2015
Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli
Chang WG, Fornoni A, Tietjen G, Mendez JJ, Niklason LE, Saltzman WM, Pober JS. Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli. Tissue Engineering Part A 2015, 21: 2673-2679. PMID: 26414101, PMCID: PMC4652181, DOI: 10.1089/ten.tea.2015.0060.Peer-Reviewed Original Research
2001
Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway
Mahboubi K, Li F, Plescia J, Kirkiles-Smith N, Mesri M, Du Y, Carroll J, Elias J, Altieri D, Pober J. Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway. Laboratory Investigation 2001, 81: 327-334. PMID: 11310826, DOI: 10.1038/labinvest.3780241.Peer-Reviewed Original ResearchMeSH KeywordsDNA-Binding ProteinsEndothelium, VascularGene ExpressionHumansInhibitor of Apoptosis ProteinsInterleukin-11Microtubule-Associated ProteinsNeoplasm ProteinsPhosphorylationProteinsRNA, MessengerSerineSignal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorSurvivinTrans-ActivatorsTranscription, GeneticTransgenesUmbilical VeinsConceptsSignal transducerProtein kinase B/AktDominant-negative STAT3 mutantActivator of transcriptionSurvivin mRNA expressionTranscription 3 (STAT3) pathwayIL-11Survivin protein expressionSTAT3 mutantStimulation of serumProtein expression levelsSurvivin inductionSurvivin expressionAntiapoptotic proteinsInduces expressionMRNA expressionExpression levelsSurvivin proteinProtein expressionCritical mediatorMaximal inductionExpressionSurvivin mRNAProteinDose-dependent manner
2000
A Phosphatidylinositol 3-Kinase/Akt Pathway, Activated by Tumor Necrosis Factor or Interleukin-1, Inhibits Apoptosis but Does Not Activate NFκB in Human Endothelial Cells*
Madge L, Pober J. A Phosphatidylinositol 3-Kinase/Akt Pathway, Activated by Tumor Necrosis Factor or Interleukin-1, Inhibits Apoptosis but Does Not Activate NFκB in Human Endothelial Cells*. Journal Of Biological Chemistry 2000, 275: 15458-15465. PMID: 10748004, DOI: 10.1074/jbc.m001237200.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesApoptosisCells, CulturedChromonesCulture Media, Serum-FreeEndothelium, VascularEnzyme ActivationEnzyme InhibitorsHumansInterleukin-1KineticsMorpholinesNF-kappa BPhosphatidylinositol 3-KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRecombinant ProteinsTumor Necrosis Factor-alphaUmbilical VeinsWortmanninConceptsHuman endothelial cellsProtein kinaseStress-activated protein kinaseAkt pathwayMitogen-activated protein kinaseProtein kinase AktPromoter-reporter geneInhibitor of phosphatidylinositolEndothelial cellsSerum-deprived endothelial cellsPhosphorylation of AktGrowth factorAnti-apoptotic pathwaysKinase AktGene productsAnti-apoptotic effectsInhibits ApoptosisSerum deprivationAktLY294002Phospho-AktPro-inflammatory gene productsTranscriptionKinasePhosphatidylinositolCytoprotection of Human Umbilical Vein Endothelial Cells Against Apoptosis and CTL-Mediated Lysis Provided by Caspase-Resistant Bcl-2 Without Alterations in Growth or Activation Responses
Zheng L, Dengler T, Kluger M, Madge L, Schechner J, Maher S, Pober J, Bothwell A. Cytoprotection of Human Umbilical Vein Endothelial Cells Against Apoptosis and CTL-Mediated Lysis Provided by Caspase-Resistant Bcl-2 Without Alterations in Growth or Activation Responses. The Journal Of Immunology 2000, 164: 4665-4671. PMID: 10779771, DOI: 10.4049/jimmunol.164.9.4665.