2000
Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype
Dengler T, Pober J. Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype. The Journal Of Immunology 2000, 164: 5146-5155. PMID: 10799873, DOI: 10.4049/jimmunol.164.10.5146.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCoculture TechniquesCyclosporineCytotoxicity Tests, ImmunologicEndothelium, VascularHistocompatibility Antigens Class IHumansImmunologic MemoryImmunophenotypingInterleukin-12InterphaseLeukocyte Common AntigensLymphocyte ActivationT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicConceptsB lymphoblastoid cellsT cellsNaive CD8Endothelial cellsImmunoregulatory cell typesIntracellular perforin contentAlloreactive T cellsAnti-CD28 mAbHuman vascular endothelial cellsHigh surface expressionVascular endothelial cellsExpansion of memoryConventional CTLGraft parenchymaGraft rejectionMemory CD8CTL generationPerforin contentCTL expansionVascular injuryHuman CD8CD40 ligandAlloreactive CTLAnatomic compartmentsICAM-1
1999
Human vascular endothelial cells favor clonal expansion of unusual alloreactive CTL.
Biedermann B, Pober J. Human vascular endothelial cells favor clonal expansion of unusual alloreactive CTL. The Journal Of Immunology 1999, 162: 7022-30. PMID: 10358144, DOI: 10.4049/jimmunol.162.12.7022.Peer-Reviewed Original ResearchConceptsTarget cell contactB lymphoblastoid cellsLittle IFN-gammaIFN-gammaEndothelial cellsCTL clonesClass I MHC-restricted CTLAbsence of ECExpression of CD40LAllogeneic endothelial cellsIFN-gamma secretionIL-4 secretionCell contactHuman vascular endothelial cellsVascular endothelial cellsCTL subsetsCD40L expressionCTL linesCD40 ligandT cellsAlloreactive CTLArterial intimaClonal expansionCTLCultured HUVECs
1997
Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40–CD40 ligand signaling in atherosclerosis
Mach F, Schönbeck U, Sukhova G, Bourcier T, Bonnefoy J, Pober J, Libby P. Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40–CD40 ligand signaling in atherosclerosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1931-1936. PMID: 9050882, PMCID: PMC20020, DOI: 10.1073/pnas.94.5.1931.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteArteriosclerosisBlotting, WesternB-LymphocytesCD40 AntigensCD40 LigandCells, CulturedEndothelium, VascularFlow CytometryGene Expression RegulationHumansImmunohistochemistryInterferon-gammaInterleukin-1MacrophagesMembrane GlycoproteinsMuscle, Smooth, VascularRNA, MessengerSignal TransductionTumor Necrosis Factor-alphaConceptsHuman vascular endothelial cellsSmooth muscle cellsVascular endothelial cellsHuman atherosclerotic lesionsHuman macrophagesCell typesEndothelial cellsMuscle cellsHuman vascular smooth muscle cellsVascular smooth muscle cellsDe novo synthesisCD40 ligandBroad functionsAtherosclerotic lesionsCD40 SignalingTumor necrosis factor alphaFunctional CD40 ligandInvolvement of inflammationCultured human vascular endothelial cellsCD40-CD40 ligandNovo synthesisNecrosis factor alphaParacrine activationNormal arterial tissueNovel source
1996
Ceramide Is Not a Signal for Tumor Necrosis Factor–Induced Gene Expression but Does Cause Programmed Cell Death in Human Vascular Endothelial Cells
Slowik M, De Luca L, Min W, Pober J. Ceramide Is Not a Signal for Tumor Necrosis Factor–Induced Gene Expression but Does Cause Programmed Cell Death in Human Vascular Endothelial Cells. Circulation Research 1996, 79: 736-747. PMID: 8831497, DOI: 10.1161/01.res.79.4.736.Peer-Reviewed Original ResearchConceptsConcentrations of TNFNF-kappa BEndothelial cellsJun N-terminal kinaseNecrosis factorEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Treatment of ECCell deathCultured human umbilical vein endothelial cellsEndothelial leukocyte adhesion moleculeTumor necrosis factorAdhesion molecule-1Leukocyte adhesion moleculesHuman vascular endothelial cellsHuman umbilical vein endothelial cellsStimulation of ECUmbilical vein endothelial cellsVascular endothelial cellsP50/p65 heterodimeric formVein endothelial cellsPotential intracellular mediatorsIL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma c chain.
