2022
Urinary Biomarkers of Tubular Health and Risk for Kidney Function Decline or Mortality in Diabetes
Chen T, Coca S, Thiessen-Philbrook H, Heerspink H, Obeid W, Ix J, Fried L, Bonventre J, El-Khoury J, Shlipak M, Parikh C. Urinary Biomarkers of Tubular Health and Risk for Kidney Function Decline or Mortality in Diabetes. American Journal Of Nephrology 2022, 53: 775-785. PMID: 36630924, PMCID: PMC10006337, DOI: 10.1159/000528918.Peer-Reviewed Original ResearchConceptsKidney function declineEnd-stage kidney diseaseFunction declineMCP-1Tubular healthUrinary biomarkersEGFR declineKIM-1YKL-40High riskChronic kidney disease progressionSubsequent eGFR declineKidney disease progressionGlomerular filtration rateYKL-40 levelsAvailable urine samplesOne-year changeHigher baseline levelsSubset of participantsBaseline UACRMedian UACRCause mortalityUrine albuminTubular injuryCreatinine ratio
2020
Uromodulin to Osteopontin Ratio in Deceased Donor Urine Is Associated With Kidney Graft Outcomes.
Mansour SG, Liu C, Jia Y, Reese PP, Hall IE, El-Achkar TM, LaFavers KA, Obeid W, Rosenberg AZ, Daneshpajouhnejad P, Doshi MD, Akalin E, Bromberg JS, Harhay MN, Mohan S, Muthukumar T, Schröppel B, Singh P, El-Khoury JM, Weng FL, Thiessen-Philbrook HR, Parikh CR. Uromodulin to Osteopontin Ratio in Deceased Donor Urine Is Associated With Kidney Graft Outcomes. Transplantation 2020, 105: 876-885. PMID: 32769629, PMCID: PMC8805736, DOI: 10.1097/tp.0000000000003299.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsBiomarkersCells, CulturedDelayed Graft FunctionFemaleGlomerular Filtration RateGraft SurvivalHistocompatibility Antigens Class IIHumansKidneyKidney TransplantationMacrophagesMaleMiceMiddle AgedOsteopontinProspective StudiesRisk AssessmentRisk FactorsTime FactorsTissue DonorsUnited StatesUromodulinConceptsGraft failureGraft functionGraft outcomePrimary outcomeMajor histocompatibilityDeath-censored graft failureDeceased donor kidney transplantationCause graft failureImproved graft survivalRecipient graft outcomesKidney graft outcomeGlomerular filtration rateDeceased donor kidneysTime of procurementGraft survivalKidney transplantationDirection of associationDeceased donorsFiltration rateKidney qualityLower riskExtensive injuryDonor urineSimilar associationMouse macrophages
2016
Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function
El-Khoury JM, Bunch DR, Hu B, Payto D, Reineks EZ, Wang S. Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function. Clinical Biochemistry 2016, 49: 1140-1143. PMID: 27452178, DOI: 10.1016/j.clinbiochem.2016.07.009.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateKidney functionCystatin CEGFR equationsWidespread clinical useIothalamate clearanceEGFR formulasFiltration rateSymmetric dimethylargininePlasma specimensAdult populationStrong markerAbstractTextClinical useEndogenous markerCreatinineLiquid chromatography-tandem mass spectrometry methodChromatography-tandem mass spectrometry methodFurther studiesDimethylarginineRoche Cobas 8000MarkersCatabolic productsCobas 8000
2011
A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized l-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges
El-Khoury JM, Bunch DR, Reineks E, Jackson R, Steinle R, Wang S. A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized l-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges. Analytical And Bioanalytical Chemistry 2011, 402: 771-779. PMID: 22124751, DOI: 10.1007/s00216-011-5462-9.Peer-Reviewed Original ResearchConceptsFast liquid chromatography–tandem mass spectrometry methodAnalytical cycle timeChromatography-tandem mass spectrometry methodLiquid chromatography-tandem mass spectrometry methodMinimum sample preparationMass spectrometry methodLiquid chromatography-tandem mass spectrometryChromatography-tandem mass spectrometryAsymmetric dimethylarginineSymmetric dimethylarginineLC-MS/MS methodMass spectrometerFormic acidSpectrometry methodMass spectrometrySilica columnProtein precipitationSample preparationInternal standardAmmonium acetateMS methodGlomerular filtration rateAnalytesNitric oxide synthaseΜM