2016
Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices
Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomaterialia 2016, 46: 91-100. PMID: 27693690, PMCID: PMC5451113, DOI: 10.1016/j.actbio.2016.09.043.Peer-Reviewed Original ResearchConceptsExtracellular matrixLung extracellular matrixMatrix proteinsECM-associated proteinsCell-matrix interactionsProtein extraction methodsWhole organ regenerationRegenerative medicineOrganotypic cell culturesQuantitative proteomicsAcellular extracellular matrixECM proteinsOrgan regenerationCell adhesionProteomicsProtein analysisQuantitative biochemical dataProteinPotent substrateXenogeneic extracellular matrixTargeted proteomicsCell nuclear antigenBiochemical dataImportant glycoproteinCell cultures
2003
PECAM-1: old friend, new partners
Ilan N, Madri JA. PECAM-1: old friend, new partners. Current Opinion In Cell Biology 2003, 15: 515-524. PMID: 14519385, DOI: 10.1016/s0955-0674(03)00100-5.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAlpha CateninAnimalsApoptosisBeta CateninCapillary PermeabilityCell Adhesion MoleculesCytoskeletal ProteinsDNA-Binding ProteinsHumansIntermediate FilamentsMilk ProteinsModels, MolecularPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein Structure, TertiarySignal TransductionSTAT5 Transcription FactorTrans-ActivatorsVascular Endothelial Growth Factor A
2000
Platelet-Endothelial Cell Adhesion Molecule-1 (CD31), a Scaffolding Molecule for Selected Catenin Family Members Whose Binding Is Mediated by Different Tyrosine and Serine/Threonine Phosphorylation*
Ilan N, Cheung L, Pinter E, Madri J. Platelet-Endothelial Cell Adhesion Molecule-1 (CD31), a Scaffolding Molecule for Selected Catenin Family Members Whose Binding Is Mediated by Different Tyrosine and Serine/Threonine Phosphorylation*. Journal Of Biological Chemistry 2000, 275: 21435-21443. PMID: 10801826, DOI: 10.1074/jbc.m001857200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBinding SitesCadherinsCell Adhesion MoleculesCell LineCells, CulturedCytoskeletal ProteinsCytoskeletonDesmoplakinsEmbryo, MammalianEndothelium, VascularGamma CateninHumansMicePhosphorylationPhosphoserinePhosphothreoninePhosphotyrosinePlatelet Endothelial Cell Adhesion Molecule-1Protein Kinase CRecombinant Fusion ProteinsSignal TransductionTrans-ActivatorsUmbilical VeinsYolk SacConceptsSerine/threonine phosphorylationThreonine phosphorylationCell-cell junctionsSpecific tyrosine residuesSignal transduction pathwaysPECAM-1 functionsTyrosine phosphorylation levelsInsoluble cytoskeletal fractionPECAM-1Beta-catenin localizationCytoskeleton interactionsPKC enzymeTransduction pathwaysCell adhesion moleculeCytoskeletal fractionTyrosine residuesMolecular mechanismsDifferent tyrosinePlatelet endothelial cell adhesion molecule-1Phosphorylation levelsITAM domainSW480 cellsEndothelium-specific markersPhosphorylationPlatelet endothelial cell adhesion moleculeCell Migration in the Immune System: the Evolving Inter‐Related Roles of Adhesion Molecules and Proteinases
Madri J, Graesser D. Cell Migration in the Immune System: the Evolving Inter‐Related Roles of Adhesion Molecules and Proteinases. Journal Of Immunology Research 2000, 7: 103-116. PMID: 11097205, PMCID: PMC2276058, DOI: 10.1155/2000/79045.Peer-Reviewed Original Research
1996
The roles of adhesion molecules and proteinases in lymphocyte transendothelial migration
Madri J, Graesser D, Haas T. The roles of adhesion molecules and proteinases in lymphocyte transendothelial migration. Biochemistry And Cell Biology 1996, 74: 749-757. PMID: 9164645, DOI: 10.1139/o96-082.Peer-Reviewed Original ResearchIntegrin engagement mediates tyrosine dephosphorylation on platelet-endothelial cell adhesion molecule 1.
