2019
Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock
Duarte-Neto A, Croda J, Pagliari C, Soriano F, Nicodemo A, Duarte M. Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock. Frontiers In Immunology 2019, 10: 920. PMID: 31114579, PMCID: PMC6503108, DOI: 10.3389/fimmu.2019.00920.Peer-Reviewed Original ResearchConceptsSeptic shockLeptospirosis patientsSevere leptospirosisPulmonary hemorrhagePositive cellsRed pulpBacterial septic shockSeptic shock patientsCaspase-3-positive cellsImmune cell markersSpleen of patientsActive caspase-3 positive cellsSemi-quantitative scoreImmunomodulatory treatmentShock patientsCellular immunosuppressionIL-10Immunologic featuresImmunosuppressive stateMarked atrophyEndothelial activationControl spleensHistological featuresIntense infiltrationPlasma cells
2011
The Multifunctional LigB Adhesin Binds Homeostatic Proteins with Potential Roles in Cutaneous Infection by Pathogenic Leptospira interrogans
Choy HA, Kelley MM, Croda J, Matsunaga J, Babbitt JT, Ko AI, Picardeau M, Haake DA. The Multifunctional LigB Adhesin Binds Homeostatic Proteins with Potential Roles in Cutaneous Infection by Pathogenic Leptospira interrogans. PLOS ONE 2011, 6: e16879. PMID: 21347378, PMCID: PMC3036719, DOI: 10.1371/journal.pone.0016879.Peer-Reviewed Original Research
2009
Leptospirosis pulmonary haemorrhage syndrome is associated with linear deposition of immunoglobulin and complement on the alveolar surface
Croda J, Neto A, Brasil R, Pagliari C, Nicodemo A, Duarte M. Leptospirosis pulmonary haemorrhage syndrome is associated with linear deposition of immunoglobulin and complement on the alveolar surface. Clinical Microbiology And Infection 2009, 16: 593-599. PMID: 19778300, DOI: 10.1111/j.1469-0691.2009.02916.x.Peer-Reviewed Original ResearchConceptsLeptospirosis pulmonary hemorrhage syndromePulmonary hemorrhage syndromeLPHS patientsPulmonary hemorrhageAlveolar surfaceLinear depositionUnique histological patternLife-threatening syndromeImmunoglobulin depositsTreatment regimensPathological featuresPresent studyHistological patternLung specimensLung tissueSevere diseaseAlveolar septaHuman leptospirosisLinear stainingSyndromeZoonotic infectionGranular stainingHemorrhageHeterogeneous stainingPatients
2008
Genome-Wide Transposon Mutagenesis in Pathogenic Leptospira Species
Murray GL, Morel V, Cerqueira GM, Croda J, Srikram A, Henry R, Ko AI, Dellagostin OA, Bulach DM, Sermswan RW, Adler B, Picardeau M. Genome-Wide Transposon Mutagenesis in Pathogenic Leptospira Species. Infection And Immunity 2008, 77: 810-816. PMID: 19047402, PMCID: PMC2632054, DOI: 10.1128/iai.01293-08.Peer-Reviewed Original ResearchConceptsTransposon mutagenesisTransposon mutantsGenome-wide transposon mutagenesisL. interrogansTransposon insertion sitesNovel virulence factorsEssential genesHypothetical genesGene functionGenome sequenceTransposon insertionGene disruptionGenetic manipulationDifferent genesGenetic analysisHeme biosynthesisPathogenic Leptospira speciesMutantsSequence analysisGenesVirulence factorsInsertional hot spotsLocation of insertionGenomeMutagenesisTargeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis
Croda J, Figueira CP, Wunder EA, Santos CS, Reis MG, Ko AI, Picardeau M. Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis. Infection And Immunity 2008, 76: 5826-5833. PMID: 18809657, PMCID: PMC2583567, DOI: 10.1128/iai.00989-08.Peer-Reviewed Original ResearchConceptsPathogenic Leptospira speciesLeptospira speciesAcute disease manifestationsPersistent renal colonizationInoculation of ratsDisease manifestationsHamster modelRenal colonizationPathogenic mechanismsAnimal modelsPersistent colonizationDecrease virulenceLeptospira interrogansHomologous recombinationLeptospirosisVirulence factorsBacterial adherenceImmunoblot analysisCorresponding chromosomal locusWild-type strainCultured cellsFirst evidenceSpectinomycin resistance geneRepeat proteinsTargeted Mutagenesis
