2017
Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate
Wang N, Cheng J, Fan R, Lu J. Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate. Analyst 2017, 142: 3203-3211. PMID: 28765841, PMCID: PMC5605290, DOI: 10.1039/c7an00670e.Peer-Reviewed Original ResearchConceptsWhole cell lysatesSmall non-coding RNAsComplex regulatory networkMiRNA profilingCell lysatesPost-transcriptional levelIsogenic cell linesNon-coding RNAsAdaptor ligationLow quantity samplesGenome scaleRegulatory networksGlobal profilingMiRNA captureGene expressionExpression profilesHuman diseasesMiRNA expressionLibrary preparationMiRNA alterationsCell typesMiRNA releaseRNA purificationMulti-step purificationCell lines
2008
Distinct microRNA expression profiles in acute myeloid leukemia with common translocations
Li Z, Lu J, Sun M, Mi S, Zhang H, Luo RT, Chen P, Wang Y, Yan M, Qian Z, Neilly MB, Jin J, Zhang Y, Bohlander SK, Zhang DE, Larson RA, Le Beau MM, Thirman MJ, Golub TR, Rowley JD, Chen J. Distinct microRNA expression profiles in acute myeloid leukemia with common translocations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 15535-15540. PMID: 18832181, PMCID: PMC2563085, DOI: 10.1073/pnas.0808266105.Peer-Reviewed Original ResearchConceptsPolo-like kinase 2Genome-wide miRNA expression analysisDistinct miRNA expression patternsMiRs-126/126Normal bone marrow progenitor cellsMiRNA expression patternsCommon translocationBone marrow progenitor cellsGenomic lociMiRNA expression analysisExpression analysisMarrow progenitor cellsAcute myeloid leukemia samplesExpression patternsSpecific miRNAsKinase 2Expression profilesTumor suppressorFunction experimentsGenetic rearrangementsPromoter demethylationColony-forming abilityImportant regulatorAML1-ETOExpression signatures
2007
MicroRNA Expression Profiles in Acute Myeloid Leukemia with Common Translocations.
Li Z, Lu J, Sun M, Mi S, Zhang H, Luo R, Qian Z, Neilly M, Wang Y, Jin J, Zhang Y, Bohlander S, Larson R, LeBeau M, Thirman M, Golub T, Rowley J, Chen J. MicroRNA Expression Profiles in Acute Myeloid Leukemia with Common Translocations. Blood 2007, 110: 3181. DOI: 10.1182/blood.v110.11.3181.3181.Peer-Reviewed Original ResearchAcute myeloid leukemiaTarget genesExpression profilesDNA copy number amplificationsProtein-coding genesExpression profiling methodsImportant target genesMyeloid leukemiaNon-coding RNAsCritical target genesNormal controlsTreatment of AMLCBF acute myeloid leukemiaCopy number amplificationMiRNA expression profilesDevelopment of AMLCommon translocationEpigenetic regulationMicroRNA expression profileWide analysisComplex genetic alterationsExpression profilingDNA microarraysBiological roleTumor suppressorAltered microRNA expression in human heart disease
Ikeda S, Kong SW, Lu J, Bisping E, Zhang H, Allen PD, Golub TR, Pieske B, Pu WT. Altered microRNA expression in human heart disease. Physiological Genomics 2007, 31: 367-373. PMID: 17712037, DOI: 10.1152/physiolgenomics.00144.2007.Peer-Reviewed Original ResearchConceptsHeart diseaseDiagnostic groupsHuman heart failureLeft ventricular samplesHeart disease pathogenesisHeart failureIschemic cardiomyopathyDisease groupHeart functionDisease pathogenesisExpression profilesControl groupHuman heart diseaseVentricular samplesClinical diagnosisHuman left ventricular samplesContribution of microRNAsDiseaseFurther studiesImportant regulatorMultiple testingMiRNA expressionMicroRNAsExpressionGroup
2005
MicroRNA expression profiles classify human cancers
Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, Downing JR, Jacks T, Horvitz HR, Golub TR. MicroRNA expression profiles classify human cancers. Nature 2005, 435: 834-838. PMID: 15944708, DOI: 10.1038/nature03702.Peer-Reviewed Original ResearchConceptsCluster of microRNAsHuman cancersHuman chromosome 13Non-coding RNAsImportant transcription factorMicroRNA expression profileTranscription factorsExpression profilesGlobal downregulationRegulatory moleculesDifferentiation statePotential oncogeneMiR-17C-MycChromosome 13MicroRNAsHuman B-cell lymphomasMicroRNA profilesTumor formationVivo modelRNANucleotidesOncogeneDownregulationExpression