Lu T, Yan L, Madri J. Integrin engagement mediates tyrosine dephosphorylation on platelet-endothelial cell adhesion molecule 1. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 11808-11813. PMID: 8876219, PMCID: PMC38140, DOI: 10.1073/pnas.93.21.11808.Peer-Reviewed Original Research3T3 CellsAnimalsCell AdhesionCell Adhesion MoleculesCell MovementCells, CulturedEndothelium, VascularFibronectinsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesHumansMaleMiceMutagenesis, Site-DirectedPhosphorylationPhosphotyrosinePlatelet Endothelial Cell Adhesion Molecule-1Protein Tyrosine PhosphatasesProtein-Tyrosine KinasesRatsRecombinant ProteinsTransfectionTyrosineUmbilical Veins
1995
Modulation of cell spreading and migration by pp125FAK phosphorylation.
Sankar S, Mahooti-Brooks N, Hu G, Madri J. Modulation of cell spreading and migration by pp125FAK phosphorylation. American Journal Of Pathology 1995, 147: 601-8. PMID: 7677174, PMCID: PMC1870973.Peer-Reviewed Original ResearchConceptsPp125FAK tyrosine phosphorylationTyrosine phosphorylationCell migrationCell spreadingIntegrin-mediated signalingFocal adhesion kinaseKinase-negative mutantPp125FAK phosphorylationAdhesion kinaseMigration ratePhosphorylationCascade pathwayFaster migration rateControl cellsSlower migration rateType I collagenEnhanced levelsCellsI collagenFibronectinMigrationPp125FAKMutantsKinaseSignalingHuman capillary endothelial cells from abdominal wall adipose tissue: Isolation using an anti-pecam antibody
Springhorn J, Madri J, Squinto S. Human capillary endothelial cells from abdominal wall adipose tissue: Isolation using an anti-pecam antibody. In Vitro Cellular & Developmental Biology - Animal 1995, 31: 473-481. PMID: 8589892, DOI: 10.1007/bf02634261.Peer-Reviewed Original ResearchMeSH KeywordsAbdomenAdipose TissueAnimalsAntibodies, MonoclonalAntigens, Differentiation, MyelomonocyticCell Adhesion MoleculesCell SeparationCell Transformation, ViralCells, CulturedEndothelium, VascularGrowth HormoneHumansLectinsPlant LectinsPlatelet Endothelial Cell Adhesion Molecule-1Vascular Cell Adhesion Molecule-1Von Willebrand FactorConceptsCapillary endothelial cellsHuman capillary endothelial cellsAnti-PECAM antibodyEndothelial cellsCellular adhesion moleculesVon Willebrand factorAdipose tissueDensity lipoproteinAdhesion molecule antibodyGrowth hormoneFlow cytometryMolecule antibodiesAdhesion moleculesAntibodiesWillebrand factorPassage sixNormal patternAntibody methodCell isolatesImmunofluorescent labelingAgglutinin lectinType I
1994
The induction of 72-kD gelatinase in T cells upon adhesion to endothelial cells is VCAM-1 dependent.
Romanic A, Madri J. The induction of 72-kD gelatinase in T cells upon adhesion to endothelial cells is VCAM-1 dependent. Journal Of Cell Biology 1994, 125: 1165-1178. PMID: 7515069, PMCID: PMC2120055, DOI: 10.1083/jcb.125.5.1165.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Recombinant VCAM-1T cellsT cell transmigrationEndothelial cellsNegative endothelial cellsCell transmigrationCell adhesion molecule-1Transmigrated T cellsExperimental autoimmune encephalomyelitisT cell extravasationAdhesion molecule-1Late antigen-4VCAM-1 positive cellsMyelin basic proteinAutoimmune encephalomyelitisEndothelial cell monolayersAntigen-4Brain parenchymaNormal miceEndothelial cell layerPositive cellsMolecule-1Perivascular tissueT cell adhesion
1993
Differential induction of VCAM-1 on human iliac venous and arterial endothelial cells and its role in adhesion.
