Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion
Chaurasia R, Liang C, How K, Vieira D, Vinetz J. Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion. The Protein Journal 2023, 42: 792-801. PMID: 37653175, DOI: 10.1007/s10930-023-10152-2.Peer-Reviewed Original ResearchMCherry fusion proteinsFusion proteinMCherry tagGene familyMCherry fusionsProtein productionFluorescent fusion proteinsRecombinant protein expressionRecombinant protein productionVM proteinsSuch proteinsFunctional proteinsCell biologyLike domainFast protein liquid chromatographyLeptospiral virulenceSoluble proteinUnique memberPink coloniesProtein scienceStructural predictionsProtein liquid chromatographyRicin BFunctional studiesProteinIn silico prediction of molecular mechanisms of toxicity mediated by the leptospiral PF07598 gene family-encoded virulence-modifying proteins
Chaurasia R, Vinetz J. In silico prediction of molecular mechanisms of toxicity mediated by the leptospiral PF07598 gene family-encoded virulence-modifying proteins. Frontiers In Molecular Biosciences 2023, 9: 1092197. PMID: 36756251, PMCID: PMC9900628, DOI: 10.3389/fmolb.2022.1092197.Peer-Reviewed Original ResearchMolecular dynamics simulationsProtein structure-function relationshipsSecondary structure contentDynamics simulationsVM proteinsStructure-function relationshipsStructure-function studiesStructure contentDivalent cationsSurface bindingRicin BMechanistic understandingBinding residuesC-terminal similaritySilico predictionCationsIonsRicin toxinLeptospirosis pathogenesisDNase I.AB toxins