2014
Stratified control of IGF-I expression by hypoxia and stress hormones in osteoblasts
McCarthy TL, Yun Z, Madri JA, Centrella M. Stratified control of IGF-I expression by hypoxia and stress hormones in osteoblasts. Gene 2014, 539: 141-151. PMID: 24440782, PMCID: PMC4316208, DOI: 10.1016/j.gene.2014.01.011.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsCCAAT-Enhancer-Binding Protein-deltaCell HypoxiaCells, CulturedCore Binding Factor Alpha 1 SubunitDinoprostoneDNADNA ReplicationGene Expression RegulationHydrocortisoneInsulin-Like Growth Factor IOsteoblastsOxygenPromoter Regions, GeneticProtein BiosynthesisRatsRats, Sprague-DawleyRegulatory Elements, TranscriptionalRNA, MessengerConceptsIGF-I expressionStress hormonesIGF-I gene promoterIGF-I mRNAInfluence of PGE2Transcription factor C/EBPδOsteoblast transcription factor Runx2Hypoxic inhibitionI expressionPGE2Stimulatory effectGlucocorticoidsHypoxiaHIF activityHypoxic stressTranscription factor Runx2Bone cellsIGFProlonged exposureHormoneAlkaline phosphatase activitySystemic regulationOsteoblastsResponse elementRunx2
2007
Loss of MMP-2 disrupts skeletal and craniofacial development and results in decreased bone mineralization, joint erosion and defects in osteoblast and osteoclast growth
Mosig RA, Dowling O, DiFeo A, Ramirez MC, Parker IC, Abe E, Diouri J, Al Aqeel AA, Wylie JD, Oblander SA, Madri J, Bianco P, Apte SS, Zaidi M, Doty SB, Majeska RJ, Schaffler MB, Martignetti JA. Loss of MMP-2 disrupts skeletal and craniofacial development and results in decreased bone mineralization, joint erosion and defects in osteoblast and osteoclast growth. Human Molecular Genetics 2007, 16: 1113-1123. PMID: 17400654, PMCID: PMC2576517, DOI: 10.1093/hmg/ddm060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArthritisBone and BonesBone RemodelingCalcification, PhysiologicCell ProliferationCells, CulturedCraniofacial AbnormalitiesGene DeletionHumansImmunohistochemistryJointsMatrix Metalloproteinase 2MiceMice, KnockoutOsteoblastsOsteoclastsReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingTime FactorsTomography, X-Ray ComputedConceptsMMP2-/- miceMMP-2Arthritis syndromeArticular cartilage destructionOsteoclast growthBone mineral densityDays of lifeWeeks of lifeWeeks of ageMMP-2 overexpressionJoint erosionsBone lossCartilage destructionNormal cell numbersPathophysiological mechanismsOsteoclast numberVivo physiological roleMineral densityControl littermatesAnatomical distributionBone disordersMurine modelMineralization defectMulticentric osteolysisDisease pathogenesis