2023
Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion
Chaurasia R, Liang C, How K, Vieira D, Vinetz J. Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion. The Protein Journal 2023, 42: 792-801. PMID: 37653175, DOI: 10.1007/s10930-023-10152-2.Peer-Reviewed Original ResearchMCherry fusion proteinsFusion proteinMCherry tagGene familyMCherry fusionsProtein productionFluorescent fusion proteinsRecombinant protein expressionRecombinant protein productionVM proteinsSuch proteinsFunctional proteinsCell biologyLike domainFast protein liquid chromatographyLeptospiral virulenceSoluble proteinUnique memberPink coloniesProtein scienceStructural predictionsProtein liquid chromatographyRicin BFunctional studiesProtein
2022
Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1
Kaur H, Garber L, Murphy JW, Vinetz JM. Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1. Protein Science 2022, 31: e4289. PMID: 35481637, PMCID: PMC8994504, DOI: 10.1002/pro.4289.Peer-Reviewed Original ResearchConceptsPutative chitin-binding domainChitin-binding domainPlasmodium ookinetesProtein-protein interactionsRecombinant protein productionIntermolecular protein-protein interactionsMosquito midgut invasionStructure-function analysisEnzymatic activityIntramolecular disulfide linkagesIntermolecular disulfide bondingChitin-containing peritrophic matrixParasite cladesMidgut invasionPeritrophic matrixCysteine residuesProtein productionChitinase activityBiochemical studiesDisulfide bondingUnpaired cysteineChitinaseMalaria parasitesE. coliMutants