2013
The IGF2 intronic miR-483 selectively enhances transcription from IGF2 fetal promoters and enhances tumorigenesis
Liu M, Roth A, Yu M, Morris R, Bersani F, Rivera MN, Lu J, Shioda T, Vasudevan S, Ramaswamy S, Maheswaran S, Diederichs S, Haber DA. The IGF2 intronic miR-483 selectively enhances transcription from IGF2 fetal promoters and enhances tumorigenesis. Genes & Development 2013, 27: 2543-2548. PMID: 24298054, PMCID: PMC3861668, DOI: 10.1101/gad.224170.113.Peer-Reviewed Original ResearchConceptsInsulin-like growth factor 2Loss of imprintingUntranslated regionFunctional positive feedback loopRNA helicase DHX9IGF2 mRNAAdditional regulatory mechanismsIntronic miRNANuclear poolHost genesEctopic expressionMicroRNA screenPositive feedback loopIGF2 transcriptionPrimary Wilms tumorsFetal promotersIGF2 transcriptsPromoter contributesRegulatory mechanismsIGF2 geneGrowth factor 2IGF2 expressionTranscriptionGenesMiR-483
2010
Lineage-Specific Transcriptional Regulation of DICER by MITF in Melanocytes
Levy C, Khaled M, Robinson KC, Veguilla RA, Chen PH, Yokoyama S, Makino E, Lu J, Larue L, Beermann F, Chin L, Bosenberg M, Song JS, Fisher DE. Lineage-Specific Transcriptional Regulation of DICER by MITF in Melanocytes. Cell 2010, 141: 994-1005. PMID: 20550935, PMCID: PMC2897150, DOI: 10.1016/j.cell.2010.05.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosis Regulatory ProteinsBcl-2-Like Protein 11Cell DifferentiationCell SurvivalCells, CulturedEpidermal CellsGene Expression RegulationGene Knockdown TechniquesHair FollicleHumansMelanocytesMembrane ProteinsMiceMice, Inbred C57BLMicrophthalmia-Associated Transcription FactorMicroRNAsPromoter Regions, GeneticProto-Oncogene ProteinsRibonuclease IIITranscription, GeneticUp-RegulationConceptsTranscriptional start siteLineage-specific transcriptional regulationDicer-dependent processingRegulatory element upstreamMITF bindsTranscriptional regulationMature miRNAsProapoptotic regulatorsMiRNA regulationStart siteMelanocyte survivalCentral regulatorDicerMelanocyte differentiationElement upstreamMiRNA expressionCell typesDicer expressionMiRNAsTranscriptional targetingMITFRegulatorMelanocytesExpressionRegulation
2006
Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature
Hazony Y, Lu J, St. Hilaire C, Ravid K. Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature. Nucleic Acids Research 2006, 34: 4416-4428. PMID: 16936310, PMCID: PMC1636359, DOI: 10.1093/nar/gkl578.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBinding SitesComputational BiologyHumansMegakaryocytesMiceMolecular Sequence DataPromoter Regions, GeneticSequence Analysis, DNASoftwareTranscription FactorsConceptsCell-specific gene expressionNon-coding regionsRelated DNA sequencesDNA sequencesGene expressionMegakaryocytic lineageUnique transcription factorsCommon DNA sequenceNon-coding sequencesGroup of genesGene promoter sequencesMammalian genomesRegulatory sequencesTranscription factorsPromoter sequencesCross-species differencesGene promoterDNA signaturesLineagesSpecific sequencesCommon sequenceDNA samplesSequencePromoter groupExpression
2005
Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals
Xie X, Lu J, Kulbokas EJ, Golub TR, Mootha V, Lindblad-Toh K, Lander ES, Kellis M. Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals. Nature 2005, 434: 338-345. PMID: 15735639, PMCID: PMC2923337, DOI: 10.1038/nature03441.Peer-Reviewed Original ResearchConceptsMiRNA genesRegulatory motifsNew miRNA genesHuman miRNA genesPost-transcriptional regulationCommon regulatory motifsLikely target genesMammalian genomesDog genomeGene regulationPromoter analysisHuman genomeHuman genesHuman promotersTarget genesUntranslated regionSystematic discoveryGenomeComprehensive identificationGenesNew motifMotifCandidate motifsFunctional elementsPromoter
2004
Properties of Ets-1 Binding to Chromatin and Its Effect on Platelet Factor 4 Gene Expression
Lu J, Pazin MJ, Ravid K. Properties of Ets-1 Binding to Chromatin and Its Effect on Platelet Factor 4 Gene Expression. Molecular And Cellular Biology 2004, 24: 428-441. PMID: 14673175, PMCID: PMC303331, DOI: 10.1128/mcb.24.1.428-441.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsChromatinDetergentsGene Expression RegulationHumansMegakaryocytesMiceNucleosomesPlatelet Factor 4Platelet Glycoprotein GPIb-IX ComplexPlatelet Membrane GlycoproteinsPromoter Regions, GeneticProto-Oncogene Protein c-ets-1Proto-Oncogene ProteinsProto-Oncogene Proteins c-etsRatsSarcosineTranscription FactorsConceptsEts-1 bindsPromoter activityLineage-specific factorsChromatin immunoprecipitation assaysEts-1 siteGlycoprotein IIbLineage-specific promotersNaked DNAGPIIb promoterTranscriptional regulationAccessory factorsPF4 promoterTranscription factorsHypersensitive sitesImmunoprecipitation assaysHematopoietic lineagesDNase IChromatinGene expressionPromoter activationPromoterProximal regionModel systemFactor 4ET-1
2002
A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation
Kaluzhny Y, Hechler B, Lu J, Nguyen HG, Cataldo LM, Ravid K. A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation. FEBS Letters 2002, 527: 279-283. PMID: 12220674, DOI: 10.1016/s0014-5793(02)03230-1.Peer-Reviewed Original Research3' Flanking Region3' Untranslated RegionsAnimalsCell DifferentiationCells, CulturedGlyceraldehyde-3-Phosphate DehydrogenasesMegakaryocytesMiceNeoplasm ProteinsPlatelet Factor 4Promoter Regions, GeneticProto-Oncogene ProteinsReceptors, CytokineReceptors, Purinergic P2Receptors, Purinergic P2Y1Receptors, ThrombopoietinRNA StabilityRNA, MessengerThrombopoietin
1999
Mpl ligand enhances the transcription of the cyclin D3 gene: a potential role for Sp1 transcription factor.
Wang Z, Zhang Y, Lu J, Sun S, Ravid K. Mpl ligand enhances the transcription of the cyclin D3 gene: a potential role for Sp1 transcription factor. Blood 1999, 93: 4208-21. PMID: 10361118, DOI: 10.1182/blood.v93.12.4208.412k17_4208_4221.Peer-Reviewed Original ResearchConceptsProtein phosphatase 1Cyclin D3 promoterMpl ligandCyclin D3 geneTranscription factorsSp1-dependent genesD3 geneSp1 transcription factorForm of Sp1Basal promoter activityMegakaryocytic cell linesCyclin D3 proteinSp familySp1 proteinD3 gene expressionSp1 sitesPhosphatase 1Okadaic acidCyclin D3 gene expressionNuclear runSp1DNase IGene expressionPromoter activityPromoter region