Hauser I, Johnson D, Madri J. Differential induction of VCAM-1 on human iliac venous and arterial endothelial cells and its role in adhesion. The Journal Of Immunology 1993, 151: 5172-85. PMID: 7693806, DOI: 10.4049/jimmunol.151.10.5172.Peer-Reviewed Original ResearchMeSH KeywordsAdultCell AdhesionCell Adhesion MoleculesCells, CulturedEndothelium, VascularE-SelectinFemaleHumansIliac ArteryIliac VeinIntercellular Adhesion Molecule-1Lymphocyte Function-Associated Antigen-1Middle AgedReceptors, Very Late AntigenRNA, MessengerTetradecanoylphorbol AcetateTumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1ConceptsArterial endothelial cellsICAM-1VCAM-1Venous endothelial cellsEndothelial cellsTNF-alphaU937 cell adhesionELAM-1VCAM-1 cell surface expressionTNF-alpha-induced VCAM-1 expressionAdhesion molecules VCAM-1ICAM-2U937 cellsAdhesion molecules ICAM-1TNF-alpha-induced expressionLate activation AgVCAM-1 expressionMolecules ICAM-1VCAM-1/VLAMonocyte-like cell line U937TNF-alpha treatmentVessel endothelial cellsAdhesion of PMALarge vessel endothelial cellsEC typesSurface expression of alpha 4 integrin by CD4 T cells is required for their entry into brain parenchyma.
Baron JL, Madri JA, Ruddle NH, Hashim G, Janeway CA. Surface expression of alpha 4 integrin by CD4 T cells is required for their entry into brain parenchyma. Journal Of Experimental Medicine 1993, 177: 57-68. PMID: 7678116, PMCID: PMC2190872, DOI: 10.1084/jem.177.1.57.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalBrainCD4-Positive T-LymphocytesCell Adhesion MoleculesCell MovementClone CellsEncephalomyelitis, Autoimmune, ExperimentalIntercellular Adhesion Molecule-1MiceMice, Inbred BALB CMyelin Basic ProteinReceptors, Very Late AntigenVascular Cell Adhesion Molecule-1ConceptsAlpha 4 integrinsExperimental autoimmune encephalomyelitisAdhesion molecule-1T cellsBrain parenchymaT cell linesEncephalitogenic clonesAlpha 4Surface expressionMolecule-1Recombinant vascular cell adhesion molecule-1T helper type 1 clonesCD4 T cell linesCloned T cell linesTh1 cytokine interleukin-2Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Tumor necrosis factor betaTh1 T cellsEffector T cellsCD4 T cellsMajor pathogenic factorT cell entryNecrosis factor beta
1992
Platelet endothelial cell adhesion molecule, PECAM‐1, modulates cell migration
Schimmenti L, Yan H, Madri J, Albelda S. Platelet endothelial cell adhesion molecule, PECAM‐1, modulates cell migration. Journal Of Cellular Physiology 1992, 153: 417-428. PMID: 1429859, DOI: 10.1002/jcp.1041530222.Peer-Reviewed Original ResearchConceptsCell-cell adhesionCell-cell adhesion moleculesCell-cell contactPlatelet endothelial cell adhesion molecule-1Cell migrationIntegral membrane proteinsAdhesion moleculesPECAM-1 moleculesControl cellsCell surface adhesion moleculesModulates cell migrationNeomycin resistance geneEndothelial cell migrationMembrane proteinsCell adhesion moleculeEndothelial cellsExtracellular domainSurface adhesion moleculesNull cellsNIH/3T3 cellsResistance genesExtracellular matrixTransfectantsAdjacent cellsMigration assaysCancer cell binding to E-selectin transfected human endothelia
Merwin J, Madri J, Lynch M. Cancer cell binding to E-selectin transfected human endothelia. Biochemical And Biophysical Research Communications 1992, 189: 315-323. PMID: 1280420, DOI: 10.1016/0006-291x(92)91560-d.Peer-Reviewed Original Research