2024
Multi-omics profiling of DNA methylation and gene expression alterations in human cocaine use disorder
Zillich E, Belschner H, Avetyan D, Andrade-Brito D, Martínez-Magaña J, Frank J, Mechawar N, Turecki G, Cabana-Domínguez J, Fernàndez-Castillo N, Cormand B, Montalvo-Ortiz J, Nöthen M, Hansson A, Rietschel M, Spanagel R, Witt S, Zillich L. Multi-omics profiling of DNA methylation and gene expression alterations in human cocaine use disorder. Translational Psychiatry 2024, 14: 428. PMID: 39384764, PMCID: PMC11464785, DOI: 10.1038/s41398-024-03139-9.Peer-Reviewed Original ResearchConceptsCocaine use disorderUse disorderAlternative splicingHuman prefrontal cortexProfiling of DNA methylationBrodmann area 9Differential alternative splicingDeregulated biological processesPostmortem brain tissueMulti-omics approachCocaine intakeMulti-omics studiesPrefrontal cortexBrain alterationsMulti-omics profilingGene expression alterationsArea 9Fatty acid metabolismReceptor-targeting drugsSpliced transcriptsEpigenome-wideDNA methylationNeuronal morphogenesisAS changesDrug repositioning analysisW69. ALTERNATIVE SPLICING DYNAMICS IN HUMAN PREFRONTAL CORTEX SUBREGIONS
Nunez D, Martinez-Magana J, Nagamatsu S, Andrade-Brito D, Hernandez-Amaya L, Montalvo-Ortiz J. W69. ALTERNATIVE SPLICING DYNAMICS IN HUMAN PREFRONTAL CORTEX SUBREGIONS. European Neuropsychopharmacology 2024, 87: 138-139. DOI: 10.1016/j.euroneuro.2024.08.278.Peer-Reviewed Original ResearchDorsal anterior cingulate cortexPrefrontal cortex subregionsPrefrontal cortexOrbitofrontal cortexDifferential transcript usagePercent-spliced-inSubgenual prefrontal cortexPrefrontal cortex regionsAnterior cingulate cortexDorsolateral prefrontal cortexDSM-IV criteriaAS eventsTranscript usageSynaptic processesExon skippingDSM-IVSCID-1Cingulate cortexIntron retentionMood disordersPsychiatric disordersSplice siteAlternative splicingCognitive functionNon-psychiatric diagnosesF91. MAPPING THE EFFECTS OF OPIOID USE DISORDER GENETIC ASSOCIATED VARIANTS IN BRAIN PATHWAYS AT A SINGLE CELL LEVEL
Rivera-Hernández M, Martínez-Magaña J, Brennand K, Montalvo-Ortiz J. F91. MAPPING THE EFFECTS OF OPIOID USE DISORDER GENETIC ASSOCIATED VARIANTS IN BRAIN PATHWAYS AT A SINGLE CELL LEVEL. European Neuropsychopharmacology 2024, 87: 254. DOI: 10.1016/j.euroneuro.2024.08.502.Peer-Reviewed Original ResearchOpioid use disorderDopaminergic neuronsReward-related learningOrbital frontal cortexGenetic variantsFunction of dopaminergic neuronsMap genetic variantsGenome-wide studiesCell projection organizationSingle-cell expression profilesCell typesOxytocin signaling pathwayPrefrontal cortexMotivated behaviorFrontal cortexDopaminergic pathwaysUse disorderBrain regionsModulation of chemical synaptic transmissionStriatumSingle-cell RNAseqCell-specific pathwaysBehavioral responsesScRNA-seqStriatum cells
2021
Candidate pharmacological treatments for substance use disorder and suicide identified by gene co‐expression network‐based drug repositioning
Cabrera‐Mendoza B, Martínez‐Magaña J, Monroy‐Jaramillo N, Genis‐Mendoza A, Fresno C, Fries GR, Walss‐Bass C, Armenta M, García‐Dolores F, Díaz‐Otañez C, Flores G, Vázquez‐Roque R, Nicolini H. Candidate pharmacological treatments for substance use disorder and suicide identified by gene co‐expression network‐based drug repositioning. American Journal Of Medical Genetics Part B Neuropsychiatric Genetics 2021, 186: 193-206. PMID: 33403748, DOI: 10.1002/ajmg.b.32830.Peer-Reviewed Original ResearchConceptsSubstance use disordersUse disordersBrain gene co-expression networksHub genesPotential pharmacological targetPost-mortem samplesPharmacological treatmentHigh riskCandidate treatmentPharmacological targetsSuicidal behaviorMAOA inhibitorsPrefrontal cortexNonsuicidesDGIdb databaseGene expression profilesPatientsCo-expression networkSuicideDisordersDrugsTreatmentDrug repositioningExpression profilesIndividuals
2018
Astrogliosis and decreased neural viability as consequences of early consumption of aspartame and acesulfame potassium in male Wistar rats
Solis-Medina A, Martínez-Magaña JJ, Quintanar-Jurado V, Gallegos-Silva I, Juárez-Rojop IE, Tovilla-Zárate CA, Díaz-Zagoya JC, Hernández-Díaz Y, González-Castro TB, López-Narváez ML, Genis-Mendoza AD, Nicolini H. Astrogliosis and decreased neural viability as consequences of early consumption of aspartame and acesulfame potassium in male Wistar rats. Metabolic Brain Disease 2018, 33: 2031-2038. PMID: 30264280, DOI: 10.1007/s11011-018-0310-7.Peer-Reviewed Original ResearchConceptsMale Wistar ratsNeuronal specific enolaseNeuronal viabilityWistar ratsPrefrontal cortexChronic consumptionNeural viabilityConsumption of aspartameHematoxylin-eosin stainingConsumption of sweetenersHistological alterationsAstrogliosisImmunohistochemical techniquesBrain areasControl groupNeural modificationsNeuronal morphologyPyknotic nucleiHistological analysisHippocampusAmygdalaRatsCortexEarly ageEarly consumption