2020
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells.
Boisvert EM, Means RE, Michaud M, Thomson JJ, Madri JA, Katz SG. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 PMID: 32202516, PMCID: PMC7245934, DOI: 10.3791/60379.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsCell typesStem cellsIntrinsic developmental cuesHuman embryonic stem cellsHuman pluripotent stem cellsBrain organoidsBrain cell typesPluripotent stem cellsBasement membrane matrixMultiple cell typesDevelopmental cuesUse of organoidsExogenous growth factorsQuantitative reverse transcription polymerase chain reactionMultitude of diseasesHuman brain organoidsOrganoid growthSingle cellsReal-time quantitative reverse transcription polymerase chain reactionSpatial organizationOrganoidsGenetic disordersGrowth factorReverse transcription-polymerase chain reaction
2019
Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids
Boisvert EM, Means RE, Michaud M, Madri JA, Katz SG. Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids. Cell Death & Disease 2019, 10: 325. PMID: 30975982, PMCID: PMC6459920, DOI: 10.1038/s41419-019-1553-x.Peer-Reviewed Original ResearchConceptsNeonatal hypoxic injuryBrain developmentEfficacy of minocyclineLow birth weightUse of minocyclineEffects of hypoxiaNormal brain developmentCerebral organoid modelHuman brain organoidsLater time pointsAnimal model systemsNeonatal hypoxicDevastating causeCerebral palsySignificant morbidityHuman brain developmentNeurological consequencesBirth weightHypoxic injuryNeuronal deathCortical neuronsInjury resultsGlial cellsForebrain markersPotential treatment
2009
Strain Differences in Behavioral and Cellular Responses to Perinatal Hypoxia and Relationships to Neural Stem Cell Survival and Self-Renewal Modeling the Neurovascular Niche
Li Q, Liu J, Michaud M, Schwartz ML, Madri JA. Strain Differences in Behavioral and Cellular Responses to Perinatal Hypoxia and Relationships to Neural Stem Cell Survival and Self-Renewal Modeling the Neurovascular Niche. American Journal Of Pathology 2009, 175: 2133-2145. PMID: 19815710, PMCID: PMC2774076, DOI: 10.2353/ajpath.2009.090354.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBehavior, AnimalCell DifferentiationCell MovementCell SurvivalCells, CulturedChemokine CXCL12Endothelial CellsEnzyme ActivationFemaleHumansHypoxiaHypoxia-Inducible Factor 1, alpha SubunitHypoxia-Inducible Factor-Proline DioxygenasesInfantInfant, NewbornInfant, PrematureMaleMiceMice, Inbred C57BLMice, Inbred StrainsNeuronsNeuropsychological TestsPhosphatidylinositol 3-KinasesProcollagen-Proline DioxygenaseProto-Oncogene Proteins c-aktSignal TransductionStem CellsConceptsChronic hypoxiaC57 miceHIF-1alphaLow birth weight infant populationMatrix metalloproteinase-9 activityStromal-derived factor-1CD-1 miceMetalloproteinase-9 activityAdult C57 miceHypoxia-induced factorNeural stem cell survivalHigher apoptosis ratePerinatal hypoxiaRepair/recoveryClinical improvementNeurodevelopmental handicapPreventive therapyPremature infantsNeurogenic zonesNeurovascular nicheInfant populationC57BL/6 pupsProlyl hydroxylase domain 2Migratory responsivenessStem cell survival
2008
Co-culture of primary neural progenitor and endothelial cells in a macroporous gel promotes stable vascular networks in vivo
Rauch MF, Michaud M, Xu H, Madri JA, Lavik EB. Co-culture of primary neural progenitor and endothelial cells in a macroporous gel promotes stable vascular networks in vivo. Journal Of Biomaterials Science Polymer Edition 2008, 19: 1469-1485. PMID: 18973724, DOI: 10.1163/156856208786140409.Peer-Reviewed Original Research
2006
Modeling the neurovascular niche: VEGF‐ and BDNF‐mediated cross‐talk between neural stem cells and endothelial cells: An in vitro study
