2022
Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1
Kaur H, Garber L, Murphy JW, Vinetz JM. Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1. Protein Science 2022, 31: e4289. PMID: 35481637, PMCID: PMC8994504, DOI: 10.1002/pro.4289.Peer-Reviewed Original ResearchConceptsPutative chitin-binding domainChitin-binding domainPlasmodium ookinetesProtein-protein interactionsRecombinant protein productionIntermolecular protein-protein interactionsMosquito midgut invasionStructure-function analysisEnzymatic activityIntramolecular disulfide linkagesIntermolecular disulfide bondingChitin-containing peritrophic matrixParasite cladesMidgut invasionPeritrophic matrixCysteine residuesProtein productionChitinase activityBiochemical studiesDisulfide bondingUnpaired cysteineChitinaseMalaria parasitesE. coliMutants
2001
Knockout of the Rodent Malaria Parasite Chitinase PbCHT1 Reduces Infectivity to Mosquitoes
Dessens J, Mendoza J, Claudianos C, Vinetz J, Khater E, Hassard S, Ranawaka G, Sinden R. Knockout of the Rodent Malaria Parasite Chitinase PbCHT1 Reduces Infectivity to Mosquitoes. Infection And Immunity 2001, 69: 4041-4047. PMID: 11349074, PMCID: PMC98467, DOI: 10.1128/iai.69.6.4041-4047.2001.Peer-Reviewed Original ResearchConceptsPeritrophic matrixChitinase geneChitinase activityHuman malaria parasite Plasmodium falciparumMalaria parasite Plasmodium falciparumPlasmodium-mosquito interactionsAvian malaria parasite Plasmodium gallinaceumAvian malaria transmissionParasite Plasmodium falciparumMalaria parasite Plasmodium gallinaceumChitin-containing structuresNull mutantsRodent malaria parasiteInfected blood mealMalaria ookinetesTransmission-blocking drugsAnopheles stephensi mosquitoesMidgut invasionStructural orthologsMosquito midgutOokinete invasionPM disruptionMidgut epitheliumTargeted disruptionMalaria transmission