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCaspasesCell DivisionCell Line, TransformedCells, CulturedCulture Media, ConditionedCytotoxicity, ImmunologicEndothelial Growth FactorsEndothelium, VascularGenetic VectorsGreen Fluorescent ProteinsHumansLuminescent ProteinsProto-Oncogene Proteins c-bcl-2RetroviridaeT-Lymphocytes, CytotoxicTransduction, GeneticTransfectionUmbilical VeinsConceptsGraft endothelial cellsAllograft rejectionBcl-2Endothelial cellsAcute allograft rejectionClass I MHC moleculesNF-kappaB activationHuman umbilical vein endothelial cellsI MHC moleculesUmbilical vein endothelial cellsHost CTLVein endothelial cellsEndothelial injuryAnti-apoptotic gene Bcl-2MHC moleculesGene Bcl-2Induction of apoptosisBcl-2-transduced cellsClass IActivation responseApoptotic effectsCTLHUVECTNFGrowth factor withdrawalIL-11 Activates Human Endothelial Cells to Resist Immune-Mediated Injury
Mahboubi K, Biedermann B, Carroll J, Pober J. IL-11 Activates Human Endothelial Cells to Resist Immune-Mediated Injury. The Journal Of Immunology 2000, 164: 3837-3846. PMID: 10725745, DOI: 10.4049/jimmunol.164.7.3837.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDCells, CulturedComplement System ProteinsCytokine Receptor gp130Cytotoxicity, ImmunologicDNA-Binding ProteinsDose-Response Relationship, ImmunologicEndothelium, VascularEnzyme ActivationHumansImmunity, InnateInflammation MediatorsInterleukin-11Interleukin-11 Receptor alpha SubunitMembrane GlycoproteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNF-kappa BPhosphorylationReceptors, InterleukinReceptors, Interleukin-11Signal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorT-Lymphocytes, CytotoxicTrans-ActivatorsTyrosineUmbilical VeinsConceptsIL-11Mitogen-activated protein kinaseP44 mitogen-activated protein kinaseImmune-Mediated InjuryCytolytic T lymphocytesNF-kappaB activationGp130-signaling cytokinesInflammatory injuryHuman endothelial cellsIL-11 receptorProinflammatory responseMolecule expressionT lymphocytesICAM-1Maximal responseE-selectinMHC AbsVivo modelNF-kappaBEndothelial cellsTyrosine phosphorylationPhospho-STAT3Cultured HUVECsInjuryKinase 1 inhibitor
1999
Human endothelial cells augment early CD40 ligand expression in activated CD4+ T cells through LFA-3-mediated stabilization of mRNA.
Murakami K, Ma W, Fuleihan R, Pober J. Human endothelial cells augment early CD40 ligand expression in activated CD4+ T cells through LFA-3-mediated stabilization of mRNA. The Journal Of Immunology 1999, 163: 2667-73. PMID: 10453007, DOI: 10.4049/jimmunol.163.5.2667.Peer-Reviewed Original ResearchConceptsCD40 ligand expressionT cellsCD40L mRNALFA-3CD40L expressionICAM-1Ligand expressionICAM-1 antisense oligonucleotideT-cell mRNA expressionCD40L protein expressionPretreatment of ECPromoter-reporter geneHuman endothelial cellsIL-2CD4Capacity of ECMRNA expressionICAM-2Endothelial cellsProtein expressionCD40LAntisense oligonucleotidePHAMRNACells
1998
Human endothelial cells induce and regulate cytolytic T cell differentiation.
Biedermann B, Pober J. Human endothelial cells induce and regulate cytolytic T cell differentiation. The Journal Of Immunology 1998, 161: 4679-87. PMID: 9794397, DOI: 10.4049/jimmunol.161.9.4679.Peer-Reviewed Original ResearchConceptsB lymphoblastoid cellsEndothelial cellsHuman endothelial cellsSame donorT cellsProliferation of CD8IFN-gamma secretionCultured human endothelial cellsT cell differentiationT cell growthAllogeneic CD8CTL responsesPerforin expressionCTL differentiationPresence of ECT lymphocytesAllospecific CTLCD8CTLEfficient stimulatorsLymphoblastoid cellsCell numberReproducible frequencyCell growthInput cell numberHuman endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells.
