2016
Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis
Telles CJ, Decker SE, Motley WW, Peters AW, Mehr AP, Frizzell RA, Forrest JN. Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis. American Journal Of Physiology - Cell Physiology 2016, 311: c884-c894. PMID: 27653983, PMCID: PMC5206301, DOI: 10.1152/ajpcell.00030.2016.Peer-Reviewed Original ResearchConceptsShark rectal glandRectal glandCFTR channelsConfocal immunofluorescent microscopyChloride secretionTASK-1 potassium channelFour-transmembraneApical chloride secretionMammalian lung tissuePotassium channelsGenomic walkingTwo-electrode voltage clampingTASK-1 proteinRapid amplificationDegenerate primersMolecular identificationMolecular identityHuman biologyAmino acidsXenopus oocytesHuman TASK-1ProteinTASK-1 channelsHuman airway cellsIdentical current-voltage relationships
2006
Shark rectal gland vasoactive intestinal peptide receptor: cloning, functional expression, and regulation of CFTR chloride channels
Bewley MS, Pena JT, Plesch FN, Decker SE, Weber GJ, Forrest JN. Shark rectal gland vasoactive intestinal peptide receptor: cloning, functional expression, and regulation of CFTR chloride channels. AJP Regulatory Integrative And Comparative Physiology 2006, 291: r1157-r1164. PMID: 16728467, DOI: 10.1152/ajpregu.00078.2006.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell Cycle ProteinsChloridesCloning, MolecularConserved SequenceCystic Fibrosis Transmembrane Conductance RegulatorDogfishEndodeoxyribonucleasesGene Expression RegulationIn Vitro TechniquesMaleMolecular Sequence DataOocytesPatch-Clamp TechniquesPhylogenyReceptors, Vasoactive Intestinal PeptideReverse Transcriptase Polymerase Chain ReactionSalt GlandVasoactive Intestinal PeptideXenopus laevisConceptsShark rectal glandCFTR chloride channelRectal glandVasoactive intestinal peptideChloride channelsN-glycosylation sitesG protein-coupled receptorsUnique G protein-coupled receptorProtein-coupled receptorsChloride conductanceCysteine residuesNH2 terminusChloride secretionFunctional expressionGrowth hormone-releasing hormoneCFTR mRNADogfish sharkAmino acidsPeptide histidine isoleucine amideRelative potencyXenopus oocytesHormone-releasing hormoneHistidine isoleucine amideQuantitative PCRMicroeq x
1991
Cl- secretion by cultured shark rectal gland cells. I. Transepithelial transport
Valentich JD, Forrest JN. Cl- secretion by cultured shark rectal gland cells. I. Transepithelial transport. American Journal Of Physiology 1991, 260: c813-c823. PMID: 2018113, DOI: 10.1152/ajpcell.1991.260.4.c813.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptideCl- secretory epitheliaPrimary monolayer culture systemCyclic monophosphate (cAMP) accumulationShark rectal gland (SRG) epithelial cellsIntestinal peptidePotent secretagogueGland epithelial cellsAutocrine mediatorMonophosphate accumulationCultured shark rectal gland cellsEpithelial cells
1990
A1 adenosine receptors inhibit chloride transport in the shark rectal gland. Dissociation of inhibition and cyclic AMP.
Kelley GG, Poeschla EM, Barron HV, Forrest JN. A1 adenosine receptors inhibit chloride transport in the shark rectal gland. Dissociation of inhibition and cyclic AMP. Journal Of Clinical Investigation 1990, 85: 1629-1636. PMID: 1970583, PMCID: PMC296615, DOI: 10.1172/jci114614.Peer-Reviewed Original Research