2017
As human lung microvascular endothelia achieve confluence, src family kinases are activated, and tyrosine-phosphorylated p120 catenin physically couples NEU1 sialidase to CD31
Hyun SW, Liu A, Liu Z, Lillehoj EP, Madri JA, Reynolds AB, Goldblum SE. As human lung microvascular endothelia achieve confluence, src family kinases are activated, and tyrosine-phosphorylated p120 catenin physically couples NEU1 sialidase to CD31. Cellular Signalling 2017, 35: 1-15. PMID: 28343945, DOI: 10.1016/j.cellsig.2017.03.014.Peer-Reviewed Original ResearchMeSH KeywordsCateninsCell LineCell-Free SystemDelta CateninEndothelial CellsHumansLungMicrovesselsN-Acetylneuraminic AcidNeovascularization, PhysiologicNeuraminidasePhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein BindingProtein Interaction MapsProto-Oncogene Proteins c-fynProto-Oncogene Proteins c-yesSignal TransductionSrc-Family Kinases
2016
Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices
Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomaterialia 2016, 46: 91-100. PMID: 27693690, PMCID: PMC5451113, DOI: 10.1016/j.actbio.2016.09.043.Peer-Reviewed Original ResearchConceptsExtracellular matrixLung extracellular matrixMatrix proteinsECM-associated proteinsCell-matrix interactionsProtein extraction methodsWhole organ regenerationRegenerative medicineOrganotypic cell culturesQuantitative proteomicsAcellular extracellular matrixECM proteinsOrgan regenerationCell adhesionProteomicsProtein analysisQuantitative biochemical dataProteinPotent substrateXenogeneic extracellular matrixTargeted proteomicsCell nuclear antigenBiochemical dataImportant glycoproteinCell cultures
2014
NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia*
Lee C, Liu A, Miranda-Ribera A, Hyun SW, Lillehoj EP, Cross AS, Passaniti A, Grimm PR, Kim BY, Welling PA, Madri JA, DeLisser HM, Goldblum SE. NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia*. Journal Of Biological Chemistry 2014, 289: 9121-9135. PMID: 24550400, PMCID: PMC3979388, DOI: 10.1074/jbc.m114.555888.Peer-Reviewed Original ResearchConceptsHuman pulmonary microvascular ECsCapillary-like tube formationEC tube formationTube formationCell adhesion molecule-1Pulmonary microvascular ECsHuman Lung Microvascular EndotheliaNeu1 sialidaseLung microvascular endotheliumAdhesion molecule-1Endothelial cell adhesion molecule-1Platelet endothelial cell adhesion molecule-1Endothelial cell expressionMultiplicity of infectionMicrovascular endotheliumMolecule-1Microvascular ECsCell expressionCD31Matrigel substrateSialylation statePeanut agglutinin lectinAdhesion moleculesInhibitory effectAngiogenic phenotype
2003
PECAM-1 promotes β-catenin accumulation and stimulates endothelial cell proliferation
Biswas P, Canosa S, Schoenfeld J, Schoenfeld D, Tucker A, Madri JA. PECAM-1 promotes β-catenin accumulation and stimulates endothelial cell proliferation. Biochemical And Biophysical Research Communications 2003, 303: 212-218. PMID: 12646189, DOI: 10.1016/s0006-291x(03)00313-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBeta CateninBlotting, WesternCell AdhesionCell DivisionCytoplasmCytoskeletal ProteinsEndotheliumFlow CytometryHumansLungMiceMice, KnockoutMicroscopy, FluorescencePlatelet Endothelial Cell Adhesion Molecule-1Precipitin TestsSignal TransductionTrans-ActivatorsTranscription, GeneticTransfectionConceptsPECAM-1-positive endothelial cellsBeta-catenin proteinCell proliferationEndothelial cellsPECAM-1Beta-catenin localizationCytoplasmic domainΒ-catenin accumulationFull-length PECAM-1Functional consequencesEndothelial cell proliferationCell membraneKnockout animalsAdhesion moleculesLess accumulationCellsAccumulationProliferative rateProliferationMembraneProteinBinds
1984
An acellular human amnionic membrane model for in vitro culture of type ii pneumocytes: The role of the basement membrane in cell morphology and function
Lwebuga‐Mukasa J, Thulin G, Madri J, Barrett C, Warshaw J. An acellular human amnionic membrane model for in vitro culture of type ii pneumocytes: The role of the basement membrane in cell morphology and function. Journal Of Cellular Physiology 1984, 121: 215-225. PMID: 6548224, DOI: 10.1002/jcp.1041210127.Peer-Reviewed Original Research
1983
Ultrastructural morphology and domain structure of a unique collagenous component of basement membranes.
Madri J, Foellmer H, Furthmayr H. Ultrastructural morphology and domain structure of a unique collagenous component of basement membranes. Biochemistry 1983, 22: 2797-804. PMID: 6409144, DOI: 10.1021/bi00281a005.Peer-Reviewed Original Research
1980
Collagen polymorphism in the lung An immunochemical study of pulmonary fibrosis
Madri J, Furthmayr H. Collagen polymorphism in the lung An immunochemical study of pulmonary fibrosis. Human Pathology 1980, 11: 353-366. PMID: 6997183, DOI: 10.1016/s0046-8177(80)80031-1.Peer-Reviewed Original ResearchConceptsPerivascular localizationLung samplesFibrotic human lungSmooth muscle cell proliferationType IIndirect immunofluorescence techniqueLung type IMuscle cell proliferationType VType IIICapillary basement membranePulmonary fibrosisFibrotic processAlveolar septaType IV collagenImmunofluorescence techniqueHuman lungCell proliferationInterstitiumMarked increaseType IVBasement membraneImmunochemical studiesIrregular localizationIV collagen
1979
Isolation and tissue localization of type AB2 collagen from normal lung parenchyma.
Madri J, Furthmayr H. Isolation and tissue localization of type AB2 collagen from normal lung parenchyma. American Journal Of Pathology 1979, 94: 323-31. PMID: 371411, PMCID: PMC2042247.Peer-Reviewed Original ResearchConceptsNormal lung parenchymaLung parenchymaAffinity-purified rabbit antibodiesLung tissuePassive hemagglutinationPlacental membranesChorionic membraneAB2 collagenTissue localizationIndirect immunofluorescence microscopyRabbit antibodiesBasement membraneChain ratioParenchymaImmunofluorescence microscopyCollagenPolyacrylamide gel electrophoresisGel electrophoresisAlpha ADifferential salt extractionCell surface