAsMolecular Sequence DataNeoplasmsReverse Transcriptase Polymerase Chain ReactionSequence Homology, Nucleic AcidSignal TransductionConceptsLong small noncoding RNAsExpression profilingMiRNA isoformsMiRNA expressionProfiling technologiesDiverse biological processesSingle-cell variabilitySmall noncoding RNAsMiRNA profiling technologiesGlobal miRNA expressionNext-generation sequencingNoncoding RNAsCell variabilitySingle-molecule measurementsBiological functionsBiological processesTumor suppressorMicroRNA researchQuantitative RT-PCRCareful experimental designMiRNAsIsoformsRT-PCRProfilingExpression
2012
miR-196b directly targets both HOXA9/MEIS1 oncogenes and FAS tumour suppressor in MLL-rearranged leukaemia
Li Z, Huang H, Chen P, He M, Li Y, Arnovitz S, Jiang X, He C, Hyjek E, Zhang J, Zhang Z, Elkahloun A, Cao D, Shen C, Wunderlich M, Wang Y, Neilly MB, Jin J, Wei M, Lu J, Valk PJ, Delwel R, Lowenberg B, Le Beau MM, Vardiman J, Mulloy JC, Zeleznik-Le NJ, Liu PP, Zhang J, Chen J. miR-196b directly targets both HOXA9/MEIS1 oncogenes and FAS tumour suppressor in MLL-rearranged leukaemia. Nature Communications 2012, 3: 688. PMID: 22353710, PMCID: PMC3514459, DOI: 10.1038/ncomms1681.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBase SequenceCell Transformation, NeoplasticCells, CulturedFas ReceptorFemaleGene Expression Regulation, NeoplasticGenes, Tumor SuppressorHematopoiesisHomeodomain ProteinsHumansLeukemia, Myeloid, AcuteMaleMiceMice, Inbred C57BLMicroRNAsMyeloid Ecotropic Viral Integration Site 1 ProteinMyeloid-Lymphoid Leukemia ProteinNeoplasm ProteinsSequence Analysis, DNAConceptsMiR-196bTumor suppressorMiRNA regulation mechanismOverexpression of FASBone marrow transplantationEssential oncogenic roleMiRNA regulationEctopic expressionMixed lineage leukemiaMEIS1 expressionMLL fusionsProapoptotic genesSingle miRNACell differentiationDirect targetLeukaemic phenotypeHoxa9/Meis1Marrow transplantationNormal developmentFurther repressionLeukaemic cellsOncogenic roleLineage leukemiaNormal haematopoiesisSecondary transplantation
2009
Regulation of mir-196b by MLL and its overexpression by MLL fusions contributes to immortalization
Popovic R, Riesbeck LE, Velu CS, Chaubey A, Zhang J, Achille NJ, Erfurth FE, Eaton K, Lu J, Grimes HL, Chen J, Rowley JD, Zeleznik-Le NJ. Regulation of mir-196b by MLL and its overexpression by MLL fusions contributes to immortalization. Blood 2009, 113: 3314-3322. PMID: 19188669, PMCID: PMC2665896, DOI: 10.1182/blood-2008-04-154310.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DifferentiationCell ProliferationCell Transformation, NeoplasticCells, CulturedEmbryonic Stem CellsGene Expression RegulationHistone-Lysine N-MethyltransferaseLeukemiaMiceMice, Inbred C57BLMicroRNAsMolecular Sequence DataMyeloid-Lymphoid Leukemia ProteinRecombinant Fusion ProteinsSequence Homology, Nucleic AcidUp-RegulationConceptsMLL fusion proteinsHox genesMiR-196bLeukemogenic MLL fusion proteinsFusion proteinEmbryonic stem cell differentiationStem cell differentiationDifferentiated hematopoietic cellsShort-term hematopoietic stem cellsMixed lineage leukemia (MLL) geneBone marrow progenitor cellsLeukemia developmentHOXA clusterHematopoietic stem cellsPrimary leukemia samplesChimeric proteinMarrow progenitor cellsHematopoietic lineagesCell differentiationLeukemia geneFusion contributesChromosomal translocationsHematopoietic cellsGenesStem cells