Ma W, Pober J. Human endothelial cells effectively costimulate cytokine production by, but not differentiation of, naive CD4+ T cells. The Journal Of Immunology 1998, 161: 2158-67. PMID: 9725207, DOI: 10.4049/jimmunol.161.5.2158.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAdultCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell DifferentiationCells, CulturedCytokinesEndothelium, VascularHumansImmunologic MemoryInterferon-gammaInterleukin-12Interleukin-2Interleukin-4InterphaseLeukocytes, MononuclearLymphocyte ActivationTh1 CellsT-Lymphocyte SubsetsUmbilical VeinsConceptsPeripheral blood adherent mononuclear cellsExogenous IL-12Naive T cellsT cellsIL-12Endothelial cellsMemory CD4Human endothelial cellsCytokine productionNaive CD4B7 moleculesIL-2IFN-gammaBone marrow-derived APCsBlood adherent mononuclear cellsB7 molecule expressionIL-12 supplementationIntracellular cytokine stainingTh1-like cellsIL-4 productionIFN-gamma productionCytokine stainingMononuclear cellsIL-4Molecule expressionThe N-terminal domains target TNF receptor-associated factor-2 to the nucleus and display transcriptional regulatory activity.
Min W, Bradley JR, Galbraith JJ, Jones SJ, Ledgerwood EC, Pober JS. The N-terminal domains target TNF receptor-associated factor-2 to the nucleus and display transcriptional regulatory activity. The Journal Of Immunology 1998, 161: 319-24. PMID: 9647239, DOI: 10.4049/jimmunol.161.1.319.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportCell NucleusCells, CulturedCytoplasmEndothelium, VascularFluorescent Antibody Technique, IndirectHumansPeptide FragmentsProtein BiosynthesisProtein Structure, TertiaryProteinsReceptors, Tumor Necrosis FactorTNF Receptor-Associated Factor 2Transcription, GeneticTransfectionUmbilical VeinsConceptsFinger domainAmino-terminal RING finger domainNuclear localizationTNF receptor-associated factor 2Cytoplasmic signal transductionReceptor-associated factor 2Zinc finger domainTranscriptional regulatory activityAmino-terminal halfC-Jun N-terminal kinase (JNK) activationRING finger domainProminent nuclear localizationConfocal immunofluorescence microscopyWestern blottingTRAF2 moleculeAdaptor proteinDeletion mutantsSignal transductionSubcellular localizationGene transcriptionKinase activationHuman endothelial cellsTRAF2 proteinCell extractsHuman endothelial cell lineEndothelial cells in physiology and in the pathophysiology of vascular disorders.
Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, Pober JS, Wick TM, Konkle BA, Schwartz BS, Barnathan ES, McCrae KR, Hug BA, Schmidt AM, Stern DM. Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 1998, 91: 3527-61. PMID: 9572988.Peer-Reviewed Original ResearchAnimalsArteriosclerosisBlood CellsBlood CoagulationCell AdhesionCell Adhesion MoleculesCell DifferentiationCells, CulturedEndothelial Growth FactorsEndothelinsEndothelium, VascularEpoprostenolExtracellular MatrixFemaleFibrinolysisFibroblast Growth Factor 2HemodynamicsHumansInflammationLymphokinesMaleMiceNeovascularization, PhysiologicNitric OxidePlatelet Activating FactorPre-EclampsiaPregnancyThrombosisUmbilical VeinsVascular DiseasesVascular Endothelial Growth Factor AVascular Endothelial Growth Factors
1997
TNF initiates E-selectin transcription in human endothelial cells through parallel TRAF-NF-kappa B and TRAF-RAC/CDC42-JNK-c-Jun/ATF2 pathways.
Min W, Pober JS. TNF initiates E-selectin transcription in human endothelial cells through parallel TRAF-NF-kappa B and TRAF-RAC/CDC42-JNK-c-Jun/ATF2 pathways. The Journal Of Immunology 1997, 159: 3508-18. PMID: 9317150, DOI: 10.4049/jimmunol.159.7.3508.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Antigens, SurfaceCalcium-Calmodulin-Dependent Protein KinasesCyclic AMP Response Element-Binding ProteinEndothelium, VascularE-SelectinGTP-Binding ProteinsHumansJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesNF-kappa BPhosphorylationPromoter Regions, GeneticProto-Oncogene Proteins c-junRac GTP-Binding ProteinsReceptors, Tumor Necrosis FactorTranscription FactorsTranscription, GeneticTumor Necrosis Factor Receptor-Associated Peptides and ProteinsTumor Necrosis Factor-alphaUmbilical VeinsConceptsTransient overexpressionHuman endothelial cellsC-JunSelectin transcriptionATF2/c-JunN-terminalATF2 pathwayPromoter-reporter geneKappa B elementCAMP-responsive elementEndothelial cellsDomain phosphorylationEndogenous JNKAdaptor proteinInactive JNKNF-kappa BGene transcriptionGene promoterKappa BJNK activationKinase 1TranscriptionTRAF2 proteinCdc42JNK
1996
Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: cloning, sequencing, and functional expression in human endothelial cells.