Li Q, Ford MC, Lavik EB, Madri JA. Modeling the neurovascular niche: VEGF‐ and BDNF‐mediated cross‐talk between neural stem cells and endothelial cells: An in vitro study. Journal Of Neuroscience Research 2006, 84: 1656-1668. PMID: 17061253, DOI: 10.1002/jnr.21087.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAnimals, NewbornBrainBrain-Derived Neurotrophic FactorCell CommunicationCell ProliferationCells, CulturedCoculture TechniquesEndothelial CellsEnzyme-Linked Immunosorbent AssayGreen Fluorescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, Electron, TransmissionModels, BiologicalNerve Tissue ProteinsNeuronsNitric OxidePlatelet Endothelial Cell Adhesion Molecule-1Stem CellsVascular Endothelial Growth Factor AConceptsBrain-derived neurotrophic factorBrain-derived endothelial cellsNeural stem cellsNeurovascular nicheTube formationResident neural stem cellsEndothelial cellsCell-derived soluble factorsVascular endothelial growth factorStem cellsNitric oxide scavengerEndothelial growth factorPaucity of dataExogenous NO donorNeurotrophic factorStem cell modulationVascular tube formationCell modulationENOS activationNO donorSoluble factorsGrowth factorNeuronal differentiationReciprocal modulationInductionA macroporous hydrogel for the coculture of neural progenitor and endothelial cells to form functional vascular networks in vivo
Ford MC, Bertram JP, Hynes SR, Michaud M, Li Q, Young M, Segal SS, Madri JA, Lavik EB. A macroporous hydrogel for the coculture of neural progenitor and endothelial cells to form functional vascular networks in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 2512-2517. PMID: 16473951, PMCID: PMC1413771, DOI: 10.1073/pnas.0506020102.Peer-Reviewed Original Research
2002
Paracrine and Autocrine Functions of Neuronal Vascular Endothelial Growth Factor (VEGF) in the Central Nervous System*
Ogunshola OO, Antic A, Donoghue MJ, Fan SY, Kim H, Stewart WB, Madri JA, Ment LR. Paracrine and Autocrine Functions of Neuronal Vascular Endothelial Growth Factor (VEGF) in the Central Nervous System*. Journal Of Biological Chemistry 2002, 277: 11410-11415. PMID: 11777931, DOI: 10.1074/jbc.m111085200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCerebral CortexEndothelial Growth FactorsImmunohistochemistryLymphokinesMAP Kinase Kinase Kinase 1MiceNeuronsPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Serine-Threonine KinasesRatsReceptor Protein-Tyrosine KinasesReceptors, Growth FactorReceptors, Vascular Endothelial Growth FactorSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsVascular endothelial growth factorNeuronal vascular endothelial growth factorExtracellular signal-regulated protein kinaseSignal-regulated protein kinaseCentral nervous systemFlk-1Inhibition of phosphatidylinositolPost-mitotic neuronsTyrosine phosphorylation levelsInhibition of MEKEndothelial growth factorAutocrine functionGrowth factorEmbryonic mouse forebrainNervous systemMaintenance of neuronsProtein kinaseTyrosine phosphorylationNovel functionNeuronal culturesPhosphorylation levelsSpecific inhibitorExpression of VEGFExogenous additionEmbryonic cortical neurons
2000
Neuronal VEGF expression correlates with angiogenesis in postnatal developing rat brain
Ogunshola O, Stewart W, Mihalcik V, Solli T, Madri J, Ment L. Neuronal VEGF expression correlates with angiogenesis in postnatal developing rat brain. Brain Research 2000, 119: 139-153. PMID: 10648880, DOI: 10.1016/s0165-3806(99)00125-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell CountCerebral CortexChronic DiseaseEndothelial Growth FactorsGene Expression Regulation, DevelopmentalGlial Fibrillary Acidic ProteinHypoxia, BrainLymphokinesMicrocirculationNeovascularization, PhysiologicNeurogliaNeuronsPlatelet Endothelial Cell Adhesion Molecule-1RatsRNA, MessengerVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsVascular endothelial growth factorLocalization of VEGFCortical neuronsGlial cellsNeuronal expressionChronic sublethal hypoxiaRat brain cortexAge-matched controlsCortical brain tissueHypoxia-driven angiogenesisPostnatal day 3Endothelial growth factorDensity of vesselsWestern blot analysisHypoxic animalsHypoxic chamberVascular bedNewborn ratsRat brainBrain cortexControl animalsDay 3High neuronal expressionSublethal hypoxiaPostnatal development