Palmer-Crocker RL, Hughes CC, Pober JS. IL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma c chain. Journal Of Clinical Investigation 1996, 98: 604-609. PMID: 8698849, PMCID: PMC507467, DOI: 10.1172/jci118829.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCells, CulturedEndothelium, VascularHumansInterleukin-3Interleukin-4Janus Kinase 2Molecular Sequence DataPhosphorylationProtein-Tyrosine KinasesProto-Oncogene ProteinsReceptors, Interleukin-2RNA, MessengerSTAT6 Transcription FactorTrans-ActivatorsTyrosineVascular Cell Adhesion Molecule-1ConceptsIL-4 responsesIL-13IL-4Human endothelial cellsEndothelial cellsVascular cell adhesion molecule-1Cell adhesion molecule-1Cultured human vascular endothelial cellsAdhesion molecule-1IL-15 receptorTyrosine kinaseCommon signaling subunitHuman vascular endothelial cellsJAK2 tyrosine kinaseVascular endothelial cellsSTAT6 transcription factorReverse transcription-PCR methodGamma c chainTranscription-PCR methodGC-independent pathwayIL-2IL-9IL-7IL-4RMolecule-1
1985
Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion.
Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA. Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion. American Journal Of Pathology 1985, 121: 394-403. PMID: 3878084, PMCID: PMC1887931.Peer-Reviewed Original ResearchConceptsInterleukin-1Procoagulant activityHuman endothelial cellsEndothelial cellsVascular endotheliumTissue factor-like procoagulant activityLeukocyte adhesionPeripheral blood polymorphonuclear leukocytesHuman peripheral blood polymorphonuclear leukocytesCultured human vascular endothelial cellsBlood polymorphonuclear leukocytesIL-1 actionIL-1 effectsRecombinant IL-1Sites of inflammationHuman vascular endothelial cellsInterleukin-1 activationVascular endothelial cellsEnhanced leukocyteIntravascular coagulationImmune mediatorsIL-2Saphenous veinEndothelial cell surfaceUmbilical vein
1984
Interactions of T Lymphocytes with Human Vascular Endothelial Cells: Role of Endothelial Cells Surface Antigens
Pober JS, Gimbrone MA, Collins T, Cotran RS, Ault KA, Fiers W, Krensky AM, Clayberger C, Reiss C, Burakoff SJ. Interactions of T Lymphocytes with Human Vascular Endothelial Cells: Role of Endothelial Cells Surface Antigens. Immunobiology 1984, 168: 483-494. PMID: 6397428, DOI: 10.1016/s0171-2985(84)80132-1.Peer-Reviewed Original ResearchConceptsClass II antigensVascular endothelial cellsMajor histocompatibility complexII antigensT cellsClass II moleculesHuman vascular endothelial cellsActivated T cellsEndothelial cellsStromal cellsImmune interferonT lymphocytesAntigen-specific T cellsPeripheral blood T lymphocytesClass IHelper T cell clonesImmune accessory functionNon-MHC antigensWithdrawal of interferonClass II major histocompatibility complexCultured human vascular endothelial cellsT cell responsesVascular smooth muscle cellsBlood T lymphocytesHuman class II antigensInterleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells.
Bevilacqua MP, Pober JS, Majeau GR, Cotran RS, Gimbrone MA. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells. Journal Of Experimental Medicine 1984, 160: 618-623. PMID: 6332168, PMCID: PMC2187463, DOI: 10.1084/jem.160.2.618.Peer-Reviewed Original ResearchConceptsEndothelial cell procoagulant activityCell procoagulant activityProcoagulant activityInterleukin-1Cultured human vascular endotheliumHuman monocyte-derived interleukin 1Coagulation factor-deficient plasmasMonocyte-derived interleukin 1Human vascular endothelial cellsHuman vascular endotheliumVascular endothelial cellsFactor-deficient plasmaIL-1 inductionIntravascular coagulationInflammatory conditionsCell surface expressionIntact cell monolayersVascular endotheliumEndothelial cellsPotent inducerSurface expressionCell monolayersProtein synthesisPathogenesisActivity
1982
Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation.
Pober JS, Gimbrone MA. Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6641-6645. PMID: 6815654, PMCID: PMC347184, DOI: 10.1073/pnas.79.21.6641.Peer-Reviewed Original ResearchConceptsHuman vascular endothelial cellsCell shape changesVascular endothelial cellsEndothelial cellsMembrane proteinsCell divisionRadioiodinated membrane proteinsHuman endothelial cellsStandard culture conditionsIntact cellsCultured human endothelial cellsBiochemical demonstrationImmunoprecipitationCulture conditionsConfluent culturesExpressionMonoclonal antibody bindingCellsPrimary culturesShape changesMonoclonal antibodiesLectin phytohemagglutininProteinRegulationImportant implications