2023
Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi
Andrade A, Santos N, Bastos A, Pontual J, Araújo C, Lima A, Martinez L, Ferreira A, Aguiar A, Teles C, Guido R, Santana R, Lopes S, Medeiros J, Rizopoulos Z, Vinetz J, Campo B, Lacerda M, Araújo M. Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi. Scientific Reports 2023, 13: 18207. PMID: 37875508, PMCID: PMC10598059, DOI: 10.1038/s41598-023-44556-y.Peer-Reviewed Original ResearchConceptsP. vivax liver stagesPlasmodium vivax infectionPlasmodium vivax sporozoitesLiver stage parasitesP. vivax sporozoitesFemale anopheline mosquitoesVivax infectionLiver stagesHepatocyte infectionVaccine developmentStage parasitesSporozoite viabilityMembrane feeding systemSporozoite productionDrug developmentSporozoitesAnopheline mosquitoesInfectionParasite biologyArtificial membrane feeding systemPatientsSimple centrifugation methodSerum replacementBlood
2018
Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon
Moreno M, Tong-Rios C, Orjuela-Sanchez P, Carrasco-Escobar G, Campo B, Gamboa D, Winzeler EA, Vinetz JM. Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon. ACS Infectious Diseases 2018, 4: 541-548. PMID: 29465219, PMCID: PMC5902790, DOI: 10.1021/acsinfecdis.7b00195.Peer-Reviewed Original ResearchConceptsPlasmodium vivax sporozoitesDonor seraPlasmodium vivax liver stagesStandard membrane feeding assaysMalaria-endemic settingsMembrane feeding assaysSporozoite productionInfectious blood mealInfected subjectsNaïve serumBlood-fed mosquitoesLiver stagesDonor bloodBlood samplesVaccine developmentMosquito midgutSerumBlood mealBloodMosquito survivalSporozoitesMosquitoesAnophelesAnticoagulantsInfection