2024
Glycerol Kinase Deficiency with Increased Triglycerides and Weight Gain: Pseudo or Real?
Jiao J, Bernard P, Meeusen J, Militello L, El-Khoury J. Glycerol Kinase Deficiency with Increased Triglycerides and Weight Gain: Pseudo or Real? Clinical Chemistry 2024, 70: 703-706. PMID: 38692658, DOI: 10.1093/clinchem/hvae032.Peer-Reviewed Original Research
2022
Association of renalase with clinical outcomes in hospitalized patients with COVID-19
Safdar B, Wang M, Guo X, Cha C, Chun HJ, Deng Y, Dziura J, El-Khoury JM, Gorelick F, Ko AI, Lee AI, Safirstein R, Simonov M, Zhou B, Desir GV. Association of renalase with clinical outcomes in hospitalized patients with COVID-19. PLOS ONE 2022, 17: e0264178. PMID: 35259186, PMCID: PMC8903289, DOI: 10.1371/journal.pone.0264178.Peer-Reviewed Original ResearchConceptsCOVID-19 patientsRenalase levelsIntensive care unit admissionHospitalized COVID-19 patientsMean age 64 yearsCOVID-19Cox proportional hazards modelCare unit admissionPrimary composite outcomeRetrospective cohort studyUse of vasopressorsSevere COVID-19IL-6 levelsAge 64 yearsRisk of deathCOVID-19 subjectsInitial disease severityProportional hazards modelCOVID-19 diseasePlasma renalaseUnit admissionICU admissionCohort studyComposite outcomeCytokine levels
2021
The ALT upper reference interval debate: Blame it on the alcohol
Vera MA, Koch CD, Liapakis A, Lim JK, El-Khoury JM. The ALT upper reference interval debate: Blame it on the alcohol. Clinica Chimica Acta 2021, 526: 62-65. PMID: 34973184, DOI: 10.1016/j.cca.2021.12.026.Peer-Reviewed Original ResearchConceptsUpper reference limitAdult groupHigher upper reference limitLiver diseasePediatric groupPediatric GastroenterologyPediatric populationClinical guidelinesALT valuesAmerican CollegeDerivation studyAge groupsReference limitsNorth American SocietyAmerican SocietyClinical laboratoriesRoche Cobas 8000GastroenterologyLaboratory expertsWomenCobas 8000MenGroupVariable inclusionIndividualsDetermination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population
Vera MA, Koch CD, Kavsak PA, El-Khoury JM. Determination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population. Clinica Chimica Acta 2021, 523: 397-401. PMID: 34666029, DOI: 10.1016/j.cca.2021.10.011.Peer-Reviewed Original ResearchConceptsHeart-type fatty acid-binding proteinUpper reference limitPercentile upper reference limitClinical utilityReference limitsAcute kidney injuryHeart-type fatty acidAcute coronary syndromeAACC Universal Sample BankFatty acid-binding proteinH-FABP concentrationHealthy U.S.Coronary syndromeFinal sample populationKidney injuryAssay cutoffHealthy donorsAcid-binding proteinUS populationImmunoturbidimetric assayPotential biomarkersClinical sensitivityMyocardial tissueSerum samplesLow molecular weight proteinsPerimenopausal woman with elevated serum hCG and abdominal pain
Li P, Koch CD, El-Khoury JM. Perimenopausal woman with elevated serum hCG and abdominal pain. Clinica Chimica Acta 2021, 522: 141-143. PMID: 34418366, DOI: 10.1016/j.cca.2021.08.018.Peer-Reviewed Original Research
2020
First- and Second-Trimester Reference Intervals for Thyroid Function Testing in a US Population
Bunch DR, Firmender K, Harb R, El-Khoury JM. First- and Second-Trimester Reference Intervals for Thyroid Function Testing in a US Population. American Journal Of Clinical Pathology 2020, 155: 776-780. PMID: 33210132, DOI: 10.1093/ajcp/aqaa165.Peer-Reviewed Original ResearchConceptsThyroid-stimulating hormoneTrimester-specific reference intervalsThyroid function testsMIU/LPmol/LReference intervalsFunction testsUS populationSecond trimester prenatal screeningRoche analyzersThyroid function testingNegative screening resultsSpecific reference intervalsRoche Modular E170Singleton pregnanciesThyroglobulin antibodiesThyroid dysfunctionFirst trimesterSecond trimesterAdverse outcomesFunction testingFree thyroxineTotal triiodothyronineTotal thyroxineModular E170Uromodulin to Osteopontin Ratio in Deceased Donor Urine Is Associated With Kidney Graft Outcomes.