Maher SE, Karmann K, Min W, Hughes CC, Pober JS, Bothwell AL. Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: cloning, sequencing, and functional expression in human endothelial cells. The Journal Of Immunology 1996, 157: 3838-44. PMID: 8892613, DOI: 10.4049/jimmunol.157.9.3838.Peer-Reviewed Original ResearchAbataceptAmino Acid SequenceAnimalsAntigens, CDAntigens, DifferentiationAortaB7-2 AntigenBase SequenceCells, CulturedCHO CellsCloning, MolecularCricetinaeCricetulusCTLA-4 AntigenDNA, ComplementaryEndothelium, VascularHumansImmunoconjugatesInterleukin-2Lymphocyte ActivationMembrane GlycoproteinsMolecular Sequence DataSequence AlignmentSequence Homology, Amino AcidSpecies SpecificitySwineT-LymphocytesTransfectionUmbilical VeinsProlonged cytokine exposure causes a dynamic redistribution of endothelial cell adhesion molecules to intercellular junctions.
Bradley JR, Pober JS. Prolonged cytokine exposure causes a dynamic redistribution of endothelial cell adhesion molecules to intercellular junctions. Laboratory Investigation 1996, 75: 463-72. PMID: 8874379.Peer-Reviewed Original ResearchConceptsCell junctionsProtein redistributionMembrane protein redistributionLongitudinal stress fibersDynamic redistributionIntercellular adhesion molecule-2Cultured umbilical vein endothelial cellsBeta 3 integrinAdhesion moleculesBeta 1 integrinCell adhesion moleculeDense peripheral bandStress fibersCytoskeletal actinEndothelial cellsUmbilical vein endothelial cellsActin filamentsCell adhesion molecule-1Vein endothelial cellsAdhesion molecule-1Intercellular adhesion molecule 1 moleculesIntercellular junctionsApical surfaceVascular cell adhesion molecule-1Peripheral bandA Sustained Reduction in IκB-β May Contribute to Persistent NF-κB Activation in Human Endothelial Cells*
Johnson D, Douglas I, Jahnke A, Ghosh S, Pober J. A Sustained Reduction in IκB-β May Contribute to Persistent NF-κB Activation in Human Endothelial Cells*. Journal Of Biological Chemistry 1996, 271: 16317-16322. PMID: 8663191, DOI: 10.1074/jbc.271.27.16317.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesCell Adhesion MoleculesCell MembraneCell NucleusCells, CulturedConsensus SequenceCytosolDNADNA-Binding ProteinsEndothelium, VascularGene ExpressionHistocompatibility Antigens Class IHumansI-kappa B ProteinsIntercellular Adhesion Molecule-1Interleukin-1KineticsMolecular Sequence DataNF-kappa BOligodeoxyribonucleotidesTetradecanoylphorbol AcetateTumor Necrosis Factor-alphaUmbilical VeinsVascular Cell Adhesion Molecule-1ConceptsAdhesion molecule-1Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Phorbol myristate acetateInterleukin-1alphaNF-kappaB activationMolecule-1Sustained reductionNF-kappaBEndothelial cellsIkappaB-alphaIkappaB-betaHuman endothelial cellsHuman leukocyte antigen (HLA) class INF-kappaB.Inhibitory protein IkappaB-alphaCell surface molecule expressionIkappaB-beta degradationPersistent NF-κB activationSurface molecule expressionAntigen class INF-κB activationIkappaB-beta levelsDe novo expression
1995
CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.
Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS. CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4342-4346. PMID: 7538666, PMCID: PMC41940, DOI: 10.1073/pnas.92.10.4342.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, B-LymphocyteCD40 AntigensCell Adhesion MoleculesCells, CulturedCytokinesEndothelium, VascularGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorHumansInterferon-betaInterferon-gammaInterleukinsKineticsMiceRecombinant ProteinsSkinTumor Necrosis Factor-alphaUmbilical VeinsConceptsTumor necrosis factorAdhesion molecule-1Endothelial cellsHuman endothelial cellsNecrosis factorInterleukin-1Molecule-1T cell-mediated inflammatory reactionVascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Cultured human umbilical vein endothelial cellsAdhesion molecule expressionLeukocyte adhesion moleculesMicrovascular endothelial cellsHuman microvascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsFlow cytometric quantitationVein endothelial cellsCD40 antigenEndothelial CD40Interferon betaMolecule expressionInflammatory reactionVariability Among Human Umbilical Vein Endothelial Cultures
Watson C, Camera-Benson L, Palmer-Crocker R, Pober J. Variability Among Human Umbilical Vein Endothelial Cultures. Science 1995, 268: 447-448. PMID: 7716553, DOI: 10.1126/science.7716553.Peer-Reviewed Original ResearchIL-4 induction of VCAM-1 on endothelial cells involves activation of a protein tyrosine kinase.
Palmer-Crocker RL, Pober JS. IL-4 induction of VCAM-1 on endothelial cells involves activation of a protein tyrosine kinase. The Journal Of Immunology 1995, 154: 2838-45. PMID: 7533184, DOI: 10.4049/jimmunol.154.6.2838.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1VCAM-1 expressionEndothelial cellsIL-4 inductionIL-4IL-4R.Cell adhesion molecule-1Pretreatment of ECTreatment of ECIL-4 concentrationsAdhesion molecule-1Cultured human endothelial cellsHerbimycin ATime of treatmentHuman endothelial cellsTyrosine kinaseMolecule-1Western blotProtein tyrosine kinasesTyrosine phosphorylationIL-4-induced tyrosine phosphorylationAnti-phosphotyrosine immunoprecipitatesReplicate wellsDisparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells.
Bradley JR, Thiru S, Pober JS. Disparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells. American Journal Of Pathology 1995, 146: 27-32. PMID: 7856733, PMCID: PMC1870772.Peer-Reviewed Original ResearchConceptsTumor necrosis factor receptorNecrosis factor receptorCell surfaceFactor receptorConfocal immunofluorescence microscopyDisparate localizationUndergoes endocytosisCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsEndothelial cellsUmbilical vein endothelial cellsReceptor clusteringGolgi apparatusHuman endothelial cellsCoated vesiclesVein endothelial cellsImmunofluorescence microscopyCellular distributionTNF receptorEndothelial cell activationCell activationReceptorsCytoplasmic vacuolesCellsEndocytosis
1994
Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells
Birch K, Ewenstein B, Golan D, Pober J. Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells. Journal Of Cellular Physiology 1994, 160: 545-554. PMID: 7521337, DOI: 10.1002/jcp.1041600318.Peer-Reviewed Original ResearchConceptsEndothelial cellsAgonist concentrationsSingle human umbilical vein endothelial cellCytoplasmic free calcium ionsNumber of cellsPeak elevationHigh agonist concentrationsCytoplasmic free calciumHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVWF secretionIndividual endothelial cellsAmount of secretionVein endothelial cellsHuman endothelial cellsAgonist treatmentVon Willebrand factor secretionAgonist doseExtracellular Ca2Maximal secretionP-selectinThrombin receptorHigh dosesIntracellular storesFactor secretion
1993
HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM
SAVAGE C, HUGHES C, MCINTYRE B, PICARD J, POBER J. HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM. Transplantation 1993, 56: 128-134. PMID: 7687392, DOI: 10.1097/00007890-199307000-00024.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, T-LymphocyteCD2 AntigensCD4 AntigensCD58 AntigensCells, CulturedEndothelium, VascularFlow CytometryHistocompatibility Antigens Class IHLA-DR AntigensHumansInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsPolymerase Chain ReactionReceptors, ImmunologicRecombinant ProteinsT-Lymphocyte SubsetsTransplantation, HomologousUmbilical VeinsConceptsHLA-DR expressing cellsT cellsHLA-DRPolymerase chain reactionEC culturesAllogeneic class II MHC moleculesMHC moleculesLFA-1 beta chainLFA-1Peripheral blood CD4VLA-4 alphaMHC class II moleculesT cell responsesClass II MHC moleculesT cell surface moleculesT cell proliferationClass I MHC moleculesExpressing cellsClass II moleculesBeta chainII MHC moleculesDe novo expressionI MHC moleculesAllogeneic proliferationBlood CD4