2007
The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis
Silva É, Medeiros MA, McBride AJ, Matsunaga J, Esteves GS, Ramos JG, Santos CS, Croda J, Homma A, Dellagostin OA, Haake DA, Reis MG, Ko AI. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine 2007, 25: 6277-6286. PMID: 17629368, PMCID: PMC1994161, DOI: 10.1016/j.vaccine.2007.05.053.Peer-Reviewed Original ResearchConceptsMajor public health problemRobust antibody responseGolden Syrian hamstersPublic health problemPotential intervention strategiesLethal inoculumSurface-exposed determinantsProtective immunityAntibody responseSterilizing immunityLethal infectionHamster modelVaccine candidatesSubunit vaccineCompanion animalsHealth problemsVeterinary diseasesSyrian hamstersLeptospiral immunoglobulin-like (Lig) proteinsLeptospira interrogansLeptospirosisL. interrogansIntervention strategiesVirulence factorsImmunoglobulin-like proteinLeptospira Immunoglobulin-Like Proteins as a Serodiagnostic Marker for Acute Leptospirosis
Croda J, Ramos JG, Matsunaga J, Queiroz A, Homma A, Riley LW, Haake DA, Reis MG, Ko AI. Leptospira Immunoglobulin-Like Proteins as a Serodiagnostic Marker for Acute Leptospirosis. Journal Of Clinical Microbiology 2007, 45: 1528-1534. PMID: 17360842, PMCID: PMC1865864, DOI: 10.1128/jcm.02344-06.Peer-Reviewed Original ResearchConceptsSerodiagnostic markerDays of illnessLarge disease burdenLeptospira immunoglobulin-like proteinAcute infectionClinical presentationAcute leptospirosisIgM antibodiesAntibody responseDisease burdenPutative virulence determinantsIgM enzymeTimely diagnosisSevere formHealthy individualsMicroagglutination testPatientsUrban outbreaksHigh mortalityInfectious diseasesPathogenic serovarsLeptospirosisImmunosorbent assaySerumSpecific markersMonoclonal Antibodies Against LipL32, The Major Outer Membrane Protein of Pathogenic Leptospira Production, Characterization, and Testing in Diagnostic Applications
Fernandes C, Seixas FK, Coutinho ML, Vasconcellos F, Seyffert N, Croda J, McBride AJ, Ko AI, Dellagostin OA, Aleixo J. Monoclonal Antibodies Against LipL32, The Major Outer Membrane Protein of Pathogenic Leptospira Production, Characterization, and Testing in Diagnostic Applications. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 2007, 26: 35-41. PMID: 17316084, DOI: 10.1089/hyb.2006.033.Peer-Reviewed Original ResearchConceptsMembrane proteinsNative proteinMajor Outer Membrane ProteinOuter membrane lipoproteinOuter Membrane ProteinOuter membrane proteinsOuter membraneMembrane lipoproteinWide antigenic diversityBacterial cellsLeptospiral cellsIntact bacteriaProteinProtein enzyme-linked immunosorbent assayAntigenic diversityPolymerase chain reaction amplificationHeat-treated cellsMonoclonal antibodiesRecombinant formChain reaction amplificationEnzyme-linked immunosorbent assayFluorescein isothiocyanateCellsMurine monoclonal antibodiesPathogenic Leptospira
2001
Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans
Guerreiro H, Croda J, Flannery B, Mazel M, Matsunaga J, Reis M, Levett P, Ko A, Haake D. Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans. Infection And Immunity 2001, 69: 4958-4968. PMID: 11447174, PMCID: PMC98588, DOI: 10.1128/iai.69.8.4958-4968.2001.Peer-Reviewed Original ResearchConceptsHumoral immune responseImmune responseNatural infectionLeptospiral proteinsInfection-associated antigenConvalescent-phase seraPositive reactionConvalescent phaseHumoral responseHealthy individualsHuman leptospirosisControl individualsVaccine designImmunodominant antigensQuantitative immunoblot analysisTwo-dimensional immunoblotsProtein antigensPathogenic leptospiral strainsGenus LeptospiraInfectionAntigenLeptospirosisSerumImmunodominant proteinsPatients