Mansour SG, Liu C, Jia Y, Reese PP, Hall IE, El-Achkar TM, LaFavers KA, Obeid W, Rosenberg AZ, Daneshpajouhnejad P, Doshi MD, Akalin E, Bromberg JS, Harhay MN, Mohan S, Muthukumar T, Schröppel B, Singh P, El-Khoury JM, Weng FL, Thiessen-Philbrook HR, Parikh CR. Uromodulin to Osteopontin Ratio in Deceased Donor Urine Is Associated With Kidney Graft Outcomes. Transplantation 2020, 105: 876-885. PMID: 32769629, PMCID: PMC8805736, DOI: 10.1097/tp.0000000000003299.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsBiomarkersCells, CulturedDelayed Graft FunctionFemaleGlomerular Filtration RateGraft SurvivalHistocompatibility Antigens Class IIHumansKidneyKidney TransplantationMacrophagesMaleMiceMiddle AgedOsteopontinProspective StudiesRisk AssessmentRisk FactorsTime FactorsTissue DonorsUnited StatesUromodulinConceptsGraft failureGraft functionGraft outcomePrimary outcomeMajor histocompatibilityDeath-censored graft failureDeceased donor kidney transplantationCause graft failureImproved graft survivalRecipient graft outcomesKidney graft outcomeGlomerular filtration rateDeceased donor kidneysTime of procurementGraft survivalKidney transplantationDirection of associationDeceased donorsFiltration rateKidney qualityLower riskExtensive injuryDonor urineSimilar associationMouse macrophagesThe 2018 AACC/SYCL PhD Clinical Chemist Compensation Survey
Bunch DR, El-Khoury JM, Colby J, Colon-Franco J, Hackenmueller SA, Olson KN, Cotten SW, Kampfrath T, Chittiprol S, Suh-Lailam B, French D, Isbell TS, Nerenz RD, Parnas ML, Tolan NV. The 2018 AACC/SYCL PhD Clinical Chemist Compensation Survey. The Journal Of Applied Laboratory Medicine 2020, 5: 377-387. PMID: 32445390, DOI: 10.1093/jalm/jfaa001.Peer-Reviewed Original Research
2019
The Role of Volume Regulation and Thermoregulation in AKI during Marathon Running
Mansour SG, Martin TG, Obeid W, Pata RW, Myrick KM, Kukova L, Jia Y, Bjornstad P, El-Khoury JM, Parikh CR. The Role of Volume Regulation and Thermoregulation in AKI during Marathon Running. Clinical Journal Of The American Society Of Nephrology 2019, 14: 1297-1305. PMID: 31413064, PMCID: PMC6730516, DOI: 10.2215/cjn.01400219.Peer-Reviewed Original ResearchConceptsCore body temperatureBody temperaturePercent of runnersAcute tubular injuryPlasma copeptin concentrationsMajority of runnersContinuous core body temperatureAKIN criteriaDays postmarathonPrior marathonsTransient AKICopeptin concentrationsTubular injuryInjury biomarkersPrimary outcomeProspective cohortNephron damageNegative sodiumUrine microscopyVolume lossUrine sedimentAKIAbstractTextSweat sodiumCopeptinComparison of Urine and Plasma Biomarker Concentrations Measured by Aptamer-Based versus Immunoassay Methods in Cardiac Surgery Patients
Kukova LZ, Mansour SG, Coca SG, de Fontnouvelle CA, Thiessen-Philbrook HR, Shlipak MG, El-Khoury JM, Parikh CR. Comparison of Urine and Plasma Biomarker Concentrations Measured by Aptamer-Based versus Immunoassay Methods in Cardiac Surgery Patients. The Journal Of Applied Laboratory Medicine 2019, 4: 331-342. PMID: 31659071, DOI: 10.1373/jalm.2018.028621.Peer-Reviewed Original ResearchConceptsCardiac surgery patientsSurgery patientsCardiac surgeryBiomarker concentrationsAcute kidney injuryComparison of urinePlasma biomarker concentrationsImmunoassay methodPostoperative biomarkersPostoperative plasmaPreoperative biomarkersKidney injuryInjury cohortBiomarker discoveryBiomarker endpointsClinical settingCurrent immunoassay methodsBiomarker valuesUrine samplesTranslational researchUrineSpearman correlationPaired samplesPatientsSurgery