2008
Dicer-dependent pathways regulate chondrocyte proliferation and differentiation
Kobayashi T, Lu J, Cobb BS, Rodda SJ, McMahon AP, Schipani E, Merkenschlager M, Kronenberg HM. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 1949-1954. PMID: 18238902, PMCID: PMC2538863, DOI: 10.1073/pnas.0707900105.Peer-Reviewed Original ResearchConceptsDicer-dependent pathwaysSkeletal developmentBiogenesis of miRNAsMiRNA target genesMammalian skeletal developmentSmall noncoding RNAsSkeletal growth defectsChondrocyte proliferationSuppress gene expressionNormal skeletal developmentDiverse organismsCritical roleGrowth defectNoncoding RNAsRNA abundanceGene expressionExpression changesMicroarray analysisBiological processesSignaling systemMiRNAsBase pairingDistinct mechanismsIhh-PTHrPHypertrophic chondrocytes
2006
Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature
Hazony Y, Lu J, St. Hilaire C, Ravid K. Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature. Nucleic Acids Research 2006, 34: 4416-4428. PMID: 16936310, PMCID: PMC1636359, DOI: 10.1093/nar/gkl578.Peer-Reviewed Original ResearchConceptsCell-specific gene expressionNon-coding regionsRelated DNA sequencesDNA sequencesGene expressionMegakaryocytic lineageUnique transcription factorsCommon DNA sequenceNon-coding sequencesGroup of genesGene promoter sequencesMammalian genomesRegulatory sequencesTranscription factorsPromoter sequencesCross-species differencesGene promoterDNA signaturesLineagesSpecific sequencesCommon sequenceDNA samplesSequencePromoter groupExpression
2005
Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals
Xie X, Lu J, Kulbokas EJ, Golub TR, Mootha V, Lindblad-Toh K, Lander ES, Kellis M. Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals. Nature 2005, 434: 338-345. PMID: 15735639, PMCID: PMC2923337, DOI: 10.1038/nature03441.Peer-Reviewed Original ResearchConceptsMiRNA genesRegulatory motifsNew miRNA genesHuman miRNA genesPost-transcriptional regulationCommon regulatory motifsLikely target genesMammalian genomesDog genomeGene regulationPromoter analysisHuman genomeHuman genesHuman promotersTarget genesUntranslated regionSystematic discoveryGenomeComprehensive identificationGenesNew motifMotifCandidate motifsFunctional elementsPromoter
1999
Rat NAP1: cDNA cloning and upregulation by Mpl ligand
Cataldo L, Zhang Y, Lu J, Ravid K. Rat NAP1: cDNA cloning and upregulation by Mpl ligand. Gene 1999, 226: 355-364. PMID: 9931510, DOI: 10.1016/s0378-1119(98)00563-0.Peer-Reviewed Original ResearchMeSH KeywordsAgingAmino Acid SequenceAnimalsBase SequenceBone Marrow CellsCell Cycle ProteinsCell LineageCells, CulturedCloning, MolecularDNA, ComplementaryGene Expression RegulationMolecular Sequence DataNuclear ProteinsNucleosome Assembly Protein 1ProteinsRatsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSequence Homology, Amino AcidThrombopoietinUp-RegulationConceptsMpl ligandNucleosome assembly proteinPrimary bone marrow cellsFull-length cDNASize classesFormation of nucleosomesC-Mpl receptorChromatin reorganizationMultiple size classesAssembly proteinPolymerase chain reaction approachRelaxed circular DNACDNA cloningKb cDNAHematopoietic lineagesKb mRNARecombinant proteinsHematopoietic cytokinesHematopoietic cellsCircular DNAPlatelet precursorsBone marrow cellsHematopoietic tissuesCell linesCDNA