2017
Urinalysis findings and urinary kidney injury biomarker concentrations
Nadkarni GN, Coca SG, Meisner A, Patel S, Kerr KF, Patel UD, Koyner JL, Garg AX, Philbrook HT, Edelstein CL, Shlipak M, El-Khoury J, Parikh CR, on behalf of the TRIBE-AKI Consortium Investigators. Urinalysis findings and urinary kidney injury biomarker concentrations. BMC Nephrology 2017, 18: 218. PMID: 28683730, PMCID: PMC5499057, DOI: 10.1186/s12882-017-0629-z.Peer-Reviewed Original ResearchConceptsKidney injury molecule-1Post-operative samplesKidney injuryUrinalysis findingsUrinary biomarkersBiomarker concentrationsPost-operative AKIPre-operative samplesUrine dipstick testingPost-operative time pointsInjury molecule-1Time pointsRenal tubular damageUrinary biomarker concentrationsUrinary biomarker levelsTRIBE-AKI cohortLiver fatty acidClinical AKIDipstick proteinuriaProteinuria concentrationsUrinary NGALAKI biomarkersTubular damageCardiac surgeryNeutrophil gelatinase
2016
Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function
El-Khoury JM, Bunch DR, Hu B, Payto D, Reineks EZ, Wang S. Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function. Clinical Biochemistry 2016, 49: 1140-1143. PMID: 27452178, DOI: 10.1016/j.clinbiochem.2016.07.009.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateKidney functionCystatin CEGFR equationsWidespread clinical useIothalamate clearanceEGFR formulasFiltration rateSymmetric dimethylargininePlasma specimensAdult populationStrong markerAbstractTextClinical useEndogenous markerCreatinineLiquid chromatography-tandem mass spectrometry methodChromatography-tandem mass spectrometry methodFurther studiesDimethylarginineRoche Cobas 8000MarkersCatabolic productsCobas 8000
2012
Highly sensitive and selective measurement of underivatized methylmalonic acid in serum and plasma by liquid chromatography-tandem mass spectrometry
Yuan C, Gabler J, El-Khoury JM, Spatholt R, Wang S. Highly sensitive and selective measurement of underivatized methylmalonic acid in serum and plasma by liquid chromatography-tandem mass spectrometry. Analytical And Bioanalytical Chemistry 2012, 404: 133-140. PMID: 22618327, DOI: 10.1007/s00216-012-6099-z.Peer-Reviewed Original ResearchConceptsLC-MS/MS methodSample preparationMS methodAnion exchange solid phase extractionChromatography-tandem mass spectrometry methodLiquid chromatography-tandem mass spectrometry methodNegative electrospray modeSuccinic acidOrganic acid columnStrong anion-exchange solid-phase extractionSolid-phase extraction
2011
A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized l-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges
El-Khoury JM, Bunch DR, Reineks E, Jackson R, Steinle R, Wang S. A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized l-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges. Analytical And Bioanalytical Chemistry 2011, 402: 771-779. PMID: 22124751, DOI: 10.1007/s00216-011-5462-9.Peer-Reviewed Original ResearchConceptsFast liquid chromatography–tandem mass spectrometry methodAnalytical cycle timeChromatography-tandem mass spectrometry methodLiquid chromatography-tandem mass spectrometry methodMinimum sample preparationMass spectrometry methodLiquid chromatography-tandem mass spectrometryChromatography-tandem mass spectrometryAsymmetric dimethylarginineSymmetric dimethylarginineLC-MS/MS methodMass spectrometerFormic acidSpectrometry methodMass spectrometrySilica columnProtein precipitationSample preparationInternal standardAmmonium acetateMS methodGlomerular filtration rateAnalytesNitric oxide synthaseΜM